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Food grade phycocyanin and preparation method thereof

A phycocyanin and food-grade technology, which is applied in the field of separation and purification of phycocyanin, can solve the problems of restricting the promotion and use of spirulina phycocyanin products, expensive phycocyanin, and incapable of large-scale preparation, so as to achieve low production cost, The effect of low production cost and low equipment requirements

Active Publication Date: 2013-07-24
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, phycocyanin can be extracted from several species of seaweed, but high-purity phycocyanin is very expensive and the price is increasing rapidly
[0005] The traditional preparation method of spirulina phycocyanin generally includes repeated freezing and thawing, refrigerated centrifugation, salting out by sulfuric acid, dialysis, column desalting, multiple gradient elution, freeze drying, etc., the process is cumbersome, the yield is low, the cost is high, and the price Expensive, and cannot be prepared in large quantities, which severely limits the popularization and use of spirulina phycocyanin products

Method used

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  • Food grade phycocyanin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation method steps of food grade phycocyanin are as follows:

[0029] (1) Take 5 kg of spirulina powder, put the prepared 4°C equivalent phosphate buffer into the mixing granulator, adjust the time relay, set the wet mixing time to 3 minutes, and turn on the stirring motor and the chopping motor , set the stirring blade speed as I speed, and the chopping knife speed as II speed for wet mixing. After the wet mixing is completed, open the discharge piston, turn on the stirring paddle, and discharge the material. Use a 16-mesh nylon mesh oscillating granulator to make spirulina wet granules, and then use a colloid mill to grind the spirulina wet granules circularly and pulverize them fully. During grinding, the temperature does not exceed 4 °C.

[0030] (2) Add 25 liters of 0.01-0.05M, pH6.0-7.0 phosphate buffer solution to the spirulina powder ground by the colloid mill, and place it in a freezer for extraction for more than 8 hours;

[0031] (3) The leaching ...

Embodiment 2

[0037] The preparation method steps of food grade phycocyanin are as follows:

[0038] (1) Take 10 kg of spirulina powder, put the prepared 4°C equivalent phosphate buffer into the mixing granulator, adjust the time relay, set the wet mixing time to 3 minutes, and turn on the stirring motor and the chopping motor , set the stirring blade speed as I speed, and the chopping knife speed as II speed for wet mixing. After the wet mixing is completed, open the discharge piston, turn on the stirring paddle, discharge the material, use a 16-mesh nylon mesh oscillating granulator to make wet spirulina granules, and then grind the wet spirulina granules circularly with a colloid mill to fully pulverize. During grinding, the temperature does not exceed 4 °C.

[0039] (2) Add 50 liters of 0.01-0.05M, pH6.0-7.0 phosphate buffer solution to the spirulina powder ground by the colloid mill, and place it in a freezer for extraction for more than 8 hours;

[0040] (3) The leaching solution is...

Embodiment 3

[0046] The steps of the food grade phycocyanin preparation method are as follows:

[0047] (1) Take 15 kg of spirulina powder, put the prepared 4°C equivalent phosphate buffer into the mixing granulator, adjust the time relay, set the wet mixing time to 3 minutes, and turn on the stirring motor and the chopping motor , set the stirring blade speed as I speed, and the chopping knife speed as II speed for wet mixing. After the wet mixing is completed, open the discharge piston, turn on the stirring paddle, and discharge the material. Use a 5-mesh nylon mesh oscillating granulator to make spirulina wet granules, and then use a colloid mill to grind the spirulina wet granules circularly and fully pulverize. During grinding, the temperature does not exceed 4 °C.

[0048] (2) Add 75 liters of 0.01-0.05M, pH6.0-7.0 phosphate buffer solution to the spirulina powder ground by the colloid mill, and place it in a freezer for extraction for more than 8 hours;

[0049] (3) The leaching s...

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Abstract

The invention discloses a preparation method of food grade phycocyanin protein, comprising the following steps: breaking the wall of the spirulina power; adding weak acid salt buffer solution in the wall broken spirulina power for digestion; carrying out solid-liquid separation on the spirulina power leach liquor; extracting supernatant; filtering the supernatant to obtain the crude extract; and purifying the crude extract to obtain the concentrated solution; and spraying and drying the concentrated solution. The invention also discloses a food grade phycocyanin protein prepared by the method, wherein the purity (OD620 / OD280) is larger than 1.0; the content of the phycocyanin protein is larger than 50%; the content of allophycocyanin is larger than 10%; the content of phycoerythrin is larger than 2%; and the content of other proteins is smaller than 2%. The method has low production cost, is simple and effective, and is suitable for preparing mass food grade phycocyanin proteins.

Description

technical field [0001] The invention belongs to the technical field of biochemical separation and purification, in particular to a method for separation and purification of phycocyanin. Background technique [0002] Spirulina is the cyanobacteria in microalgae, it has the characteristics of high protein, low fat, low sugar, low cholesterol, contains a variety of vitamins, proteins, fats, carbohydrates, minerals and trace elements, and various proportions are scientific and reasonable, of which Many ingredients reach the content and strength of the drug, which makes it have a wide range of medicinal functions. It can promote human metabolism, improve the body's immunity, improve physical fitness, resist radiation, assist cancer radiotherapy and chemotherapy, and reduce the side effects of radiotherapy and chemotherapy. It has positive effects and is an ideal pure natural bioactive health food. [0003] Phycobiliprotein is a binding (conjugated) protein formed by covalently l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/405C07K1/34C07K1/14
Inventor 秦松刘冰闫鸣艳林甜甜衣悦涛
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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