15 results about "Enzymatic interesterification" patented technology

The invention discloses a method for preparing a functionalized polymer based on a macro-micro reactor chemical-enzyme method, which comprises the following steps: reacting a mixed solution of an acrylate monomer, an initiator, a ligand, a functional alcohol and a solvent in a micro reactor to obtain a reaction solution containing functionalized polyacrylate, and the micro reactor comprises the macro-micro reactor containing a copper internal member. In a macro-micro reactor, enzymatic ester exchange and Cu (0)-RDRP coupling reaction are carried out to obtain poly (methyl) acrylate with a series of chemical structures and copolymers of the poly (methyl) acrylate. The method is wide in applicable substrate range, and compared with a traditional synthesis method, the macro-micro reactor containing the internal component can remarkably shorten the polymerization time, secondary purification of the monomer is avoided, and the conversion rate is greatly increased. The characteristic scale of the macro-micro reactor reaches centimeter level, and high-flux synthesis can be realized. The invention provides a technical scheme for the preparation of the functionalized poly (methyl) acrylate polymer, and has an industrial conversion prospect.

The invention discloses a centimeter-level large-scale biosynthesis system based on a macro-micro reactor, which is characterized in that a mixed solution containing an acrylate monomer, an initiator, an RAFT (reversible addition-fragmentation chain transfer) reagent and functional alcohol reacts in the macro-micro reactor loaded with immobilized enzyme to obtain a functional polymer. According to the invention, enzymatic ester exchange and polymerization are carried out simultaneously in the macro-micro reactor to prepare the acrylate polymer. The macro-micro reactor containing the internal component can remarkably shorten the RAFT polymerization time and improve the polymerization conversion rate. The characteristic scale of the macro-micro reactor reaches centimeter level, and high-flux synthesis can be realized. The invention provides a technical scheme for the preparation of novel functionalized polyacrylate materials, and has industrial conversion prospects.

The invention discloses a lard liquid modification method based on enzymatic transesterification, and relates to the field of biochemistry, a solvent-free enzymatic transesterification reaction is adopted, lard and selected vegetable oil are mixed according to a preset mass ratio, and triacylglycerol molecular recombination is realized through a commercial lipase catalytic transesterification reaction. The modified lard oil is applied to food processing as a food base material or as a matrix of essence, perfume and lipophilic active molecules. The production process is simplified, the yield and stability of the liquid lard oil are improved, the problem that the lard oil is poor in flavor is solved, and the application market of the lard oil is widened.

The present invention relates to a process for producing a FAGE-based biofuel composition, the process being characterized by an enzymatic transesterification of fatty acid alkyl esters with glycerol formal. The invention is also directed to the biofuel composition obtained by said process as well as to the uses thereof.

The invention discloses an enzymatic preparation method for MLCT-rich grease and its product, comprising the following steps: (1) in a paraffin-water emulsion template system, preparing hydrophobic hollow SiO2 mesoporous microspheres (HMSB) as a hydrophobic carrier using an organic hydrophobic silicon source and a porogen in a one-step method; then immobilizing the free enzyme on the hydrophobic carrier by an adsorption method to obtain an immobilized lipase; (2) mixing long-chain triglycerides and a medium-carbon chain raw material, adding immobilized lipase, and performing an enzymatic transesterification reaction or an acidolysis reaction under a constant temperature water bath for a certain time; after the reaction is completed, solid-liquid separation is performed, and the obtained solid product is immobilized lipase, and the obtained solution is a crude grease product rich in MLCT, and then a small amount of fatty acids are removed by molecular distillation or physical adsorption to obtain a grease product rich in MLCT. The present invention has the advantages of wide raw material adaptability, high catalytic efficiency, high MCLT content, controllable functional fatty acid content, simple operation, green and environmental protection, and is suitable for large-scale amplification production.

The invention discloses a preparation method of zero-trans low-saturation high-beta'crystal form margarine, and belongs to the technical field of food processing. The method comprises the following steps: by taking camellia oil and palm stearin in a mass ratio of 7: 3 as base oil, adding 7.33% of immobilized lipase to carry out enzymatic transesterification, centrifuging to remove the lipase, adding 6% of glyceryl distearate to prepare base oil, mixing with 1% of sucrose fatty acid ester and 15% of water, emulsifying, quenching, kneading and curing to obtain a finished product. According to the invention, an enzymatic transesterification and oleogel dual structuring technology is adopted, and process parameters are accurately regulated and controlled, so that the product realizes zero trans-fatty acid residue, the saturation degree of transesterification grease is only 30.39%, the beta'crystal form proportion is high, and the caseinization property and the stability are excellent. The method is mild in process and easy to industrially popularize, and the prepared margarine can be widely applied to baked products such as cookies and bread and serves as an ideal substitute of animal butter.

The present disclosure is directed to a batch process for the enzymatic treatment of lipid-containing compositions, and more particularly, to a batch process for the manufacture of interesterified fats using enzymes. Advantageously, the process of the present disclosure can be performed using low amounts of enzymes, and shorter time frames than typical batch enzymatic interesterification processes. The claimed process can be used to replace batch chemical interesterification processes

The invention discloses zanthoxylum bungeanum flavor shortening based on enzymatic transesterification. The shortening comprises 25-45 parts of palm oil stearin; 25 to 45 parts of shea butter; 15 to 25 parts of soybean oil; 5-15 parts of zanthoxylum oil; the invention relates to a catalyst lipase for enzymatic transesterification. The invention also discloses a preparation method of the shortening, which comprises the following steps: respectively preheating the palm oil stearin, the shea butter, the soybean oil and the zanthoxylum oil according to the formula ratio at 65-75 DEG C, adding lipase for rotary evaporation after preheating, controlling the rotating speed at 180-220r / min, and reacting for 3-5 hours to complete the transesterification process; and then removing the lipase to terminate the reaction. According to the invention, palm oil stearin, shea butter and soybean oil are taken as substrates through an enzymatic ester exchange technology, zanthoxylum oil is innovatively introduced, and a novel shortening system with regional flavor is successfully developed. The acid value of the pepper-flavored shortening disclosed by the invention is between 0.2 mg / g and 0.5 mg / g, the peroxide value is between 0.05 g / 100g and 0.11 g / 100g, and both the acid value and the peroxide value meet food-grade requirements.

The invention relates to the technical field of food, in particular to a preparation method of low-fishy-smell high-stability fish oil. The method comprises the following steps: mixing fish oil with coconut oil to obtain a mixed solution; and heating the mixed solution to 50-70 DEG C in a protective gas atmosphere, adding lipase, and placing in a high-voltage electrostatic field for a period of time to obtain the low-fishy-smell high-stability fish oil. In the method, a mixed solution of fish oil, coconut oil and lipase is subjected to enzymatic transesterification, and high-voltage electrostatic field treatment is performed, so that the fishy smell of the fish oil is effectively reduced, and the volatile flavor and the structural stability of the fish oil are improved.

This invention discloses a centimeter-scale biosynthesis system based on a macro-microreactor. A mixed solution containing acrylate monomers, initiators, RAFT reagents, and functional alcohols is reacted in a macro-microreactor loaded with immobilized enzymes to obtain functionalized polymers. This invention enables the simultaneous enzymatic transesterification and polymerization of acrylate polymers within the macro-microreactor. The macro-microreactor with internal components significantly shortens the RAFT polymerization time and improves the polymerization conversion rate. The macro-microreactor's characteristic scale reaches the centimeter level, enabling high-throughput synthesis. This invention provides a technical solution for the preparation of novel functionalized polyacrylate materials and has promising prospects for industrial application.

To provide an efficient process for making a final plant oil composition in which at least 50% of the total palmitic acid in the triglycerides of the final plant oil composition is present in the sn2 position.SOLUTION: Disclosed is a process comprising providing a starting vegetable oil composition comprising palmitic acid in triglyceride, performing an enzymatic transesterification process, and using excess free fatty acids and / or non-glyceride esters thereof obtained during the process and recycling them back into the process, wherein at least 70 wt.% of the total amount of palmitic acid in the starting vegetable oil composition is present in the final vegetable oil composition. Further disclosed is a vegetable oil composition obtainable by the disclosed process, the use of the vegetable oil composition in the manufacture of an infant formula, and an infant formula comprising the vegetable oil composition.SELECTED DRAWING: Figure 4

The invention discloses a preparation method of an OPO-containing grease composition, which comprises the following steps: A, preparing raw materials for later use, including coconut oil, sunflower seed oil, palm stearin and lipase; b, taking all sunflower seed oil and part of palm stearin; c, adding the sunflower seeds and palm stearin taken in the step B into a reaction kettle, vacuumizing, heating, mixing, dehydrating and degassing to obtain a grease mixture; d, cooling the grease mixture, adding all lipase to carry out enzymatic transesterification, then cooling and crystallizing, and filtering out crystals for later use; e, adding all coconut oil and the rest palm stearin into the filtrate, heating, mixing, carrying out enzymatic transesterification again, cooling, crystallizing, and filtering out crystals for later use; and F, mixing the crystal filtered out in the step E and the crystal obtained in the step D, and purifying. According to the method, the defects in the prior art can be overcome, and the content of OPO in the prepared triglyceride is increased on the premise that the preparation cost is not obviously increased.

The present invention relates to a process for producing a FAGE-based biofuel composition, the process being characterized by an enzymatic transesterification of fatty acid alkyl esters with glycerol formal. The invention is also directed to the biofuel composition obtained by said process as well as to the uses thereof.

The invention belongs to the field of organic synthesis, and relates to a method for continuously preparing benzenesulfonic acid compounds through electrochemical-enzymatic coupling. Dissolving a compound 1 and a compound 2 in a first solvent to obtain a solution A; pumping the solution A into a first micro-reactor of a micro-channel reaction device, carrying out an electric cyclization reaction, and obtaining a first reaction solution after the reaction is finished; dissolving an oxidizing agent in a second solvent to obtain a solution B; respectively and simultaneously pumping the first reaction solution and the solution B into a second microreactor of the microchannel reaction device to carry out enzymatic transesterification, and obtaining a benzenesulfonic acid compound 4 after the reaction is finished; wherein the second microreactor is filled with lipase. The novel synthesis method provided by the invention adopts a strategy of combining electro-catalysis and enzyme catalysis, and compared with a traditional chemical synthesis method, the synthesis route provided by the invention does not use reagents with strong corrosivity, high toxicity and serious pollution, such as fuming sulfuric acid, hydrochloric acid, sulfur trioxide and the like, and is greener and safer.

The application discloses a method for preparing functionalized polymers based on macro-micro reactor chemical-enzyme method, and the method comprises the following steps: reacting a mixed solution of acrylate monomers, an initiator, a ligand, a functional alcohol and a solvent in a micro reactor to obtain a reaction liquid containing functionalized polyacrylate, wherein the micro reactor comprises a macro-micro reactor containing copper inner components. In the macro-micro reactor, enzymatic transesterification and Cu(0)-RDRP coupling reaction are coupled to obtain a series of poly(meth)acrylates and copolymers with chemical structures. Compared with traditional synthesis methods, the macro-micro reactor containing the inner components can significantly shorten the polymerization time, avoid secondary purification of monomers, and greatly improve the conversion rate. The characteristic scale of the macro-micro reactor reaches the centimeter level, and high-throughput synthesis can be realized. The application provides a technical scheme for preparing functionalized poly(meth)acrylate polymers, and has industrialization conversion prospects.