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Method for producing functional grease containing rich phytosterin ester from high-acid-value vegetable oil

A technology of phytosterol esters and high-acid value oils, which is applied in the direction of fermentation, can solve problems that have not yet been involved, and achieve the effects of reducing chemical deacidification steps, simple equipment, and mild reaction conditions

Active Publication Date: 2014-12-03
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports on the synthesis of phytosterol esters at home and abroad, but there is no research report on the preparation of functional oils rich in phytosterol esters using high acid value oils such as high acid value rice bran oil and wheat germ oil as raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for producing functional oil rich in phytosterol esters by utilizing high acid value vegetable oil, comprising the steps of:

[0026] (1) Pretreatment of raw materials: the reaction solvent (n-hexane) is dehydrated by anhydrous sodium sulfate (moisture<0.1wt%), and sitosterol (phytosterol) is vacuum-dried at 80°C for 12h (moisture<1wt%), high acid value The oil is rice bran oil (moisture <0.2wt%, acid value 20mgKOH / g);

[0027] (2) Input of reaction raw materials: 5L n-hexane, 207g phytosterol (sitosterol, 100mM), rice bran oil 1400g (acid value 20mgKOH / g, containing free fatty acid 100mM), molecular sieve (addition is 50g / L, i.e. 250g ) into the reaction kettle, adding 200ppm of natural vitamin E antioxidant, heating to 45°C, stirring and ultrasonic treatment (the time is 30 minutes, the power of ultrasonic treatment is 100w, and the ultrasonic frequency is 25Hz) to obtain a premix;

[0028] (3) Enzymatic transesterification reaction: add 25g (5g / L) Candida a...

Embodiment 2

[0031] A method for producing functional oil rich in phytosterol esters by utilizing high acid value vegetable oil, comprising the steps of:

[0032] (1) Pretreatment of raw materials: the reaction solvent (isooctane) adopts anhydrous sodium sulfate to remove water (moisture<0.1wt%), stigmasterol (phytosterol) is vacuum-dried at 100°C for 10h (moisture<1wt%), high acid Wheat germ oil (moisture <0.2wt%, acid value 40mgKOH / g) for oil and fat;

[0033] (2) Putting in the reaction raw materials: with 5L isooctane, 414g phytosterol (stigmasterol 200mM), 700g of wheat germ oil (acid value 40mgKOH / g, containing free fatty acid 100mM), molecular sieve (addition is 50g / L, namely 250g), add fat-soluble tea polyphenol antioxidant 150ppm, add to the reaction kettle, heat to 55 ° C, stir and sonicate (the time is 50 minutes, the power of sonication is 200w, and the ultrasonic frequency is 30Hz), to obtain the premix;

[0034](3) Enzymatic transesterification: 100 g (20 g / L) of Candida ant...

Embodiment 3

[0037] A method for producing functional oil rich in phytosterol esters by utilizing high acid value vegetable oil, comprising the steps of:

[0038] (1) Pretreatment of raw materials: the reaction solvent (n-heptane) uses anhydrous sodium sulfate to remove water (moisture<0.1wt%), brassicasterol is vacuum-dried at 120°C for 8h (moisture<wt%), and the high acid value oil is used Rice bran oil (moisture <0.2wt%, acid value 100mgKOH / g);

[0039] (2) Input of reaction raw materials: with 5L n-heptane, 828g phytosterol (brassicasterol 400mM), rice bran oil 280g (acid value 100mgKOH / g, containing fatty acid 80mM), molecular sieve (addition is 50g / L, namely 250g ), adding L ascorbyl palmitate antioxidant 200ppm, adding to the reaction kettle, heating to 60°C, stirring and ultrasonic treatment (the time is 60 minutes, the power of ultrasonic treatment is 300w, and the ultrasonic frequency is 40Hz), to obtain the premix;

[0040] (3) Enzymatic transesterification reaction: 200 g (40 ...

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Abstract

The invention relates to a method for producing functional grease containing rich phytosterin ester from high-acid-value vegetable oil. The method is characterized by comprising the following steps: (1) addition of reaction raw materials: adding phytosterin, high-acid-value grease and molecular sieve into a reaction kettle, adding 100-200ppm of antioxidant, heating to 45-60 DEG C, stirring and carrying out ultrasonic treatment to obtain a premixture, wherein the mole ratio of the phytosterin to free fatty acids in the high-acid-value grease is 1:1-5:1; (2) enzymatic ester exchange reaction: adding 5-40 g / L lipase into the premixture, and stirring to react at 45-60 DEG C under normal pressure for 6-12 hours; and (3) carrying out after-treatment on the product to obtain the functional grease containing rich phytosterin ester. The method can obviously lower the acid value of the high-acid-value vegetable oil and generate the new functional active component phytosterin ester, and the product is simple for separation and purification; and thus, the method can easily implement large-scale amplification.

Description

technical field [0001] The invention relates to a preparation method for producing functional oil rich in phytosterol esters by using high acid value vegetable oil, in particular to a method for preparing functional oil rich in phytosterol esters in one step by using high acid value vegetable oil as a raw material through an enzyme-catalyzed esterification method, which belongs to grease Deep processing and high-value utilization fields. Background technique [0002] The acid value of normal-produced vegetable oil is generally relatively low, but under special circumstances, such as high moisture in the oil, improper storage, and enzyme action during processing, etc., the acid value of the produced oil is relatively high, and the typical ones are high acid Wheat Germ Oil and High Acid Rice Bran Oil. High acid value oil mainly refers to oil with an acid value greater than 20 mgKOH / g. The representative of oil with high acid value - rice bran oil not only has a reasonable fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 黄凤洪郑明明黄清邓乾春向霞李文林时杰万楚筠
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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