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Method for improving ethanol production efficiency of lignocellulose enzymatic fermentation

A lignocellulosic enzyme and lignocellulose technology, applied in fermentation, biofuels, etc., can solve problems such as difficult degradation of surfactants, reduced use efficiency, and ecological system pollution, so as to reduce ineffective adsorption, improve efficiency, and promote The effect of mass transfer

Pending Publication Date: 2020-06-30
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, chemically synthesized surfactants are difficult to degrade when discharged into the environment, which poses environmental protection pressure and will cause certain pollution to the ecosystem
On the other hand, surfactants will also be adsorbed by lignocellulose during use, which also reduces their use efficiency to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The corn stalks were adjusted to a water content of 88%, soaked for one hour, put into the feeder of a steam explosion device, and extruded by a screw into a digester. The cooking temperature was 180°C and the cooking time was 10 minutes. The material in the digester is extruded by a combined screw device, and enters the retainer at a temperature of 200°C and a pressure of 1.8 MPa for 10 minutes, and then the pressure is released and exploded instantly to obtain the material pretreated by steam explosion.

[0027] The steam explosion pretreated material and water were prepared into a mixed solution with a solid-liquid mass ratio of 12.6%, hydroxypropyl-β-cyclodextrin with 0.2% cellulose dry matter was added, and Novozymes’ Ctec2 cellulase was added , the addition amount is that every gram of cellulose adds the cellulase liquid of 10 FPU unit enzyme activity. Under the conditions of 50°C, pH 5.0, and rotation speed 130rpm, the enzyme was hydrolyzed for 120h.

[0028] Ad...

Embodiment 2

[0032] Same as Example 1, the difference is that no hydroxypropyl-β-cyclodextrin was added in the ethanol fermentation. From the results of enzymatic hydrolysis, the enzymatic hydrolysis of the pretreated straw yielded 47.5g / L of glucose, with a yield of 79.4%; 24.4g / L of xylose, with a yield of 87.2%. From the fermentation results, the ethanol concentration is 24.7g / L, the conversion rate of glucose is 90.2%, and the conversion rate of xylose is 61.8%.

Embodiment 3

[0034] Same as Example 1, the difference is that no hydroxypropyl-β-cyclodextrin is added in the enzymatic hydrolysis process. From the results of enzymatic hydrolysis, the enzymatic hydrolysis of pretreated straw yielded 39.1 g / L of glucose, with a yield of 66.0%; 21.0 g / L of xylose, with a yield of 75.2%. From the fermentation results, the ethanol concentration is 22.2g / L, the conversion rate of glucose is 89.5%, and the conversion rate of xylose is 63.2%.

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PUM

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Abstract

The invention relates to a method for improving ethanol production efficiency of lignocellulose enzymatic fermentation. The method comprises the following steps: firstly, uniformly mixing pretreated lignocellulose with water to prepare a mixed solution; then adding cellulase and cyclodextrin to the mixed solution for enzymolysis treatment; and inoculating an enzymatic hydrolysate with ethanol fermentation strains for ethanol fermentation. The method can reduce the influence of pretreatment inhibitors on enzymolysis and ethanol fermentation, and improve enzymolysis efficiency and enzymolysis sugar conversion rate.

Description

[0001] field of invention [0002] The invention belongs to the technical field of biomass energy, and in particular relates to a method for improving the ethanol production efficiency of lignocellulose enzymatic fermentation. Background technique [0003] In recent years, with the continuous consumption of fossil energy and the increasingly prominent environmental problems, the development of biomass energy has attracted widespread attention from all walks of life around the world. Since the 1970s, bioenergy represented by fuel ethanol and biodiesel has been vigorously developed. The development of the first generation of biomass fuels is based on the large occupation and consumption of agricultural resources, which has triggered a series of concerns about "food security". "Questions of debate. The development of non-food biofuels (second-generation biofuels) has become an important topic of concern worldwide, and it is also an important direction for the development of biof...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCC12P7/10C12P2201/00Y02E50/10
Inventor 高慧鹏张全关浩王蒙彭绍忠
Owner CHINA PETROLEUM & CHEM CORP
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