The invention provides a preparation method of glucose, and belongs to the technical field of glucose. The method comprises the steps of
starch milk preparation,
liquefaction, saccharification,
filtration and post-treatment. The filtering step comprises the following steps: filtering the saccharified liquid through a 0.5 mm screen, collecting primary filtrate of the saccharified liquid, then adding an
impurity removal agent, filtering, collecting secondary filtrate of the saccharified liquid, and carrying out
nanofiltration treatment to obtain saccharified filtrate; the preparation method of the
impurity removal agent comprises the following steps: placing
activated carbon in a
nitric acid solution, raising the temperature, stirring, filtering out, washing, putting into deionized water, adding a catalyst, stirring and catalyzing at
room temperature for 28-32 minutes, then adding lauryl amine and
glutamic acid, stirring and reacting at
room temperature, filtering, washing, putting into a
chitosan solution, stirring at 34-36 DEG C for 38-42 minutes, and filtering to obtain the
impurity removal agent. Filtering, washing and
drying to obtain an impurity removal agent; by adopting the preparation method disclosed by the invention, the yield and purity of the glucose are high, the stability of the functional saccharifying
enzyme is good, and the
energy consumption is low.