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Method for preparing ethanol by using tobacco stems as biorefinery raw materials

A biorefining and raw material technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problems of low biotransformation efficiency and achieve high-efficiency biorefinery, cost saving, and production cost saving Effect

Inactive Publication Date: 2019-02-22
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, currently there are few studies on the use of tobacco rods for biorefining, and the bottleneck lies in the high resistance of lignocellulosic structures to cellulose enzymatic hydrolysis, resulting in extremely low bioconversion efficiency.

Method used

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  • Method for preparing ethanol by using tobacco stems as biorefinery raw materials
  • Method for preparing ethanol by using tobacco stems as biorefinery raw materials
  • Method for preparing ethanol by using tobacco stems as biorefinery raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for producing reducing sugar by biorefining of tobacco rods described in Example 1 is characterized in that the specific steps are as follows:

[0030] (1) Pretreatment of tobacco rod raw materials:

[0031] a. Take fresh tobacco rods and wash off the floating dust and impurities on the surface with natural water. After 10 days of natural drying outdoors, bake them in an oven at 50°C for 2 days, then take them out and cool them to room temperature, and grind them with a pulverizer. Chopped into small pieces of 0.5-5cm in length, put them into a 5L reactor, and add NaOH solution with a concentration of 4% according to the ratio of solid-to-liquid ratio of 1:5;

[0032] b. Soak the solid-liquid mixture in step (1) at a constant temperature of 40-75°C for 3 hours, then heat it to 80°C for pretreatment for 70 minutes, and transfer the heat-treated solid-liquid mixture to a suction filter for suction filtration After the suction filtration is completed, add distill...

Embodiment 2

[0036] Embodiment 2: a kind of pretreatment method that improves tobacco rod biotransformation efficiency, is characterized in that concrete steps are as follows:

[0037] (1) Pretreatment of tobacco rod raw materials:

[0038] a. Take fresh tobacco rods and wash off the floating dust and impurities on the surface with natural water. After 20 days of natural drying outdoors, bake them in an oven at 55°C for 2 days, then take them out and cool them to room temperature, and grind them with a pulverizer. Chopped into 0.5-5cm long pieces and put them into a 10L reactor, add 2% NaOH solution according to the solid-to-liquid ratio of 1:10;

[0039] b. Treat the solid-liquid mixture in step (1) in a water bath at 90°C for 60 minutes, wash with water and filter until neutral, collect the treated fibrous residue and dry it at 50-60°C to obtain the pretreated Tobacco rod raw material B.

[0040] (2) Add 10 g of Saccharomyces cerevisiae dry powder preparation into 120 mL of 3% glucose ...

Embodiment 3

[0043] Embodiment 3: a kind of pretreatment method that improves tobacco rod biotransformation efficiency, is characterized in that concrete steps are as follows:

[0044] (1) Pretreatment of tobacco rod raw materials:

[0045] a. First clean the fresh tobacco rods with natural water to remove the floating dust and impurities on the surface, and then dry them outdoors for 30 days, then bake them in an oven at 60°C for 1 day, then take them out and cool them to room temperature, and use a grinder to grind them It is chopped into small pieces with a length of 0.5 to 5 cm and put into an 8L reactor, and a NaOH solution with a concentration of 0.5% is added according to the ratio of solid to liquid ratio of 1:20;

[0046] b. Soak the solid-liquid mixture in step (1) at a constant temperature of 40°C for 0.5 hours, heat it to 90°C in a water bath for 60 minutes, and transfer the heat-treated solid-liquid mixture to a suction filter for suction filtration After the suction filtrati...

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Abstract

The invention provides a method for preparing ethanol by using tobacco stem biorefinery. A pretreatment method specifically includes the steps: selecting fresh tobacco stems with tobacco leaves removed; airing, drying and chopping the fresh tobacco stems; adding NaOH solution with the concentration of 0.2%-5%; placing the solution into a water bath at the temperature of 80-90 DEG C for treatment for 30-120 minutes; washing and filtering the solution to a neutral state; collecting treated fibrous residues; drying the residues to obtain pretreated tobacco stem raw materials and then adding cellulase accounting for 10-30% of the weight of the raw materials and adding sodium acetate buffer solution with the pH (potential of hydrogen) of 4.8 and the concentration of 50 mmol / L; inoculating activated yeast solution in a constant temperature water bath at the temperature of 40-50 DEG C for 48-72 hours, and collecting filtrate to obtain ethanol solution. According to the method, the yield of ethanol obtained by enzymatic saccharification and fermentation can reach 106.64 mg / g which is increased by 138% as compared with that of the untreated tobacco stems. The utilization rate of the tobaccostems is greatly increased.

Description

technical field [0001] The invention belongs to biorefining technology, in particular to a method for preparing ethanol by using tobacco rods as biorefining raw materials. Background technique [0002] Tobacco is one of the most important non-edible economic crops in the world and is widely grown in various countries. Tobacco leaves are the main raw material of the tobacco industry. After the tobacco leaves are harvested, a large amount of tobacco rods are often left. It is estimated that more than 260 million tons of tobacco rods are produced in the world every year in tobacco cultivation. These tobacco rods cannot be used as agricultural fuels, and they pollute the atmosphere after being burned. They can only be piled up in the fields to rot naturally, seriously polluting the environment. [0003] In recent years, environmentally friendly biorefinery technology has gradually become the development trend of resource utilization of lignocellulosic agricultural and forestry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCC12P7/10C12P19/14C12P2201/00Y02E50/10
Inventor 郭国宁宋旭艳李冉潘曦魏敏
Owner HUBEI CHINA TOBACCO IND
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