A method for liquefied mash expanding yeast and its application and method for fermenting ethanol

Abstract

The invention relates to the technical field of spreading cultivation of microbial strains and fermentation of ethanol and discloses a method for spreading cultivation of saccharomycetes by virtue of liquefied mash, application of the method and a method for fermenting ethanol. The method for spreading cultivation of saccharomycetes by virtue of liquefied mash comprises the step of carrying out intermittent spreading cultivation and fed-batch spreading cultivation on activated saccharomycetes by utilizing liquefied mash filtrate under an aseptic condition. According to the method, the number of the saccharomycetes can be effectively controlled, the obtained saccharomycetes have relatively high activities, and the yield of ethanol and the consumption rate of xylose in a fermentation process can be obviously increased.

Description

technical field

[0001] The invention relates to the technical field of microbial strain expansion and ethanol fermentation, and in particular relates to a method for expanding and cultivating yeast with liquefied mash and its application and a method for fermenting ethanol. Background technique

[0002] my country is a big agricultural country, and the output of crop straw is about 700 million tons per year, ranking first in the world. At present, the main utilization methods of crop straw are: returning fertilizer (directly returning to the field, organic fertilizer), rural energy (gasification, combustion), livestock feed (direct feeding, fertilizer after treatment), industrial raw materials (papermaking raw materials, cultivation Edible mushrooms) and so on. However, still about 20% of the total straw is discarded in fields and ditches, or burned. According to the current level, about 6 tons of straw can produce 1 ton of fuel ethanol. If our country utilizes straw and l...