Biological enzyme preparation method of natural brassinolide

A technology of brassinolide and biological enzyme, which is applied in the field of biotechnology separation and treatment, can solve the problems that the by-product waste cannot be comprehensively utilized, endanger the health of workers, and the pressure on environmental protection is high, and achieves outstanding physiological activity, low cost, and emphasis on control. effect of ability

Active Publication Date: 2010-03-31
CHENGDU NEWSUN CROPSCI
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  • Abstract
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Problems solved by technology

However, this method uses a large amount of carbon tetrachloride and chloroform as the extraction solvent, consumes a large amount of acid and alkali, has a huge pressure on environmental protection, seriously endangers the health of workers, pollutes the environment, and has a large production consumption, relatively high production costs, and by-products cannot be used as waste. Comprehensive utilization, unable to meet the needs of the market

Method used

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  • Biological enzyme preparation method of natural brassinolide
  • Biological enzyme preparation method of natural brassinolide

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[0033] 1. Fermentation and purification of highly selective esterase;

[0034] Fermentation enzyme production medium (fat 0.75%, NaNO 4 0.2%, Yeast Extract 0.5%, K 2 HPO 4 0.2%, pH7.5) sterilized at 120°C for 30 minutes, cooled to 28°C and inserted into seeds, stirred at 28-30°C for 45r / min. Aerated culture, the fermentation time is 76h.

[0035] The fermentation broth is added with flocculant and then centrifuged to remove bacteria to obtain crude enzyme liquid, which is filtered through a plate and frame filter press and then enters a hollow fiber concentration column for ultrafiltration concentration to obtain a concentrated enzyme liquid of 2000-2500U / L.

[0036] 2. High dispersion and emulsification treatment of beeswax:

[0037] For every 100L of water, add 30kg of beeswax and 0.8kg of emulsifier (for food), and emulsify at 80°C (60r / min) for 2h.

[0038]3. Directed hydrolysis of ester bonds in beeswax by esterase:

[0039] Add 0.4 times the amount of concentrated ...

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Abstract

The invention relates to a preparation method of a biological enzyme, particularly relates to a biological enzyme preparation method of natural brassinolide. The method includes: steps of inoculationfermentation, pressure filtration, ultrafiltration, emulsification, enzymolysis, extraction, pressure filtration, concentration, resin absorption, concentration crystallization, vacuum filtration, drying and packaging. The invention has advantages of simple process method, low energy consumption in production process, high product purity and environmental conservation.

Description

technical field [0001] The invention relates to a biotechnology separation and treatment method, in particular to a biological enzymatic hydrolysis preparation method of natural brassinolides, which are used to process brassinolides, which are steroidal plant growth regulators obtained from beeswax. Background technique [0002] Brassinolide (BR for short), also known as brassinolide, was first isolated and discovered in 1970 by Dr. Mirchell and others in the United States from rapeseed pollen. After in-depth research, it has been proved that brassinolide has a variety of unique physiological activities for regulating plant growth. It has stronger biological activity, lower usage, higher safety, and better effect on increasing production and income in agriculture. At the 8th International Plant Growth Substance Conference in 1982, it was unanimously recognized as a new generation of agricultural production materials, that is, the sixth largest plant growth regulator in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12R1/39
Inventor 何其明
Owner CHENGDU NEWSUN CROPSCI
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