30results about How to "Improve ethanol yield" patented technology

Disclosed is a multiple adsorption tower alternate internal recycle method for producing anhydrous alcohol, which comprises the steps of heating, adsorbing, internal recycling, vacuum evacuating, flushing and resolving, the process has the advantages of extended adsorbent lifetime, reduced heat exchanger area, lowered cooling-water consumption, deep de-watering depth, and no pollution.

The invention relates to a method for producing ethanol by continuous enzymolysis and fermentation of lignocelluloses. The method comprises the following steps: (1) a pretreated lignocellulose raw material and an enzyme solution are added in proportion into an enzymolysis reactor with a screw device to carry out continuous enzymolysis; (2) the materials enter a fermentation tank I after enzymolysis, and microzyme is inoculated to carry out simultaneous saccharification and fermentation at 30-38 DEG C; (3) the materials in the fermentation tank I enter a fermentation tank II, and simultaneous saccharification and fermentation are continuously carried out at 39-44 DEG C; (4) the fermented materials undergo reduced pressure distillation; and (5) the distilled materials undergo solid-liquid separation, and an enzyme-containing liquid phase is reused in the step (1). By the use of the screw device, continuous supply and continuous enzymolysis of the raw materials are realized, and enzymolysis efficiency is enhanced. As simultaneous saccharification and fermentation are carried out at different temperatures, enzymolysis efficiency is further improved and yield of ethanol is then raised. The finally-obtained enzyme solution can be directly reused in the enzymolysis process, thus reducing production cost.

The invention relates to the technical field of spreading cultivation of microbial strains and fermentation of ethanol and discloses a method for spreading cultivation of saccharomycetes by virtue of liquefied mash, application of the method and a method for fermenting ethanol. The method for spreading cultivation of saccharomycetes by virtue of liquefied mash comprises the step of carrying out intermittent spreading cultivation and fed-batch spreading cultivation on activated saccharomycetes by utilizing liquefied mash filtrate under an aseptic condition. According to the method, the number of the saccharomycetes can be effectively controlled, the obtained saccharomycetes have relatively high activities, and the yield of ethanol and the consumption rate of xylose in a fermentation process can be obviously increased.

The invention relates to a method for producing fuel ethanol by sugar grass stalk juice and a device; the stalk pretreatment, juice modulation, immobilization yeast particles and fluidization quick fermentation new processes are adopted to prepare the fuel ethanol; the fermentation process is continuous, the equipment is advanced, the fermentation speed is rapid, the reaction is full, the output is high, and the ethanol yield is high, no three-waste exhaust exists, secondary utilization is carried out totally, a great amount of investment, labor cost and raw material cost are reduced, the economic benefit is improved obviously, and the production cost is greatly reduced.

The invention belongs to the technical field of preparing fuel ethanol by enzymatic hydrolysis and fermentation of lignocellulose, and discloses a method for improving the yield of ethanol produced by enzymatic hydrolysis and fermentation of lignocellulose by a lignin amphiphilic polymer. The specific steps of the method are: dispersing 100 mass parts of pretreated lignocellulose in a buffer solution of 330 to 5000 mass parts, adding 0.5 to 50 mass parts of lignin amphiphilic polymer, and then adding 5 to 30 FPU / g cellulase (based on the mass of dry lignocellulose), and then add Saccharomyces cerevisiae, and react at 30-45°C for 48-120h to obtain a fermentation broth. The invention can not only effectively improve the ethanol yield of lignocellulose enzymolysis fermentation, but also does not need additional equipment, and has simple process and convenient operation.

The invention discloses a saccharomyces cerevisiae strain expressing xylose isomerase and a construction method. The saccharomyces cerevisiae (SEB8) strain expressing the xylose isomerase is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC11328. The invention further provides the construction method of the saccharomyces cerevisiae (SEB8). The obtained strain (SEB8) can generate ethanol with the concentration of 14.73 g / L from xylose with the concentration of 33.71 g / L in 48 hours of fermentation time, and the ethanol yield reaches 0.44 gram per gram of the xylose. The strain has the advantages of being high in xylose fermentation speed and ethanol yield and the like, can produce the fuel ethanol from saccharification liquid prepared by taking lignocellulose as a raw material, greatly reduces the production cost of the fuel ethanol and has a wide application prospect.

The invention discloses an alcohol production method, which comprises the following steps of: (1) respectively carrying out liquefaction and saccharification on farinaceous size, and fermenting a saccharification liquid obtained by saccharification to obtain CO2-containing gas component A and a fermented liquor; (2) letting the CO2-containing gas component A contact with a washing liquid, washing, dissolving ethanol from the CO2-containing gas component A in the washing liquid to obtain a complete liquid; and carrying out solid-liquid separation on the fermented liquor to obtain a fermented clear liquid; (3) carrying out distillation on the complete liquid and the fermented clear liquid to obtain a gaseous distillate B, condensing the gaseous distillate B until ethanol is a liquid state, so as to obtain a liquid ethanol fraction C and a gas component D; and (4) letting the gas component D contact with water, and washing to obtain a washed liquid E. The invention is characterized in that the washing liquid comprises the washed liquid E and the concentration of ethanol in the washing liquid is not higher than 2.5% (volume). The alcohol production method provided by the invention is used to raise ethanol yield and reduce cost.

The present invention discloses a Saccharomyces cerevisiae strain and construction method that can rapidly ferment xylose to produce ethanol, discloses a Saccharomyces cerevisiae strain that can simultaneously ferment glucose, xylose and cellobiose to produce ethanol, and solves the problem of Saccharomyces cerevisiae The problem of inability to produce ethanol from xylose and cellobiose. The invention includes that the strain of Saccharomyces cerevisiae SEB3 is preserved in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC11323. The invention also provides the construction method of the Saccharomyces cerevisiae SEB3. The present invention has the advantages of excellent xylose fermentation ability, fast xylose consumption rate and high ethanol yield; at the same time, the bacterial strain SEB3 of the present invention has good acid resistance and temperature resistance, and the pH value is not lower than 3 and the temperature is not high. At 35°C, pH and temperature had no significant effect on the fermentation rate and efficiency of xylose and cellobiose.

The invention provides a method for promoting poplar ethanol production by pretreatment of hydrothermal combined with NaOH aqueous solution. In the present invention, the poplar is first crushed, reacted with water at 180-200 DEG C for 5-30 minutes, the separated filter residue is mixed with NaOH aqueous solution, reacted at 80-120 DEG C for 30-120 minutes, and the pretreatment residue is obtained by separation, Then add cellulase, yeast activation solution and nutrient solution for saccharification and fermentation. The method adopts the combination of two stages of pretreatment of hydrothermal and NaOH aqueous solution to reduce the influence of lignin on the enzymatic hydrolysis and fermentation process, and under the premise of reducing the alkali concentration, it can also effectively promote the production of ethanol from poplar, and the ethanol yield is as high as 50.91%.

The invention discloses a method for improving the xylose utilization ability of a recombinant Saccharomyces cerevisiae strain by using a transcription factor Tec1p mutant or using xylose-containing raw materials to produce ethanol. To improve the ability of strain xylose utilization; the transcription factor Tec1p mutant is named as Tec1p T273M , is formed by mutating the threonine at position 273 of the wild-type transcription factor Tec1p whose amino acid sequence is SEQ ID NO:1 to methionine, and its amino acid sequence is shown in SEQ ID NO:2. Experiments show that the growth of the mutant strain is significantly better than that of the starting strain BSGX001 in the co-fermentation of glucose and xylose by the method of the present invention, and the utilization of xylose and the production rate of ethanol are significantly higher than those of the control strain.

The present invention discloses a strain of Saccharomyces cerevisiae expressing xylose isomerase and its construction method, which solves the problem of existing xylA After the gene is heterologously expressed in Saccharomyces cerevisiae, the growth rate of the recombinant bacteria constructed by it is relatively slow in xylose, which cannot meet the needs of fermentation. The present invention includes said Saccharomyces cerevisiae ( Saccharomyces cerevisiae The SEB7) strain is preserved in the China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC11327. The invention also provides the construction method of the Saccharomyces cerevisiae SEB7. The bacterial strain SEB7 that the present invention obtains can utilize 16.95g / L xylose to produce the ethanol of 6.98 g / L in the fermentation time of 48 h, and ethanol yield reaches 0.412 g / g xylose; This bacterial strain has xylose fermentation fast, The advantages of high ethanol yield.

The invention low temperature-resistant Saccharomyces cerevisiae JM33 and application of the low temperature-resistant Saccharomyces cerevisiae JM33 in munake grape wine. According to the low temperature-resistant Saccharomyces cerevisiae JM33 disclosed by the invention, by carrying out strain separating, screening, breeding and acclimatizing on munake grapes and carrying out morphological characteristic and 26S rDNA (Ribosomal Deoxyribose Nucleic Acid) sequence determination and phylogenetic analysis on an obtained strain, a fermentation action of strain Saccharomyces cerevisiae JM33 CGMCC NO.10912 on the munake grapes is determined; the munake grape wine is prepared by fermenting the munake grapes by utilizing the Saccharomyces cerevisiae JM33 CGMCC NO.10912, the munake grape wine is straw-like yellow and is clear and transparent, the fruity flavor is strong, the taste is mellow and soft, and a special flavor of the munake grape wine is obtained.