Silkworm chrysalis fibroin protein functional foods
A food and protein technology, which is applied in food preparation, animal protein processing, food science, etc., can solve the problems that affect the nutritional value and the effective play of physiological functions, and achieve the effect of low cost and high hydrolysis rate
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[0007] Pretreatment is carried out before enzymatic hydrolysis. The silk fibroin is coated with dead colloid. In order to fully enzymatically hydrolyze the silk fibroin, the wrapped sericin must be removed first, and the silk fibroin is dissolved to increase the contact with the enzyme. Before enzymatic hydrolysis, use 0.5% NaCO at 100°C 3 The solution was degummed from silkworm cocoons for 30 minutes at a solid-to-liquid ratio of 1:1.5, and filtered to obtain silk fibroin solids; the silk fibroin was dissolved in 55°C 40% CaCl 2 Aqueous solution (according to 1 part of silk fibroin: 10 parts of CaCl 2 (aqueous solution), after the silk fibroin is completely hydrolyzed (about 1h), use an ultrafiltration membrane to ultrafilter the silk fibroin solution to deionize small molecular substances such as pigments and odors. Hydrolyze at pH 7.5, 55°C for 120 minutes (enzyme addition 0.03g / 100mL), then use Flavourzyme500MG enzyme to hydrolyze 240mn at pH 6.5, 50°C (enzyme addition 0....
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