A kind of preparation method of Selenium Polypeptide of Viola Leaf Broken Camelina with High Organic Selenium Content

Abstract

A method for preparing a Selenium Polypeptide of Viola Leaf Broken Camelina with high organic selenium content, comprising the following steps: S1, raw material pretreatment: select the dry powder of Broken Camelina as a production raw material, soak it in ethanol for decolorization, filter, remove impurities, and then pass through fiber Obtain enzymatic hydrolysis products after pretreatment with the mixture of sudase and pectinase, and set aside; S2, double-enzyme compound enzymolysis: add alkaline protease to the supernatant after step S1 pretreatment for enzymolysis, inactivate the enzyme, and cool to 50°C, adjust the pH to 50°C with hydrochloric acid, add neutral protease, and enzymatically hydrolyze to obtain an enzymatic hydrolyzate; S3, inactivate enzyme, separate peptide liquid: boil the enzymolyzate to inactivate the enzyme, let stand, and the lower layer will be clear Transparent peptide fluid. Its advantages are: the core raw material is Viola leaf broken Camelina, and cellulase and pectinase are used for pretreatment before enzymatic hydrolysis, effectively removing impurities in the broken Camelina, and improving the protein extraction rate of the Broken Camelina; Enzyme step-by-step enzymatic hydrolysis, the content of selenium polypeptide is relatively high, and the molecular weight is small.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for preparing a high-organic selenium-leaved selenopolypeptide. Background technique [0002] Selenium is an essential trace element for the human body. It has various functions such as anti-oxidation, delaying aging, improving immunity, preventing cancer and fighting cancer. At present, it has been confirmed that selenium deficiency is closely related to the occurrence of Keshan disease and Kaschin-Beck disease. In addition, a large number of epidemiological studies at home and abroad have found that selenium deficiency is potentially related to cardiovascular disease, tumor development and liver disease. The form of selenium is often divided into organic selenium and inorganic selenium. Inorganic selenium includes selenite, selenate, selenide, and elemental selenium, and organic selenium includes selenomethionine, selenocystine, and selenocysteine, yeast selenium...

AI Technical Summary

Problems solved by technology

At present, most of the selenium supplements on the market are based on inorganic selenium. Exceeding a certain dose of inorganic selenium will cause obvious toxicity to the body, resulting in hair and nail loss and liver and kidney toxicity.

The second-generation selenium supplementation preparation, organic selenium mainly composed of yeast selenium, has significantly lower toxicity than inorganic selenium, but because its main component is selenomethionine, which contains high purine, long-term use has a potential risk of gout

As a new generation of selenium supplement products, plant selenoprotein has obvious safety advantages and functional effects. However, as a biological macromolecule, it needs to be digested in the body to be absorbed, so its bioavailability is limited.

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