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Method for producing fermentable sugars by hydrolysis of cellulosic component sulphonation separation couple enzyme

A cellulase and cellulose technology, applied in fermentation, biofuel and other directions, can solve the problem of low final yield and achieve the effect of improving the utilization rate of raw materials

Inactive Publication Date: 2009-03-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if there is no pretreatment process for effectively separating the components of the raw material, the enzymatic hydrolysis process will be very long, and the final yield of fermentable sugars such as hydrolyzed glucose will be very low (by weight, <30%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The cellulose raw material is wheat straw, and its cellulose content is 45%, hemicellulose content is 27%, lignin content is 17%, and the rest is ash and wax. Cut it into about 3cm long before use, wash it and prepare it by wet method. The cellulose raw material is added to the aqueous solution of sulfite and alkali compounds to carry out sulfonation reaction. Relative to the absolute dry weight of the cellulose raw material, the amount of sulfite is 8%, the amount of NaOH is 20%, and the amount of water added is 4 times. The sulfonation reaction temperature was 145°C, the heating time was 1h, and the holding time was 2.5h; the lignin removal rate of the sulfonation reaction was 91.2% (relative to the lignin content in the raw material); the hemicellulose hydrolysis rate was 34.3% (relative to the raw material The hemicellulose content); the cellulose yield was 92.4% (relative to the cellulose content in the raw material). After dehydration and washing, the insoluble c...

Embodiment 2

[0030] The cellulose raw material is masson pine wood chips, and in terms of weight ratio, the cellulose content is 42%, the hemicellulose content is 25%, the lignin content is 25%, and the rest is ash and wax. The size of wood chips is about 8cm 2 about. The cellulose raw material is added to the aqueous solution of sulfite and alkali compounds to carry out sulfonation reaction. In the sulfonation reaction system, relative to the absolute dry weight of the cellulose raw material, the amount of sulfite is 15%, the amount of NaOH is 20%, and the amount of anthraquinone is 20%. 1.0%, the amount of water added is 8 times. The sulfonation reaction temperature was 180°C, the heating time was 1.5h, and the holding time was 3.5h; the lignin removal rate was 81.7% (relative to the lignin content in the raw material); the hemicellulose hydrolysis rate was 43.8% (relative to the cellulose content); the cellulose yield is 95.6% (relative to the cellulose content in the raw material), t...

Embodiment 3

[0034] The cellulose raw material is straw, and the weight ratio is 40% of cellulose, 33% of hemicellulose, 15% of lignin, and the rest is ash and wax. Wash before use and cut into 3cm lengths. The cellulose raw material is added to the aqueous solution of sulfite and alkali compounds to carry out sulfonation reaction, relative to the absolute dry weight of the cellulose raw material, the amount of sulfite is 4%; NH 4 The amount of OH is 8%; the amount of anthraquinone is 0.3%, and the amount of water added is 4 times. The sulfonation reaction temperature was 120°C, the heating time was 1.0h, and the holding time was 3.0h; the removal rate of lignin was 91.2% (relative to the lignin content in the raw material); the hydrolysis rate of hemicellulose was 47.8% (relative to the hemicellulose in the raw material). cellulose content); cellulose yield 96.7% (relative to the cellulose content in the raw material).

[0035] After dehydration and washing, the insoluble cellulose prod...

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Abstract

The invention discloses a method for producing fermentable sugar through sulfonation, separation, coupling and enzymatic hydrolysis of cellulose compositions. The method comprises the following steps: firstly, cellulose raw materials are sliced or crushed and prepared by the pre-wet method; secondly, the cellulose raw materials are added into a water solution of sulfite and an alkaline compound, wherein the dosage of the sulfite is between 4 and 15 percent; the dosage of the alkaline compound is between 8 and 20 percent; the temperature of sulfonation reaction is between 120 and 180 DEG C; the reaction time, namely the heating-up time, is between 1 and 2 hours; and the soaking time is between 2 and 3.5 hours; and thirdly, cellulose products obtained through the sulfonation reaction which are not dissolved into a sulfonation reaction solution is dehydrated, cleaned and hydrolyzed in a cellulase reaction system which contains a buffer solution, and the fermentable sugar is obtained. The method can improve the yield of production of the fermentable sugar such as glucose, xylose and so on through transformation of the cellulose raw materials, improve the utilization rate of the raw materials, and achieve the aim of economy and high efficiency, and has important significance on promoting the commercial process of biomass energy - ethanol.

Description

technical field [0001] The present invention relates to a method for producing fermentable sugar by hydrolysis of cellulose trans-component sulfonation, separation and coupling enzyme. Background technique [0002] Energy shortage and environmental pollution are major challenges facing mankind. On the one hand, the fossil fuels that people depend on are facing depletion; on the other hand, the use of fossil fuels has also caused serious environmental pollution. For example, a large number of hydrocarbons, aromatics, phenolic compounds, as well as sulfur dioxide, nitrogen oxides and greenhouse gas emissions have threatened the living environment of human beings. Finding non-petroleum resources and non-petroleum energy to produce chemical products and fuels, developing renewable energy and its related industries, and creating a sustainable circular economy model have become a hot direction in the fields of energy, environment and chemical industry. [0003] As a clean and re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P7/06
CPCY02E50/17Y02E50/10
Inventor 林鹿庄军平
Owner SOUTH CHINA UNIV OF TECH
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