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A Saccharomyces cerevisiae strain and its construction method for rapidly fermenting xylose to produce ethanol

A Saccharomyces cerevisiae strain and technology of Saccharomyces cerevisiae are applied in the field of Saccharomyces cerevisiae strains that rapidly ferment xylose to produce ethanol, can solve the problems of inability to ferment xylose and cellobiose to produce ethanol, etc., and achieve high ethanol yield and xylose consumption rate The effect of fast and excellent xylose fermentation capacity

Active Publication Date: 2019-01-04
SICHUAN UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that existing Saccharomyces cerevisiae cannot ferment xylose and cellobiose to produce ethanol; provide a Saccharomyces cerevisiae strain that can simultaneously ferment glucose, xylose and cellobiose to produce ethanol that solves the above problems

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  • A Saccharomyces cerevisiae strain and its construction method for rapidly fermenting xylose to produce ethanol
  • A Saccharomyces cerevisiae strain and its construction method for rapidly fermenting xylose to produce ethanol
  • A Saccharomyces cerevisiae strain and its construction method for rapidly fermenting xylose to produce ethanol

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Embodiment

[0050] A Saccharomyces cerevisiae strain that rapidly ferments xylose to produce ethanol, the Saccharomyces cerevisiae ( Saccharomyces cerevisiae The SEB3) strain is preserved in the China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC11323. The specific construction method of the Saccharomyces cerevisiae strain SEB3 is as follows:

[0051] 1) Construct the host strain

[0052] 1.1) Construct the starting strain: Saccharomyces cerevisiae Saccharomyces cerevisiae IR-2 and Saccharomyces cerevisiae SEB1 was the parent, and the starting strain RHZ-1 was obtained through cell fusion;

[0053] 1.2) After ultraviolet mutagenesis of the starting strain RHZ-1, a uracil-deficient strain 1 ( ura3 / ura3 );

[0054] 1.3) obtained by PCR amplification from the genomic DNA of strain 1 using primers TRP1-437F and TRP1-93R TRP1The upstream fragment of the gene; amplified from plasmid PET01 using primers TRP1PTEF1-F and ERG25 rev...

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Abstract

The present invention discloses a Saccharomyces cerevisiae strain and construction method that can rapidly ferment xylose to produce ethanol, discloses a Saccharomyces cerevisiae strain that can simultaneously ferment glucose, xylose and cellobiose to produce ethanol, and solves the problem of Saccharomyces cerevisiae The problem of inability to produce ethanol from xylose and cellobiose. The invention includes that the strain of Saccharomyces cerevisiae SEB3 is preserved in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC11323. The invention also provides the construction method of the Saccharomyces cerevisiae SEB3. The present invention has the advantages of excellent xylose fermentation ability, fast xylose consumption rate and high ethanol yield; at the same time, the bacterial strain SEB3 of the present invention has good acid resistance and temperature resistance, and the pH value is not lower than 3 and the temperature is not high. At 35°C, pH and temperature had no significant effect on the fermentation rate and efficiency of xylose and cellobiose.

Description

technical field [0001] The invention relates to a yeast strain, in particular to a Saccharomyces cerevisiae strain for rapidly fermenting xylose to produce ethanol and a construction method of the strain. Background technique [0002] Fuel ethanol, as a biofuel that can replace gasoline, is a kind of renewable clean energy vigorously developed by countries all over the world. The production of fuel ethanol from lignocellulosic biomass such as agricultural straw has the characteristics of "not competing with people for food and land", and is the main direction of fuel ethanol production and development in my country. Cellulose and hemicellulose in lignocellulosic biomass are hydrolyzed to produce monosaccharides, and glucose and xylose are the main six-carbon sugars and five-carbon sugars, respectively. In addition, incomplete saccharification in the saccharification stage will lead to more cellobiose in the saccharification solution. Efficient conversion of monosaccharides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/19C12N15/81C12R1/865
Inventor 汤岳琴木田建次苟敏孙照勇
Owner SICHUAN UNIV
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