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Method for producing ethanol by using sugarcane bagasse cellulose and sugarcane molasses

A technology of sugarcane molasses and cellulose, which is applied in the fields of fermentation engineering, biomass energy, and biochemical industry. It can solve the problems of rising ethanol distillation and concentration costs, low substrate concentration, and poor infiltration, so as to improve comprehensive utilization and reduce Production cost, effect of reducing crystallinity

Inactive Publication Date: 2016-11-09
张聪聪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor wettability of lignocellulosic raw materials such as bagasse, the substrate concentration should not be too high during the hydrolysis and saccharification process, otherwise it will cause difficulties in stirring
However, if the substrate concentration is too low, the ethanol concentration in the fermented mash will hardly exceed 4%, which in turn will cause a substantial increase in the cost of ethanol distillation and concentration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for producing high-concentration ethanol by using bagasse cellulose and sugarcane molasses described in the present embodiment mainly includes the following steps:

[0029](1) Bagasse pretreatment: crush the bagasse with a pulverizer, pass through a 20-40 mesh screen, and immerse the crushed bagasse in a mixed solution of ferric chloride and sodium hydroxide, with a solid-to-liquid ratio of 1:10 , soak bagasse at room temperature for 20 hours; then heat up to 130°C and cook for 8 hours. After the reaction, the bagasse is washed with water until the lotion is neutral, and then decomposed into a fibrous slurry with a refiner, that is, bagasse pretreatment is completed. deal with;

[0030] (2) Bagasse cellulase hydrolysis and saccharification: put the pretreated fibrous slurry in a reaction tank, add sodium citrate or citric acid buffer according to the solid-to-liquid ratio of 1:25, and adjust the pH to 4.8-5.0, And add cellulase and xylanase mixed bacteria, s...

Embodiment 2

[0034] The method for producing high-concentration ethanol by using bagasse cellulose and sugarcane molasses described in the present embodiment mainly includes the following steps:

[0035] (1) Bagasse pretreatment: crush the bagasse with a pulverizer, pass through a 20-40 mesh screen, and immerse the crushed bagasse in a mixed solution of ferric chloride and sodium hydroxide, with a solid-to-liquid ratio of 1:15 , soak the bagasse at room temperature for 22 hours; then heat up to 135°C and cook for 10 hours. After the reaction, the bagasse is washed with water until the washing liquid is neutral, and then decomposed into a fibrous slurry with a refiner, that is, the bagasse pretreatment is completed. ;

[0036] (2) Bagasse cellulase hydrolysis and saccharification: put the pretreated fibrous slurry in a reaction tank, add sodium citrate or citric acid buffer according to the solid-to-liquid ratio of 1:26, and adjust the pH to 4.8-5.0, And add cellulase and xylanase mixed ba...

Embodiment 3

[0040] The method for producing high-concentration ethanol by using bagasse cellulose and sugarcane molasses described in the present embodiment mainly includes the following steps:

[0041] (1) Bagasse pretreatment: crush the bagasse with a pulverizer, pass through a 20-40 mesh screen, and immerse the crushed bagasse in a mixed solution of ferric chloride and sodium hydroxide, with a solid-to-liquid ratio of 1:18 , soak the bagasse at room temperature for 24 hours; then heat up to 138°C and cook for 11 hours. After the reaction, the bagasse is washed with water until the washing liquid is neutral, and then it is decomposed into a fibrous slurry with a refiner, and the bagasse pretreatment is completed. ;

[0042] (2) Bagasse cellulase hydrolysis and saccharification: put the pretreated fibrous slurry in the reaction tank, add sodium citrate or citric acid buffer according to the solid-liquid ratio of 1:28, adjust the pH=4.8-5.0, And add cellulase and xylanase mixed bacteria,...

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Abstract

The invention relates to a method for producing ethanol by using sugarcane bagasse cellulose and sugarcane molasses. The method comprises the following steps: sugarcane bagasse pretreatment, enzyme hydrolysis and saccharification of sugarcane bagasse cellulose, sugarcane molasses pretreatment and mixed fermenting of sugarcane bagasse cellulose hydrolysate and the sugarcane molasses. Yeast strains are fixed to carry out ethanol fermentation by using double-enzyme saccharification sugarcane bagasse cellulose and an adsorption fixing material, the density of the yeast strains can be increased, furthermore, the yeast strains can be repeatedly used, sugar consumed by yeast propagation is reduced, fermentation strength and yield of products are improved, the yield of the ethanol reaches 93.5%, comprehensive utilization of sugarcane bagasse and molasses resources is realized, comprehensive economic benefit of sugarcane sugar producing enterprises can be improved remarkably, and a win-win situation of economic benefit and economic benefit is realized.

Description

technical field [0001] The invention belongs to the fields of biochemical engineering, fermentation engineering and biomass energy, and in particular relates to a method for producing high-concentration ethanol by using bagasse cellulose and sugarcane molasses. Background technique [0002] Bagasse is the largest by-product of sugarcane sugar mills, with a yield of 24%-27% of the processed sugarcane. my country is the third largest sugarcane planting country in the world, and the output of bagasse in the south reaches 7000 kt / a. Bagasse is a rich renewable biomass resource, and its main components are natural polymer substances such as cellulose, hemicellulose and lignin. Among them, hemicellulose is mainly xylan, which is polymerized from xylose and arabinose, while cellulose is a polymer of glucose. At present, except for a small amount of bagasse used for papermaking, most of them are burned or discarded, which not only causes waste of resources, but also seriously poll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12P39/00C12N11/12C12N11/10C12R1/685C12R1/885
CPCC12N11/10C12N11/12C12P7/10C12P19/14C12P39/00C12P2201/00C12P2203/00Y02E50/10
Inventor 张聪聪
Owner 张聪聪
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