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Preparation method of compound protein peptide enzymatic hydrolysate capable of enhancing immunity, compound protein peptide beverage capable of enhancing immunity, and preparation method of compound protein peptide beverage

The technology of protease hydrolyzate and compound protein is applied in the preparation of immune-enhancing complex protein peptide enzymatic hydrolyzate, immune-enhancing complex protein peptide drink and preparation of immune-enhancing complex protein peptide drink, which can solve the problem of low utilization rate of digestion and absorption and high effect Poor and other problems

Pending Publication Date: 2020-09-18
RUNKE BIOENG FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The concept of supplementing collagen has been deeply rooted in the hearts of Chinese people for thousands of years. People usually supplement collagen through trotters, fish gelatin, donkey-hide gelatin, etc. However, due to the compact structure of collagen, the digestion and absorption utilization rate is low, and the effect is poor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] In this embodiment, the preparation method of the enhanced immune complex protein peptide hydrolyzate comprises the following steps:

[0075] (1) Based on dry weight, the following raw materials are prepared: 12% spirulina, 10% soybean protein isolate, 6% mung bean protein isolate, 6% wheat protein, 30% cod skin, 30% yak bone, and 6% whey protein ;

[0076] (2) Preparation of plant protein enzymatic hydrolyzate

[0077] (2-1) Spirulina pretreatment

[0078] Grind spirulina to obtain spirulina powder; then mix spirulina powder and purified water in a weight ratio of 1:8, stir evenly and then swell (stir at a speed of 50 rpm; swell at 50°C for 2 hours ); and then process the swollen spirulina powder with a high-pressure homogenizer (the pressure of the high-pressure homogenizer is 40MPa, and the treatment is 30 minutes) to obtain a pretreated feed liquid;

[0079] In this step (2-1), add spirulina powder and purified water into the stainless steel tank, the stainless s...

Embodiment 2

[0123] In this embodiment, the preparation method of the enhanced immune complex protein peptide hydrolyzate comprises the following steps:

[0124] (1) Based on dry weight, the following raw materials are prepared: 18% spirulina, 5% soybean protein isolate, 10% mung bean protein isolate, 10% wheat protein, 20% cod skin, 28% yak bone, and 9% whey protein ;

[0125] (2) Preparation of plant protein enzymatic hydrolyzate

[0126] (2-1) Spirulina pretreatment

[0127] Grind spirulina to obtain spirulina powder; then mix spirulina powder and purified water in a weight ratio of 1:6, stir evenly and then swell (stir at a speed of 40 rpm; swell at 65°C for 1.5 hours ); and then process the swollen spirulina powder with a high-pressure homogenizer (the pressure of the high-pressure homogenizer is 50MPa, and the treatment takes 25 minutes) to obtain a pretreated feed liquid;

[0128] In this step (2-1), add spirulina powder and purified water into the stainless steel tank, the stain...

Embodiment 3

[0171] Example 3 Enhancing Immunity Functional Test of Enzymatic Hydrolyzate of Complex Protein Peptide

[0172] The immune-enhancing complex protein peptide hydrolyzate obtained in Example 1 was used as the test substance to carry out the function test of enhancing immunity in mice. The protein peptide enzymatic hydrolyzate can be used as ordinary food according to the national announcement, and the recommended human intake is usually 3-5g / d (based on protein content) (the body weight is calculated as 60 kg, and the human body recommends the intake of immune-enhancing complex protein peptide enzymatic hydrolyzate The input amount is 0.35 ml / kg.bw / d (according to the protein containing 5 g / d). Specifically, follow the steps below:

[0173] One animal: BALB / c mice, male, weighing 18-22g; the mice were divided into six batches, 48 ​​in each batch; each batch was randomly divided into 4 groups according to body weight, 12 in each group; the first batch was used for organ / body we...

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Abstract

The invention discloses a preparation method of compound protein peptide enzymatic hydrolysate capable of enhancing immunity. The preparation method comprises the following steps of (1) preparing rawmaterials; (2) preparing plant protein enzymatic hydrolysate, wherein alkaline protease, flavourzyme and papain are used for enzymolysis in two steps; (3) preparing an animal protein enzymatic hydrolysate, wherein alkaline protease, trypsin and neutral protease are used for enzymolysis in two steps; (4) centrifuging; and (5) decolorizing and filtering to obtain the compound protein peptide enzymatic hydrolysate capable of enhancing immunity. The invention also provides a compound protein peptide beverage capable of enhancing immunity containing the compound protein peptide enzymatic hydrolysate, and a preparation method of the compound protein peptide beverage capable of enhancing immunity. Each protein can be fully subjected to enzymolysis, and the active peptide is reserved, so that theobtained compound protein peptide can be easily and quickly absorbed, and has the effects of quickly supplementing nutrition and improving immunity; the compound protein peptide beverage capable of enhancing immunity has a remarkable immunity-enhancing effect and is relatively delicious.

Description

technical field [0001] The present invention relates to the technical field of protein peptides and protein peptide drinks, in particular to a method for preparing an immune-enhancing complex protein peptide hydrolyzate, and an immune-enhancing complex protein peptide drink containing the immune-enhancing complex protein peptide enzymolyzate, and the A preparation method for enhancing immune complex protein peptide drink. Background technique [0002] Immunoactive peptides mainly refer to peptides that regulate the human immune system. To a certain extent, immunoactive peptides can enhance the immune ability of the body, enhance the phagocytosis of macrophages and the proliferation of lymphocytes and other physiological functions. Most of the immunoactive peptides have strong stability, small molecular weight, high biological activity and immunity. It has the advantages of weak originality, and can be made into various immune preparations and immune-enhancing health care pr...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23J3/16A23J3/18A23J3/14A23J3/20A23J3/06A23J3/04A23J3/08A23L33/18A23L33/15A23L33/105A23L2/02A23L2/52A23L2/66
CPCA23J3/346A23J3/347A23J3/342A23J3/341A23J3/16A23J3/18A23J3/14A23J3/20A23J3/06A23J3/04A23J3/08A23J3/343A23L33/18A23L33/105A23L33/15A23L2/02A23L2/52A23L2/66A23V2002/00A23V2250/55A23V2200/30A23V2250/21A23V2250/2124A23V2250/708A23V2200/14Y02P60/87
Inventor 许团辉陈璇李媛媛郑晓辉吴奕武陈蝶玲
Owner RUNKE BIOENG FUJIAN
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