Biological sandstone preparation method and device based on MICP (Microbial Induced Carbonate Precipitation) technology

A biological and sandstone technology, applied in the direction of fertilization devices, chemical instruments and methods, soil protection, etc., can solve the problems of reducing the utilization rate of urea and mineral calcium salts, increasing construction difficulty and construction cost, so as to promote practical application and Promote, reduce construction difficulty and cost, and improve the effect of defects

Inactive Publication Date: 2016-02-03
INNER MONGOLIA UNIV OF TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006] Although the above methods have achieved some results, there are still some defects: when the pumping method is used, most samples are prepared in a cylindrical shape, which can make the nutrient solution penetrate into the pores of the sample better under a certain pressure, but the lower end The outlet will take away part of the bacteria and urease, while reducing the utilization rate of urea and mineral calcium salt, and the method of injecting the solution in batches will increase the construction difficulty and construction cost in actual engineering

Method used

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  • Biological sandstone preparation method and device based on MICP (Microbial Induced Carbonate Precipitation) technology
  • Biological sandstone preparation method and device based on MICP (Microbial Induced Carbonate Precipitation) technology
  • Biological sandstone preparation method and device based on MICP (Microbial Induced Carbonate Precipitation) technology

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Experimental program
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Embodiment 1

[0042] Embodiment 1: Fine-grained biological sandstone is made

[0043] Mold is cylindrical, and the diameter d=50mm of mold, height h=50mm, place particle diameter 0.5-1mm standard sand 47.3g in each mold, and particle diameter is 0.25mm-0.1mm natural desert sand (fine sand) 110g, Median particle size D of mixed sand 50 is 0.22. Mix 40ml of bacteria solution and growth medium, prepare 10 samples, put them on the plexiglass bracket, immerse in 20L cementing nutrient solution, turn on the air pump and magnetic stirrer, after 7 days of MICP curing, demould, take out Formed coarse-grained biological sandstone.

Embodiment 2

[0044] Embodiment 2: making of coarse-grained biological sandstone

[0045] The diameter of the mold is d=50mm, and the height is h=50mm. In each mold, 139.7g of standard sand with a particle size of 0.5mm-1mm and 15.5g of natural desert sand (fine sand) with a particle size of 0.25mm-0.1mm are placed in each mold. , the median particle size D of the mixed sand 50 is 0.87. Mix 40ml of bacteria and growth medium, prepare 10 samples, put them on the brackets, immerse in 20L cementing solution, turn on the air pump and magnetic stirrer, take it out after 7 days of experiment, take off the mold, and take out the formed coarse-grained organisms sandstone.

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Abstract

The invention discloses a biological sandstone preparation method and device based on an MICP (Microbial Induced Carbonate Precipitation) technology. The preparation method comprises the steps as follows: step A: mixed sand, a bacterium growth media and a cementing nutrient solution are prepared; step B: the mixed sand, a bacteria solution and the growth media are mixed in proportion and put into a mold; and step C: the mold is placed on a support and immersed into the cementing nutrient solution for a reaction, the cementing nutrient solution contains urea, mineral calcium salt and a binder, and after the full reaction, the mold is removed, so that biological sandstones are obtained. According to the device and the method, the prepared biological sandstones are excellent in uniformity, high in strength and environmental-friendly; and the device is easy to use, so that the construction difficulty and the cost are reduced in practical engineering application, the practicability of the technology is improved, and practical application and popularization of the biological sandstones prepared by the MICP (Microbial Induced Carbonate Precipitation) technology are facilitated.

Description

technical field [0001] The present invention relates to microbial induction of CaCO 3 Precipitation (Microbial Induced Carbonate Precipitation, referred to as MICP) technology, in particular, relates to a preparation method and device for biological sandstone based on MICP technology. Background technique [0002] With the worldwide population growth and social development, the demand for construction land continues to increase. Traditional soil improvement methods often add chemical grouting materials and chemical curing agents for grouting to strengthen the soil, causing adverse effects on the environment. There are some specific microorganisms in nature, such as Bacillus Pasteurella, Myxococcus xanthus, and some denitrifying microorganisms, which use the surrounding organic matter such as urea and calcium ion sources to induce the formation of Calcium carbonate, this process is called microbial induced calcium carbonate mineralization (MicrobialInducedCarbonatePrecipita...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E02D3/12C09K17/00C09K103/00
Inventor 李驰李琳高瑜刘世慧斯日古楞
Owner INNER MONGOLIA UNIV OF TECH
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