Method for preparing queen bee embryo peptide by enzyme hydrolysis method

A technology of enzymatic hydrolysis and queen bee, which is applied in compound screening, food science, material inspection products, etc., can solve the problems of no patent reports on hydrolyzed polypeptides, and achieve the effect of improving product utilization value, biological activity, and product quality

Active Publication Date: 2014-04-16
山东欣悦肽生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no patent reports on the technology of preparing hydrolyzed polypeptides using queen bee larvae, a protein resource with a wide range of sources and low prices.

Method used

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  • Method for preparing queen bee embryo peptide by enzyme hydrolysis method
  • Method for preparing queen bee embryo peptide by enzyme hydrolysis method
  • Method for preparing queen bee embryo peptide by enzyme hydrolysis method

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Embodiment Construction

[0038] The specific embodiments of the present invention will be described in further detail below in conjunction with the drawings and embodiments. The following examples are used to illustrate the present invention, but not to limit the scope of the present invention.

[0039] Step 1: Sample pretreatment: Collect 2 to 3 day old queen bee larvae (Queen bee embryos, 2 to 3 day old queen bee larvae have not grown and have very similar respiration rates. Later, as feeding conditions change, they begin to differentiate into queen worker bees. It is the basis for selecting the queen bee larvae for 2 to 3 days) appropriate amount, rapidly frozen at -80°C during the life of the queen bee larvae, freeze-dried with a freeze dryer, and ground the dried queen bee larvae sample into powder.

[0040] Step 2: Degreasing the queen bee larvae powder: put the pretreated queen bee larvae powder sample into figure 1 The supercritical extraction device shown is defatted, the queen bee larvae powder i...

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Abstract

The invention relates to a method for preparing queen bee embryo peptide by an enzyme hydrolysis method. The method comprises the steps of carrying out sample pretreatment, degreasing queen bee larva powder, preparing queen bee larva powder sample solution, treating the queen bee larva powder sample solution by ultrasonic waves, carrying out enzymatic hydrolysis reaction on the queen bee larva powder sample solution, heating enzymatic hydrolysate in a boiling water bath for 20min to realize enzyme deactivation, cooing to room temperature, adjusting pH value of the enzymatic hydrolysate to 7.0, centrifuging to take out supernate, and drying the supernate to obtain the hydrolyzed queen bee embryo powder. The prepared queen bee embryo polypeptide can be used for preparing health products for improving the function of human body, and more important, the bioactive polypeptides beneficial to human body can be screened out from the hydrolyzed queen bee embryo polypeptide.

Description

Technical field [0001] The invention relates to a method for preparing queen bee embryo peptide by enzymolysis. Background technique [0002] Queen larva, also known as queen fetus or royal fetus, is a larva that grows and develops into a larva from the fertilized eggs of bees fed with fresh royal jelly by worker bees. They are larvae taken from the surface of royal jelly in the royal terrace. The queen bee larvae feed on royal jelly. The larvae themselves have very rich nutrients, and at the same time, the royal jelly adheres to its body surface. It is a by-product of the artificial production of royal jelly. The queen bee larva is a living animal body, not only rich in nutrients, but also rich in enzymes and biologically active substances. Queen bee larvae not only have high protein content (accounting for more than 50% of dry matter), but also have a complete range of 18 amino acids, rich in 8 essential amino acids. The content of aspartic acid and glutamic acid is high, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06G01N21/31
CPCC12P21/06A23J3/34A23L33/18G01N33/50G01N2500/00
Inventor 赵树民石松传鲍勇刚
Owner 山东欣悦肽生物技术有限公司
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