Biological agent for organic waste composting and preparation method thereof
A technology of organic waste and biological bacteria agent, applied in the field of microorganisms, can solve problems such as accumulation, soil pollution, and harm to human health, and achieve the effects of enhancing composting efficiency, increasing fertilizer efficiency, and increasing C:N ratio
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Embodiment 1
[0042] (1) Expansion of strains
[0043] A. Cultivation of lactic acid bacteria: Place the original strain of Lactobacillus delbrueckii on the wort medium, do the primary slope culture at 28°C, then inoculate it into the same liquid wort medium for shaking secondary culture, the temperature is 28°C, 120r / min, cultured for 36-72h to obtain the secondary culture of lactic acid bacteria;
[0044] Cultivation of yeast B: Place the original strain of Torulopsis sphaeroides on lactic acid-potato-glucose medium, do primary slant culture at 28°C, and then inoculate into liquid medium with the same composition for shaking secondary culture, temperature 28°C, 120r / min, cultured for 36-72h to obtain a secondary culture of yeast;
[0045] C Culture of nitrifying bacteria: Nitrifying bacteria include nitrosobacteria and nitrifying bacteria, which convert ammonia nitrogen in water into nitrite and nitrate respectively. They are cultured on a slope at 25°C, and then inoculated into a triang...
Embodiment 2
[0054] (1) Expansion of strains
[0055] Culture method is the same as embodiment 1, and lactic acid bacteria is selected lactis streptococcus for use, and saccharomycete is selected grape juice yeast for use, and nitrifying bacteria is nitrifying bacteria and nitrosifying bacteria, and actinomycetes selects Actinomyces naeserii for use, and mold selects Aspergillus niger for use.
[0056] Viable count, detection of Streptococcus lactis, grape juice yeast, nitrifying bacteria, nitrosifying bacteria, Actinomyces neeseri, Aspergillus niger, mixed according to weight fraction: 40 parts of lactis streptococcus, 30 parts of grape juice yeast, 13 parts of Nitrobacter, 2 parts of Nitrosobacter, 15 parts of Actinomyces naesui and 15 parts of Aspergillus niger.
[0057] (2) Preparation of biological agents
[0058] Add 50 parts by weight of wheat bran, 30 parts of wheat husk, 20 parts of sawdust and 20 parts of bamboo charcoal granules in a solid fermenter to a total of 8000g, sterili...
Embodiment 3
[0060] (1) Expansion of strains
[0061] The culture method is the same as that in Example 1. The lactic acid bacteria is selected from Bifidobacterium, the saccharomyces is selected from Hansenula, the nitrifying bacteria are nitrifying bacteria and nitrosifying bacteria, the actinomycetes are selected from Actinomyces mucilage, and the mold is selected from Penicillium citrinum.
[0062] Viable bacteria count, detection of bifidobacterium, Hansenula, nitrifying bacteria, nitrosifying bacteria, mucilaginous actinomycetes, Penicillium citrinum, mix them according to weight fraction: 35 parts of Bifidobacterium, 25 parts of Hansenula, 5 parts of Nitrobacter, 10 parts of Nitrosobacter, 12 parts of Actinomyces slime and 14 parts of Penicillium citrinum.
[0063] (2) Preparation of biological agents
[0064] Add 40 parts by weight of wheat bran, 25 parts of wheat husk, 15 parts of sawdust and 15 parts of bamboo charcoal granules in a solid fermenter to a total of 12000g, steriliz...
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