Method for producing soybean peptide

Abstract

The invention relates to a method for producing a soybean peptide. In the method, fermented soybean meal is taken as a raw material and then animal-derived protease is utilized to produce the soybean peptide. The method comprises the following steps of: 1) enzymolyzing liquid, namely mixing the fermented soybean meal and water in a weight ratio of 1:(4-8), adding trypsinase, and preserving heat and enzymolyzing; 2) killing enzyme at high temperature; 3) filtering, namely filtering and separating enzymatic hydrolysate to obtain crude soybean protein; 4) decolorizing and deodorizing; 5) refining; and 6) spray-drying, and sterilizing to obtain a finished product, wherein the decolorization and deodorization are realized through filtration by a pretreated macroporous resin. The method has theadvantages that: 1) the fermented soybean meal is taken as the raw material, so that a protease inhibitor in the raw material is eliminated; 2) the prehydrolysis of soybean protein in the fermented soybean meal can be completed; and 3) the amount of the additional enzyme is reduced greatly, so that the cost is effectively reduced and the higher hydrolysis rate is obtained.

Description

Technical field [0001] The invention relates to a soybean peptide production method, which uses fermented soybean meal as a raw material and reuses animal-derived protease to produce soybean polypeptide, and belongs to the technical field of microorganisms. Background technique [0002] Soy peptides are deep-processed products of soybeans. Soy protein is the most excellent plant protein, but because soybean contains a variety of anti-nutritional factors, it reduces the body's absorption and utilization rate. Because soybean protein is rich in resources, low in price, and rich in nutrients, the deep-processed soybean peptides have richer processing characteristics, nutritional characteristics and physiological functions than soybean protein. Soybean, soybean meal or soybean protein is used as the main raw material, using enzymatic hydrolysis Or peptides produced by microbial fermentation and with a molecular weight distribution below 10,000 Da are called soybean peptides. The am...

AI Technical Summary

Problems solved by technology

In the process of enzymatic hydrolysis, different enzymes are added at different stages. These enzymes include protease derived from wool enzyme, acid protease derived from Aspergillus niger, neutral protease derived from Aspergillus oryzae, and protease derived from bacteria (Alcalase, Alcakase, Protamex, etc.) , plant-derived proteases (papain, bromelain, etc.), but liquid enzymatic hydrolysis methods all have the problem of high production costs, which limits the application of soybean peptide products

Reasons for high cost: First, the liquid enzymatic hydrolysis method has a low hydrolysis absorption rate for low-priced crude materials such as soybean flour. Therefore, high-purity and high-priced raw materials are used, and soybean protein isolates are generally used as raw materials for production. The cost of the same amount of protein raw materials is higher than soybeans The powder has increased by more than 5 times; the second is that the amount of enzyme used in the liquid enzymatic hydrolysis method is large, and the cost of purchased enzyme is high