Technique for producing biologic diesel oil through combination of different lipases

A biodiesel and lipase technology, which is applied in the preparation of liquid hydrocarbon mixtures, petroleum industry, biological raw materials, etc., can solve the problems of low yield of biodiesel, and achieve the effect of reducing the cost of biological enzymes and increasing the yield.

Inactive Publication Date: 2005-10-26
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this single enzymatic process for the synthesis of b

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0018] Methanol and rapeseed oil with a molar ratio of 4:1 and 50% tert-butanol based on the oil volume were put into a stoppered conical flask, mixed evenly, and placed in a reciprocating shaker with automatic temperature control to heat to 40 After ℃, the immobilized lipase Lipozyme TL with 2% oil mass was added to start the reaction, and the reaction was carried out for 5 hours, and the yield of biodiesel was about 70%. The reaction mixture was taken out and the conversion was continued for 4 hours using another immobilized lipase Novozym 435 (0.5% based on the mass of the starting oil), and the yield of biodiesel was about 100%.

Embodiment 2

[0020] The ethanol and cottonseed oil with a molar ratio of 3.5:1, and 20% tert-butanol based on the oil volume were loaded into the enzyme reactor, the jacket temperature was controlled at 35°C, and the immobilized lipase Lipozyme RM with 1% oil mass was added. Start the reaction. After 4 hours of reaction, the yield of biodiesel is about 65%; the reaction mixture flows into another enzymatic reactor equipped with immobilized lipase Novozym 435 (0.5% based on the mass of starting oil) and continues to convert for 5 hours, and the biodiesel is converted for 5 hours. The yield is about 100%.

Embodiment 3

[0022] Methanol and oleic acid with a molar ratio of 3.5:1 were put into a stoppered conical flask and mixed evenly, and then placed in a reciprocating shaker with automatic temperature control and heated to 40°C, then immobilized fat with 1% oil mass was added. The enzyme Lipozyme TL started to react for 2 hours, and the yield of biodiesel was about 65%. Then the reaction mixture was taken out and treated with water-absorbing agents such as molecular sieve or silica gel (adding 3  molecular sieve or silica gel based on 2% oleic acid), The conversion was then continued for 5 hours with immobilized lipase Novozym 435 (0.2% based on the mass of the starting oil), and the yield of biodiesel was about 100%.

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Abstract

The present invention provides a process for producing biological diesel oil by utilizing combination of two kinds of several kinds of lipolases with different catalytic properties. In particular, it can combine the immobilized lipolase with 1,3-position specificity and the immobilized lipolase having not position specificity together and uses them simultaneously, and utilizes the catalytic property of different lipolases to make oil raw material effectively convert into biological diesel oil.

Description

technical field [0001] The invention belongs to the technical field of bio-oil synthesis, and in particular relates to a process for combining different lipases to produce biodiesel. The combination of lipases with different catalytic properties is used to catalyze the conversion of renewable oil raw materials into a new process for synthesizing biodiesel. Background technique [0002] The long-chain fatty acid esters produced by transesterification of bio-oil raw materials are a new type of non-polluting renewable energy, known as biodiesel. Its combustion performance is comparable to that of traditional petroleum-based diesel. At present, the research and application of biodiesel has received extensive attention. [0003] At present, biodiesel is mainly produced by chemical method, that is, transesterification is carried out with animal and vegetable oils and some low-carbon alcohols (methanol or ethanol) under the action of alkali or acid catalyst to generate correspondin...

Claims

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Application Information

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IPC IPC(8): C10G3/00
CPCY02E50/13Y02E50/10Y02P30/20
Inventor 刘德华杜伟李俐林王利李泽波
Owner TSINGHUA UNIV
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