Preparation method of magnetic hydroxyapatite immobilized lipase and application in edible fat and oil processing

A technology of immobilized lipase and hydroxyapatite, which is applied in the direction of immobilized on or in the inorganic carrier, edible oil/fat, application, etc., can solve the problems of use restrictions, increase the risk of cardiovascular and cerebrovascular diseases, etc., and achieve increased The effect of loading capacity, increasing contact mass transfer rate and uniformity, and good catalytic activity

Active Publication Date: 2016-01-20
唐河金海生物科技有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the trans fatty acids in hydrogenated fats can significantly increase

Method used

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  • Preparation method of magnetic hydroxyapatite immobilized lipase and application in edible fat and oil processing
  • Preparation method of magnetic hydroxyapatite immobilized lipase and application in edible fat and oil processing
  • Preparation method of magnetic hydroxyapatite immobilized lipase and application in edible fat and oil processing

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preparation example Construction

[0036] The preparation method of magnetic hydroxyapatite immobilized lipase of the present invention is as follows figure 1 shown, including the following steps:

[0037] The first step, the preparation of magnetic hydroxyapatite

[0038] A certain amount of FeCl 3 ·6H 2 O and Fe 2 SO 4 ·7H 2 O was put into a beaker, deoxygenated water was added, and mechanically stirred to make FeCl 3 ·6H 2 O and Fe 2 SO 4 ·7H 2 O is completely dissolved; then slowly add 25% NH 3 ·H 2 O solution in the mixed solution, stirred at 25°C for 30min; then slowly added Ca(NO 3 ) 2 4H 2 0 solution and (NH 4 ) 2 HPO 4 The solution is in the above reaction system, continue to stir for 30 minutes and then heat up to 90°C. After it has fully reacted, cool the product to room temperature and age overnight. The product is magnetically separated and washed with deionized water until it is neutral, and dried at 200°C. Calcined under the conditions for 3h to obtain magnetic hydroxyapatite ca...

Embodiment 1

[0051] (1) HAP-γ-Fe 2 o 3 Preparation of vector

[0052] Weigh 3.0gFeCl 3 ·6H 2 O and 1.54gFe 2 SO 4 ·7H 2 O in a 500mL beaker, add 90mL deoxygenated water, stir mechanically to make FeCl 3 ·6H 2 O and Fe 2 SO 4 ·7H 2 O is completely dissolved, then slowly add 30mL of 25% NH 3 ·H 2 O in the mixed solution, kept stirring at 25°C for 30 minutes, and 7.1g Ca(NO 3 ) 2 4H 2 O and 2.3g (NH 4 ) 2 HPO 4 Dissolve in 67mL of distilled water respectively, then slowly add these two solutions into the reaction system, continue stirring for 30min, then raise the temperature to 90°C, and react for 2h. After the reaction, the product was cooled to room temperature, aged overnight, the product was magnetically separated and washed with deionized water to neutrality, dried and calcined at 200°C for 3 hours to obtain HAP-γ-Fe 2 o 3 carrier.

[0053] (2) Amino functional modification of the carrier

[0054] Weigh 1.0gHAP-γ-Fe 2 o 3 The carrier was placed in a dry 100mL rou...

Embodiment 2

[0058] HAP-γ-Fe 2 o 3 The preparation and the amino functional modification method of the carrier are the same as in Example 1.

[0059] Immobilization of lipase: Lipase was also immobilized using glutaraldehyde as a cross-linking agent. Take 0.5g amino-modified HAP-γ-Fe 2 o 3 In a 100mL dry round bottom flask, add 10mL of 10% glutaraldehyde solution, shake in a constant temperature oscillator for 0.5h at 35°C, then add 10mL of lipase solution (0.2mol / L phosphoric acid at pH 7.0 AY lipase was added to the buffer solution at a ratio of 0.075g / mL and then mixed), and the shaking reaction was continued for 8h. After the reaction, the immobilized lipase was magnetically separated, washed with 0.2 mol / L phosphate buffer solution (pH=7.0), and freeze-dried to obtain the finished product of magnetically immobilized lipase—Catalyst 2#.

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Abstract

The invention discloses a preparation method of magnetic hydroxyapatite immobilized lipase. The method includes the steps of preparing a magnetic hydroxyapatite carrier so that lipase can be fixed to the carrier in the form of a chemical bond, conducting amino functional decoration on prepared magnetic hydroxyapatite, and immobilizing lipase with glutaraldehyde as cross-linking agent. The method has the advantages that magnetic nanometer particles are wrapped by hydroxyapatite, hydroxyapatite is provided with plentiful ducts, the inner walls of the ducts are provided with plentiful hydroxyl, further decoration of the carrier and immobilization of active substances are facilitated, and therefore the specific surface area of magnetic particles is greatly enlarged, the loading amount of lipase is increased, and the catalytic effect of immobilized lipase is better embodied; meanwhile, due to the good biocompatibility of hydroxyapatite, the catalytic activity of immobilized lipase is brought into better play.

Description

technical field [0001] The invention relates to a lipase catalyst, in particular to a preparation method of magnetic hydroxyapatite immobilized lipase, and also relates to the application of the prepared immobilized lipase in edible oil processing. Background technique [0002] As a catalyst, lipase is widely used in industries such as medicine, food, spices, oils, cosmetics and pesticides because of its high efficiency, specificity, mild reaction conditions, and environmental friendliness. However, free lipase has poor operational stability, is easy to inactivate, is not easy to reuse, is difficult to realize continuous and automatic production process, and is mixed into the product after reaction, making it difficult to separate. Therefore, although the catalysis technology of free lipase is relatively mature, the real industrial application is still greatly restricted. [0003] In order to overcome the defects of free lipase, there have been many reports on biological en...

Claims

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Application Information

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IPC IPC(8): C12N11/14A23D9/04
Inventor 谢文磊臧雪珍张弛王宏雁
Owner 唐河金海生物科技有限公司
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