Making method of high-organic selenium cardamine violifolia selenium polypeptide

Abstract

The invention relates to a making method of a high-organic selenium cardamine violifolia selenium polypeptide. The making method comprises the following steps of: S1, performing pretreatment on a rawmaterial: selecting dried cardamine violifolia powder as a raw material for production, performing soaking with ethano for decolorization, performing filtering for removing impurities, and then performing pretreatment with a mixture of cellulolytic enzymes and pectinase to obtain an enzymatic hydrolysate for standby application; S2, performing double-enzyme composite enzymolysis: firstly adding alkaline protease in a supernatant after pretreatment in step S1 for enzymolysis, performing enzyme deactivation, performing cooling to 50 DEG C, adjusting the pH with hydrochloric acid to 6.0-8.0, adding neutral protease, and performing enzymolysis to obtain an enzymatic hydrolysate; and S3, performing enzyme deactivation and peptide solution separation: boiling over the enzymatic hydrolysate to achieve enzyme deactivation, and performing standing to obtain a clear transparent peptide solution in the lower layer. The making method disclosed by the invention has the advantages that the cardamineviolifolia is used a core raw material, and the cellulolytic enzymes and the pectinase are used for pretreatment before enzymolysis, so that the impurities in the cardamine violifolia are effectivelyremoved and the protein extraction rate of the cardamine violifolia is increased; and then a multi-step double-enzyme enzymolysis method is used for enzymolysis, the content of the selenium polypeptide is higher, and the molecular weight of the selenium polypeptide is smaller.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for preparing a high-organic selenium-leaved selenopolypeptide. Background technique [0002] Selenium is an essential trace element for the human body. It has various functions such as anti-oxidation, delaying aging, improving immunity, preventing cancer and fighting cancer. At present, it has been confirmed that selenium deficiency is closely related to the occurrence of Keshan disease and Kaschin-Beck disease. In addition, a large number of epidemiological studies at home and abroad have found that selenium deficiency is potentially related to cardiovascular disease, tumor development and liver disease. The form of selenium is often divided into organic selenium and inorganic selenium. Inorganic selenium includes selenite, selenate, selenide, and elemental selenium, and organic selenium includes selenomethionine, selenocystine, and selenocysteine, yeast selenium...

AI Technical Summary

Problems solved by technology

At present, most of the selenium supplements on the market are based on inorganic selenium. Exceeding a certain dose of inorganic selenium will cause obvious toxicity to the body, resulting in hair and nail loss and liver and kidney toxicity.

The second-generation selenium supplementation preparation, organic selenium mainly composed of yeast selenium, has significantly lower toxicity than inorganic selenium, but because its main component is selenomethionine, which contains high purine, long-term use has a potential risk of gout

As a new generation of selenium supplement products, plant selenoprotein has obvious safety advantages and functional effects. However, as a biological macromolecule, it needs to be digested in the body to be absorbed, so its bioavailability is limited.

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