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A method for liquefied mash expanding yeast and its application and method for fermenting ethanol

A technology for liquefying mash and yeast, applied in fermentation, fungi, biofuels and other directions, can solve the problems of low yeast activity and low utilization rate of ethanol fermentation sugars, and improve ethanol yield and xylose consumption rate. , the effect of high vitality

Active Publication Date: 2020-04-17
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, for the aforementioned two expansion methods, on the one hand, it is difficult to effectively control the amount of yeast obtained, which often leads to overcultivation (the number of yeast in the seed liquid of expansion is at least 300 million / ml), resulting in a considerable amount of yeast during fermentation. The sugars in the yeast are used for the metabolism of the yeast cell itself, resulting in lower utilization of sugars for ethanol fermentation; Consumption rate) is lower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Under aseptic conditions, inoculate the activated yeast liquid into a 10L seed tank with 6L corn liquefaction mash filtrate with an inoculation amount of 5% by volume for intermittent expansion. At 30°C, the ventilation rate is 0.1VVM, and the intermittent expansion is stopped when the number of yeast in the seed liquid is 120 million / ml. At this time, the volume of the seed liquid in the seed tank is 6L.

[0036] Carry out fed-batch expansion culture at the end of batch culture, including: in the seed tank after batch culture ends, flow into the fresh liquefied mash filtrate and flow out seed liquid into the 30L fermenter simultaneously with the volume flow rate of 0.6L / h, wherein, fed-batch The culture conditions for the expanded culture include: the temperature is 30° C., the pH value is 5.0, and the ventilation volume is 0.1 VVM. After testing, in the whole feeding process, the number of yeasts in the seed solution fed into the fermenter is 1.9±0.1 billion / ml, the y...

Embodiment 2

[0040] Under sterile conditions, inoculate the activated yeast liquid into a 10L primary seed tank equipped with 6L corn liquefaction mash filtrate with an inoculation amount of 3% by volume to carry out a primary intermittent expansion culture, and then inoculate the seed liquid obtained from the primary culture Inoculate the 10L secondary seed tank with 6L corn liquefaction mash filtrate with the inoculum amount of 3 volume % to carry out the secondary batch expansion culture, wherein, the culture conditions of the two-stage batch culture include: the temperature is 29 ℃, the ventilation rate is equal to Be 0.12VVM, stop cultivating when cultivating to the yeast number in the seed liquid all is 150 million / ml, after the secondary intermittent expansion finishes, the volume of the seed liquid in the secondary seed tank is 6L.

[0041] At the end of the second batch culture, the fed-batch expansion culture is carried out, including: in the second seed tank after the second batc...

Embodiment 3

[0045] Under aseptic conditions, inoculate the activated yeast liquid into a 10L seed tank equipped with 6L corn liquefaction mash filtrate with an inoculation amount of 6% by volume for intermittent expansion. The temperature is 28°C, the ventilation rate is 0.15VVM, and the culture is stopped when the number of yeasts in the seed solution is 100 million / ml. After the intermittent expansion, the volume of the seed solution in the seed tank is 6L.

[0046] Carrying out fed-batch expansion at the end of batch cultivation, including: inflowing fresh liquefied mash filtrate and flowing out seed liquid into a 30L fermenter simultaneously with a volume flow rate of 0.75L / h in the seed tank after batch cultivation, wherein, feeding The culture conditions for the expanded culture include: the temperature is 28° C., the pH value is 4.5, and the ventilation volume is 0.15 VVM. After testing, the number of yeasts in the seed liquid fed into the fermenter is 205±0.15 billion / ml during th...

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Abstract

The invention relates to the technical field of spreading cultivation of microbial strains and fermentation of ethanol and discloses a method for spreading cultivation of saccharomycetes by virtue of liquefied mash, application of the method and a method for fermenting ethanol. The method for spreading cultivation of saccharomycetes by virtue of liquefied mash comprises the step of carrying out intermittent spreading cultivation and fed-batch spreading cultivation on activated saccharomycetes by utilizing liquefied mash filtrate under an aseptic condition. According to the method, the number of the saccharomycetes can be effectively controlled, the obtained saccharomycetes have relatively high activities, and the yield of ethanol and the consumption rate of xylose in a fermentation process can be obviously increased.

Description

technical field [0001] The invention relates to the technical field of microbial strain expansion and ethanol fermentation, and in particular relates to a method for expanding and cultivating yeast with liquefied mash and its application and a method for fermenting ethanol. Background technique [0002] my country is a big agricultural country, and the output of crop straw is about 700 million tons per year, ranking first in the world. At present, the main utilization methods of crop straw are: returning fertilizer (directly returning to the field, organic fertilizer), rural energy (gasification, combustion), livestock feed (direct feeding, fertilizer after treatment), industrial raw materials (papermaking raw materials, cultivation Edible mushrooms) and so on. However, still about 20% of the total straw is discarded in fields and ditches, or burned. According to the current level, about 6 tons of straw can produce 1 ton of fuel ethanol. If our country utilizes straw and l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/10
CPCC12N1/16C12P7/10Y02E50/10
Inventor 袁敬伟李春玲杨宇平孙岩张宁李杰李凡沈乃东熊强
Owner COFCO NUTRITION & HEALTH RES INST
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