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A method for preparing queen bee embryo peptide by enzymatic hydrolysis

An enzymatic hydrolysis method and queen bee technology, which is applied in the fields of compound screening, food science, material testing, etc., can solve the problem of no patent report on hydrolyzed polypeptides, and achieve the effect of improving product utilization value, improving taste and reducing cost.

Active Publication Date: 2016-07-06
山东欣悦肽生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no patent reports on the technology of preparing hydrolyzed polypeptides using queen bee larvae, a protein resource with a wide range of sources and low prices.

Method used

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  • A method for preparing queen bee embryo peptide by enzymatic hydrolysis
  • A method for preparing queen bee embryo peptide by enzymatic hydrolysis
  • A method for preparing queen bee embryo peptide by enzymatic hydrolysis

Examples

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Embodiment Construction

[0038] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0039] Step 1: Sample pretreatment: collect 2-3 day-old queen bee larvae (queen bee embryos, 2-3 day-old queen bee larvae have not grown and have a similar respiration rate, and they begin to differentiate into queen bee worker bees as feeding conditions change later. It is the basis for selecting queen bee larvae for 2 to 3 days) appropriate amount, freeze rapidly at -80°C during the survival period of queen bee larvae, freeze-dry with a freeze dryer, and grind the dried queen bee larvae sample into powder.

[0040] Step 2: Queen bee larvae powder defatting: Put the pretreated sample of queen bee larvae powder into such as figure 1 Degrease the supercritical extraction d...

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PUM

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Abstract

Provided is a method of preparing queen bee embryo peptides by enzymolysis, comprising: pretreating a sample, removing lipids from the queen bee larvae powder, preparing queen bee larvae powder sample liquid, conducting ultrasonic treatment, conducting enzymolysis, placing the enzymatic hydrolysates into boiling water for 20 minutes to deactivate the enzyme, cooling to room temperature, adjusting the PH value of the enzymatic hydrolysates to 7.0, removing the supernatant after centrifuging, obtaining hydrolyzed queen bee embryo peptide powder after drying. The queen bee embryo polypeptides of the invention can be used to produce health care products for improving functions of the human body, and screen biologically active polypeptides effective for the human body.

Description

technical field [0001] The invention relates to a method for preparing queen bee embryo peptide by enzymatic hydrolysis. Background technique [0002] Queen bee larvae, also known as queen bee larvae or queen bee larvae, are fertilized eggs of bees that grow and develop into larvae after being fed fresh royal jelly by worker bees. Queen bee larvae feed on royal jelly. The larvae themselves are rich in nutrition, and royal jelly adheres to their body surface. It is a by-product of the artificial production of royal jelly. The queen bee larva is a living animal body, not only rich in nutrients, but also rich in enzymes and biologically active substances. The queen bee larva not only has high protein content (accounting for more than 50% of dry matter), but also has 18 kinds of amino acids and is rich in 8 kinds of essential amino acids for human body. The content of aspartic acid and glutamic acid is relatively high, and these two amino acids have a good brain-building effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06G01N21/31
CPCC12P21/06A23J3/34A23L33/18G01N33/50G01N2500/00
Inventor 赵树民石松传鲍勇刚
Owner 山东欣悦肽生物技术有限公司
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