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Method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici

A technology of Pediococcus lactis and chitin, which is applied in the direction of protein food ingredients, fish protein ingredients, and methods based on microorganisms, can solve the problems of nutrient loss and other problems, and achieve the goal of eliminating fishy smell, reducing costs, and improving extraction rate Effect

Inactive Publication Date: 2010-10-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for extracting protein and chitin from shrimp heads and shrimp shells according to the serious pollution and loss of nutrients in the existing methods for extracting protein and chitin from aquatic products. This method can obtain protein powder rich in calcium lactate without fishy smell from shrimp heads and shrimp shells, and can also obtain chitin at the same time. The method is fast and simple, reduces environmental pollution, and fully utilizes the rich nutrients

Method used

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Embodiment 1

[0025] In this embodiment, protein and chitin are extracted from shrimp heads and shrimp shells by fermentation of Pediococcus lactis. The specific steps are as follows:

[0026](1) Take 50g of fresh shrimp heads and shrimp shells, crush the shrimp heads and shrimp shells to about 0.5cm, add 25mL of water (50% of the total mass of the shrimp heads and shrimp shells), and adjust the pH to 6.4;

[0027] (2) add 7.5g glucose again (15% of shrimp head and shrimp shell gross mass);

[0028] (3) Pediococcus lactis is inoculated, and the inoculation amount is 5% of the total mass of the shrimp head and the shrimp shell;

[0029] (4) Insulated and fermented at 35°C for 5 days;

[0030] (5) After the fermentation is finished, centrifuge the fermented liquid and the fermentation residue; the fermented liquid is neutralized by sodium carbonate, concentrated, and spray-dried to obtain 7.2 g of powdered substance, which is the protein obtained in this embodiment;

[0031] (6) The ferment...

Embodiment 2

[0033] In this embodiment, protein and chitin are extracted from shrimp heads and shrimp shells by fermentation of Pediococcus lactis. The specific steps are as follows:

[0034] (1) Take 100g of fresh shrimp heads and shrimp shells, crush the shrimp heads and shrimp shells to about 0.5cm, and adjust the pH to 6.5;

[0035] (2) add 20g glucose again (20% of shrimp head and shrimp shell gross mass);

[0036] (3) Pediococcus lactis is inoculated, and the inoculation amount is 3% of the total mass of the shrimp head and the shrimp shell;

[0037] (4) Insulated and fermented at 30°C for 7 days;

[0038] (5) After the fermentation is finished, centrifuge the fermented liquid and the fermentation residue; the fermented liquid is neutralized by sodium carbonate, concentrated, and spray-dried to obtain 14.7 g of powdery substance, which is the protein obtained in this embodiment;

[0039] (6) The fermentation residue was soaked in 0.4 mol / L dilute hydrochloric acid for 6 hours, then...

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Abstract

The invention discloses a method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici, which comprises the following steps: (1) selecting fresh or frozen shrimp heads and shrimp shells, and unfreezing the frozen shrimp heads and the frozen shrimp shells; (2) adding carbon sources into the shrimp heads and the shrimp shells; (3) inoculating the pediococcus acidilactici; (4) carrying out heat-insulation fermentation; (5) separating fermentation liquid from residue after the fermentation is completed, and obtaining protein powder after the fermentation liquid is dried; and (6) carrying out diluted acid soaking and decolourization on fermentation residue to obtain chitin. Through the method of the invention, the proteins and the chitin in the shrimp heads and the shrimp shells can be simultaneously extracted out, and the obtained hydrolyzed proteins do not have stinking odor, and have rich calcium lactate and other mineral substances. The method of the invention has the advantages of simple operation method, production cost reduction and environmental pollution reduction, and rich nutritional ingredients contained in the shrimp heads and the shrimp shells can be sufficiently utilized.

Description

technical field [0001] The invention relates to the field of methods for extracting protein and chitin from aquatic products, in particular to a method for extracting protein and chitin from shrimp heads and shrimp shells. Background technique [0002] For shrimps, shrimp heads and shells are rich in nutrients. For example, the shrimp heads and shells of shrimps account for about 30% of the shrimp body weight. In addition to minerals such as chitin and calcium carbonate, they also contain 7~15% protein, 2~4% oil, rich in vitamin E, astaxanthin, lutein, lecithin and other ingredients, with high nutritional value. [0003] At present, the utilization of shrimp heads and shrimp shells is mainly in two aspects: one is to produce chitin, and the other is to use self-cathepsin or exogenous protease to hydrolyze the protein therein and liquefy and separate it. However, direct production of chitin from shrimp heads and shells requires high-concentration acid and alkali treatment of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04C12P19/04C12R1/01
Inventor 段杉李磊
Owner SOUTH CHINA AGRI UNIV
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