Synthesized breast milk fat substitute product from mixed fatty acid under enzymatic condition in microwave and method for preparing breast milk fat substitute product

A technology of mixing fatty acids and breast milk fat, applied in the direction of fermentation, can solve problems such as unfavorable digestion and absorption of infants and young children, and achieve the effect of improving resource utilization and extending the industrial chain

Active Publication Date: 2016-01-06
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the characteristics of the composition and distribution of fatty acids in algal oil triglycerides, the sn-1 and 3 positions contain a ce

Method used

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  • Synthesized breast milk fat substitute product from mixed fatty acid under enzymatic condition in microwave and method for preparing breast milk fat substitute product
  • Synthesized breast milk fat substitute product from mixed fatty acid under enzymatic condition in microwave and method for preparing breast milk fat substitute product
  • Synthesized breast milk fat substitute product from mixed fatty acid under enzymatic condition in microwave and method for preparing breast milk fat substitute product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] This example illustrates the fatty acid composition analysis of substrates used for transesterification.

[0019] Olive oil, coconut oil, and algae oil were purified by urea inclusion method to obtain free fatty acids respectively, and the free fatty acids of the three oils were 70:15:15~98:1:1 according to the mass ratio (olive oil:coconut oil:algae oil ) after mixing through gas chromatography, obtain olive oil, coconut oil and algae oil and mixed free fatty acid (with mass ratio olive oil: coconut oil: algae oil=90:5:5 is example) the content of each fatty acid is shown in the table below 1. The mixed free fatty acids used in subsequent examples are all based on this example.

[0020] Content of each fatty acid in table 1 olive oil, coconut oil and algae oil and mixed oil (olive oil: coconut oil: algae oil=90:5:5)

[0021]

[0022] a: not detected

[0023] The following examples illustrate the process of using lipase as a catalyst to catalyze the esterification...

Embodiment 2

[0025]After mixing PPP (0.62g) and mixed free fatty acids (1.88g) at a molar ratio of 1:9, add sn-1, 3-position-specific lipase LipozymeRMIM10% (0.25g), and put it in a water bath shaker at 60°C for 3h .

[0026] After the reaction, take out 500 μL, add 60 mg of porcine pancreatic lipase to hydrolyze for 3 min, extract with ether, take the organic layer for thin-layer chromatography, and scrape the sn-2 monoglyceride band. Add 2 mL of n-hexane and 2 mL of 0.5 mol KOH-methanol solution and react in a water-bath shaker at 65°C for 60 minutes for methyl esterification, and then perform gas chromatography detection. The content of each fatty acid in the product oil is measured as shown in Table 2.

[0027]

Embodiment 3

[0029] After mixing PPP (0.74g) and mixed free fatty acid (1.76g) at a molar ratio of 1:7, add sn-1, 3-position specific lipase LipozymeRMIM6% (0.15g), and put it in a water bath shaker at 60°C for reaction 3h.

[0030] After the reaction, take out 500 μL, add 60 mg of porcine pancreatic lipase to hydrolyze for 3 min, extract with ether, take the organic layer for thin-layer chromatography, and scrape the sn-2 monoglyceride band. Add 2 mL of n-hexane and 2 mL of 0.5 mol KOH-methanol solution and react in a water-bath shaker at 65°C for 60 minutes for methyl esterification, and then perform gas chromatography detection. The content of each fatty acid in the product oil is measured as shown in Table 3.

[0031]

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PUM

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Abstract

The invention discloses a synthesized breast milk fat substitute product from mixed fatty acid under an enzymatic condition in microwave and a method for preparing the breast milk fat substitute product. The method includes respectively arranging molecular sieves and saturated salt solution in closed containers to control the activity of water; mixing olive oil with free fatty acid, coconut oil and algae oil with one another, mixing the olive oil with the free fatty acid, the coconut oil, the algae oil and triacylglycerol of palmitic acid with one another in the same reaction flasks and respectively arranging the reaction flasks and filter paper with lipase in the various closed containers and balancing the reaction flasks and the filter paper at the room temperatures; arranging substrates and the lipase in screwed glass bottles, closing the screwed glass bottles, carrying out enzymatic transesterfication reaction in constant-temperature water area shaking tables and microwave reactors, separating reaction grease by means of reduced-pressure distillation and refining the grease to obtain the breast milk fat substitute product. The olive oil with the free fatty acid is purified by the aid of a urea adduction process. The olive oil with the free fatty acid, the coconut oil, the algae oil and the triacylglycerol of the palmitic acid are used as the fatty enzymatic transesterfication substrates. The breast milk fat substitute product and the method have the advantages that the final reaction product contains abundant DNA (docosahexenoic acid), and the content of the palmitic acid on sn-2 sites of the breast milk fat substitute product is relatively high and can be assuredly equivalent to the content of palmitic acid on sn-2 sites of natural breast milk fat.

Description

technical field [0001] The invention relates to the field of biocatalysis, in particular to a breast milk fat substitute catalyzed by lipase to synthesize free fatty acid and palmitic triglyceride (tripalmitin, PPP) from mixed oil and a preparation method thereof. Background technique [0002] As an important nutrient during the growth and development of infants, breast milk fat plays an important role in the construction of infant cell membranes, vitamin absorption, energy supplementation and intellectual development. When the supply of breast milk is insufficient, or the baby needs to increase supplementary food after weaning, human milk fat substitute (HMFS) is used as an infant feeding food instead of breast milk, and its structure and function should be similar to natural breast milk fat, except for providing In addition to nutrients, infants and young children must be given more essential elements and organic substances that are conducive to their physical and intellec...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor 朱蔚杰王彦斋邢星星刘曦赵星宇朱丹梅艺苑王俊
Owner JIANGSU UNIV OF SCI & TECH
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