Preparation method of momordica charantia p-polypeptide freeze-dried powder

A bitter gourd peptide and freeze-dried powder technology, applied in the field of biomedicine, can solve problems such as uncontrollable blood sugar, easy hypoglycemia, poor insulin absorption stability, etc., to improve grinding effect and production efficiency, reduce actual usage, enhance and The effect of improving flavor

Pending Publication Date: 2016-06-01
BEIHAI ASSI ROLLA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in reality, the blood sugar target rate of diabetic patients is low
Insulin injection is one of the methods to treat diabetes, but it has been found in clinical treatment that insulin injection itself has the following problems: (1) It can only reduce blood sugar during the injection period, and cannot completely control blood sugar according to the blood sugar change curve; (2) It is prone to hypoglycemia. Blood sugar; (3) The absorption stability of insulin is poor, and the absorption difference reaches 52%; (4) Complications cannot be effectively controlled
[0004] The preparation method of bitter melon polypeptide has been reported in the prior art. For example, the publication number CN101985644A published by the State Intellect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1

[0022] The preparation method of balsam pear polypeptide freeze-dried powder includes the steps of raw material pretreatment, degreasing, alkali dissolution, enzymatic hydrolysis, acid precipitation, centrifugation, washing, and freeze-drying. The enzyme used for enzymatic hydrolysis is papain with a mass ratio of 1.5:1 And flavor protease; the enzymolysis step also adds 1% of the total enzyme vitamin C.

Example Embodiment

[0023] Example 2

[0024] The preparation method of balsam pear polypeptide freeze-dried powder includes the steps of raw material pretreatment, degreasing, alkali dissolution, enzymatic hydrolysis, acid precipitation, centrifugation, washing, and freeze-drying. The enzyme used for enzymatic hydrolysis is papain with a mass ratio of 3:1 And flavor protease; the enzymolysis step also adds 6% of the total enzyme vitamin C.

[0025] In this example, the pretreatment of raw materials refers to removing the balsam pear seeds, selecting non-rotted kernels, drying the kernels in an oven for 20 minutes, grinding them, passing through a 40-mesh sieve, collecting the under-sieve and sterilizing them .

Example Embodiment

[0026] Example 3

[0027] The preparation method of balsam pear polypeptide freeze-dried powder includes the steps of raw material pretreatment, degreasing, alkali dissolution, enzymatic hydrolysis, acid precipitation, centrifugation, washing, and freeze-drying. The enzyme used for enzymatic hydrolysis is papain with a mass ratio of 2:1 And flavor protease; the enzymolysis step also adds 3% of the total enzyme vitamin C.

[0028] In this example, the pretreatment of raw materials refers to removing the balsam pear seeds, selecting non-rotted kernels, drying the kernels in an oven for 40 minutes, grinding them, passing through a 60-mesh sieve, collecting the under-sieve and sterilizing them ; Degreasing refers to adding acetone 10 times the weight of the bitter melon powder to the sterilized bitter gourd seed powder, stir it evenly, let it stand for 30 minutes, and then filter it.

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PUM

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Abstract

The invention discloses a preparation method of momordica charantia p-polypeptide freeze-dried powder and belongs to the technical field of biological medicines. The method comprises the following steps: raw material pretreatment, degreasing, alkali dissolution, enzymolysis, acid precipitation, centrifugation, washing, freezing and drying. An enzyme used for the enzymolysis contains a papaya protease and a flavor protease with a mass ratio of 1.5 to 3:1; vitamin C which is 1-6 percent of the total amount of the enzyme is also added in the enzymolysis. The preparation method disclosed by the invention has the advantages that the momordica charantia p-polypeptide purity is improved, and the momordica charantia p-polypeptide mouth feeling is improved.

Description

technical field [0001] The invention relates to a preparation method of balsam pear polypeptide freeze-dried powder, belonging to the technical field of biomedicine. Background technique [0002] Diabetes mellitus (DM) is a group of syndromes caused by environmental and genetic factors. Its basic pathological features are absolute or relative insufficiency of insulin secretion, or peripheral tissue insensitivity to insulin, mainly caused by glucose metabolism disorders, including A systemic disease characterized by disorders of fat and protein metabolism. Many evidence-based medical studies, such as the Clinical Trial of Diabetes Control and Complications (DCCT) and the UK Prospective Diabetes Study (UKPDS), have reached a consensus on the significance of intensified blood sugar control. Reaching the blood sugar target can not only prevent or delay chronic complications of diabetes The occurrence of diabetes will improve the quality of life of patients, and will greatly red...

Claims

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Application Information

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IPC IPC(8): A23J1/14
CPCA23J1/148A23V2002/00A23V2200/328
Inventor 卢泽芳
Owner BEIHAI ASSI ROLLA BIOTECH CO LTD
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