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Method of extracting CPT (camptothecin) from Mappia foetida by microorganism solid-state fermentation technology

A solid-state fermentation and microbial technology, applied in fermentation, organic chemistry and other directions, can solve problems such as unspecified extraction yield, and achieve the effects of improving plant resource utilization, reducing costs, and high biotransformation and extraction rates.

Inactive Publication Date: 2013-05-29
CHONGQING HUANSHAOTANG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

China Invention Patent Application No. 891012184, Inventor: Zhang Xiande, discloses a new method for extracting camptothecin from False Dragon tree. , and then repeatedly refined to obtain camptothecin. The mother liquor is recovered, treated and separated to obtain another active ingredient of antineoplastic drugs, methoxycamptothecin. The content of camptothecin in the whole plant of Pseudomonas chinensis proposed by this invention is 2-10 times higher than that of camptothecin, which is An important natural resource for extracting camptothecin found in our country, but the extraction rate is not stated, and the process is simple, and the data is not convincing. According to our research, the content of CPT in the Mabi wood plant is only the content of the root bark Higher, the content in branches and leaves is not higher than that of camptothecin, but 9-methoxycamptothecin is very rich

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  • Method of extracting CPT (camptothecin) from Mappia foetida by microorganism solid-state fermentation technology

Examples

Experimental program
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Embodiment 1

[0017] Follow the steps below:

[0018] (1) Fermentation: Take 100 parts by mass of mabi wood crushed into fine powder and put it in a container, add water to moisten it, then add 1-2 parts by mass of flavomycin, mix well, pile up the mixture and cover the container , control the temperature at 23-30°C, finish the fermentation after 72-120 hours, and add ethanol directly to the fermented mabi wood raw material to extract CPT or dry it for later use;

[0019] (2) Extraction: Take 100 parts by mass of fermented mabi wood raw material, add 750-850 parts by mass of ethanol with a concentration of 75%, extract by cold soaking for 3.5-4.5 hours, filter, and collect the filtrate; add 75% ethanol to the dregs 450-550 parts by mass, then extract for 2.5-3.5 hours, filter, and collect the filtrate; add 250-350 parts by mass of ethanol with a concentration of 75% to the dregs, extract for 1.5-2.5 hours, filter, and collect the filtrate; combine three ethanol extracts , concentrated unde...

Embodiment 2

[0021] Steps:

[0022] (1) Fermentation: Take 100kg of mabi wood crushed into fine powder and put it in a container, add water to moisten it, mix it with flavomycin and other compound enzymes, mix well, pile up and cover the surface, control the temperature at 23-30°C, and ferment and cultivate The culture can be terminated after 72-120 hours. The fermented mabi wood raw material can be directly added with ethanol to extract CPT or dried in the sun for later use.

[0023] (2) Extraction: Take 100kg of fermented mabi wood raw material, add 75% ethanol to 800L cold soak for 4 hours, filter and collect the filtrate. Add 500L of 75% ethanol to the dregs for extraction for 3 hours, filter, and collect the filtrate. Add 300L of 75% ethanol to the medicinal dregs for extraction for 2 hours, filter, and collect the filtrate. The three ethanol extracts were combined and concentrated under reduced pressure until the raffinate had no alcohol smell. Add water to the residual liquid to...

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Abstract

The invention discloses a method of extracting CPT (camptothecin) from Mappia foetida by a microorganism solid-state fermentation technology and relates to a method for extracting medicinal raw materials from traditional Chinese medicinal materials. The method includes: (1), fermentation: adding water to wet the Mappia foetida material which is pulverized into powders, then adding flavomycin, and performing fermental cultivating, and adding ethanol to extract CPT or drying to prepare; (2), extraction: adding ethanol to fermented Mappia foetida raw materials, performing cold-soaked extraction for 3.5-4.5 hours, and then filtering and collecting filtrate; repeating ethanol extraction for three times; combining ethanol filtrate prepared by three-time ethanol extractions together and vacuum-concentrating raffinate till no ethanol existing; adding water to the raffinate to 480-520 parts by mass, adjusting PH (potential of hydrogen) to 9-10, and absorbing by macroporous resin column; and eluting with ethanol, recycling ethanol, extracting with ethyl acetate, recycling ethyl acetate, crystallizing with methanol, recrystallizing with mecloqualone, and washing aciculas with distilled water, drying under reduced pressure, and finally obtaining the CPT with the purity of 98.7%. By the method, the technology of increasing camptothecin content in the raw materials is provided.

Description

technical field [0001] The invention relates to a method for extracting medicinal raw materials from Chinese medicinal materials, especially a method of using microbial solid-state fermentation technology to biotransform 9-methoxycamptothecin and other derivatives of camptothecin in the traditional Chinese medicine Mabimu, and convert derivatives similar to camptothecin into CPT Or HCPT, a method to increase the content of camptothecin. Background technique [0002] Camptothecin (CPT) is an alkaloid extracted from the fruit and leaves of the Chinese special plant Camptotheca acuminata Decne. [0003] CAS number: 7689-03-4, English name Camptothecin; [0004] Chemical name: 1H-Pyrano[34:6,7]indolizino[1,2-6]puinoline-3,14(4H,12H-)-dione,4-ethy1-4-hydroxy-(s), [0005] Molecular formula: C 20 h 16 N 2 o 4； Molecular weight: 348.34; [0006] Structural formula: [0007] Physical and chemical properties: light yellow needle crystal (methanol-acetonitrile), decompositio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C07D491/22
Inventor 阳水周远程陈忠琼
Owner CHONGQING HUANSHAOTANG BIO TECH
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