Preparation method of momordica charantia p-polypeptide freeze-dried powder

Abstract

The invention discloses a preparation method of momordica charantia p-polypeptide freeze-dried powder and belongs to the technical field of biological medicines. The method comprises the following steps: raw material pretreatment, degreasing, alkali dissolution, enzymolysis, acid precipitation, centrifugation, washing, freezing and drying. An enzyme used for the enzymolysis contains a papaya protease and a flavor protease with a mass ratio of 1.5 to 3:1; vitamin C which is 1-6 percent of the total amount of the enzyme is also added in the enzymolysis. The preparation method disclosed by the invention has the advantages that the momordica charantia p-polypeptide purity is improved, and the momordica charantia p-polypeptide mouth feeling is improved.

Description

technical field [0001] The invention relates to a preparation method of balsam pear polypeptide freeze-dried powder, belonging to the technical field of biomedicine. Background technique [0002] Diabetes mellitus (DM) is a group of syndromes caused by environmental and genetic factors. Its basic pathological features are absolute or relative insufficiency of insulin secretion, or peripheral tissue insensitivity to insulin, mainly caused by glucose metabolism disorders, including A systemic disease characterized by disorders of fat and protein metabolism. Many evidence-based medical studies, such as the Clinical Trial of Diabetes Control and Complications (DCCT) and the UK Prospective Diabetes Study (UKPDS), have reached a consensus on the significance of intensified blood sugar control. Reaching the blood sugar target can not only prevent or delay chronic complications of diabetes The occurrence of diabetes will improve the quality of life of patients, and will greatly red...

AI Technical Summary

Problems solved by technology

However, in reality, the blood sugar target rate of diabetic patients is low

Insulin injection is one of the methods to treat diabetes, but it has been found in clinical treatment that insulin injection itself has the following problems: (1) It can only reduce blood sugar during the injection period, and cannot completely control blood sugar according to the blood sugar change curve; (2) It is prone to hypoglycemia. Blood sugar; (3) The absorption stability of insulin is poor, and the absorption difference reaches 52%; (4) Complications cannot be effectively controlled

[0004] The preparation method of bitter melon polypeptide has been reported in the prior art. For example, the publication number CN101985644A published by the State Intellectual Property Office on March 16, 2011, the invention patent titled "A kind of bitter melon polypeptide and its preparation method and application", This patent uses papain to directly enzymatically hydrolyze bitter melon powder, and then settles, filters, and collects the filtrate to achieve the purpose of extraction. The raw material used in this method is bitter melon powder, which includes bitter melon seeds and bitter melon meat, but the bitter melon polypeptide exists in In bitter gourd seeds, crushing bitter gourd meat and bitter gourd seeds together to extract polypeptides will greatly increase the cost, and the product has high impurity content, low purity of bitter gourd polypeptides, and the corresponding use effect is also poor