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Straw compost after-ripening enzyme accelerator and use method therefor

A technology of enzyme accelerator and straw, which is applied in the field of straw composting enzyme accelerator, can solve the problems that the decomposing process is difficult to work, endangers human health, and the effect is not obvious enough, so as to improve the germination rate of seeds, promote crop growth, and reduce biological toxic effect

Inactive Publication Date: 2015-10-21
NANJING UNIV OF INFORMATION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although in recent years, there have been many studies on microbial agents that promote compost maturity, but the ingredients are often single and the effect is not obvious enough.
In addition, most of the existing research is to inoculate microbial agents or other accelerators at the initial stage of composting. After entering the high-temperature stage, the growth of the flora is affected and it is difficult to survive. Therefore, it is difficult to work on the compost decomposition process after the high-temperature stage. Post-ripening accelerators are of great significance for promoting the maturity of straw compost
In the past, although there were a few studies on post-ripening preparations for straw composting, a variety of materials and chemical reagents were often used. These chemical reagents can often endanger human health through inhalation and skin contact, and are also potentially harmful to the soil environment and have certain biological toxicity. and environmental risks; and the materials used are more and more complex

Method used

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  • Straw compost after-ripening enzyme accelerator and use method therefor
  • Straw compost after-ripening enzyme accelerator and use method therefor
  • Straw compost after-ripening enzyme accelerator and use method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The cellulase used is cellulase produced by Trichoderma, origin: Beijing. Carboxymethylcellulase activity is 1,034 U g -1 . The optimum working temperature is 40-50 C. The protease used is AS1.398 neutral protease ( Bacillus subtilis neutral protease). The exogenous protease activity is 58,820 U g -1 . The optimum temperature for exogenous protease is 35-45 C.

[0025] The straw used is rice straw, which comes from the paddy fields of Wujing Town, Minhang District, Shanghai.

[0026] Composting:

[0027] (1) Two groups of straw compost were set up, one group was the enzyme-added compost to be added with post-ripening enzyme accelerator, and the other was the control compost without adding post-ripening enzyme accelerator. Each group of straw composting was firstly subjected to conventional composting treatment: the straw was cut into small pieces less than 5 cm, mixed according to the ratio of 150 kg of straw and 300 kg of water, stacked outdoors, and covered wi...

Embodiment 2

[0041] The cellulase used is the cellulase produced by Trichoderma. Carboxymethylcellulase activity at 1,000 U g -1 , the optimum temperature is 40-50 C. The protease used is AS1.398 neutral protease ( Bacillus subtilis neutral protease). The exogenous protease activity is 50000 U g -1 . The optimum temperature for exogenous protease is 35-45 C.

[0042] The straw used is wheat straw, which comes from the wheat field in Pancheng Town, Pukou District, Nanjing.

[0043] Composting:

[0044] (1) Two groups of straw compost were set up, one group was the enzyme-added compost to be added with post-ripening enzyme accelerator, and the other was the control compost without adding post-ripening enzyme accelerator. Each group of straw composting was firstly subjected to conventional composting treatment: the wheat straw was crushed into small pieces less than 5 cm, mixed according to the ratio of 100 kg of straw and 200 kg of water (moisture content about 67%), stacked outdoors,...

Embodiment 3

[0056] The cellulase used is the cellulase produced by Trichoderma. Carboxymethylcellulase activity at 1,000 U g -1 , the optimum temperature is 40-50 C. The test protease is AS1.398 neutral protease ( Bacillus subtilis neutral protease). The exogenous protease activity is 50000 U g -1 . The optimum temperature for exogenous protease is 35-45 C.

[0057] The straw used in the test was corn straw, which came from the wheat-corn rotation area of ​​Bincheng District, Binzhou City.

[0058] Chinese cabbage ( Brassica chinensis L. ) seeds (variety: Shanghai Qing; place of origin: Shanghai).

[0059] Composting:

[0060] (1) Two groups of straw compost were set up, one group was the enzyme-added compost to be added with post-ripening enzyme accelerator, and the other was the control compost without adding post-ripening enzyme accelerator. Each group of straw composting is firstly subjected to conventional composting treatment: crush corn straw into small pieces of about 2 ...

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Abstract

The invention discloses a straw compost after-ripening enzyme accelerator and a use method therefor. The enzyme accelerator consists of trichoderma produced cellulase and neutral protease AS1.398, wherein the content of the trichoderma produced cellulase is 40-45%. The use method for the enzyme accelerator comprises the steps of chopping straws into small segments with length less than 5cm, carrying out conventional composting on the small segments, sprinkling accelerator enzyme liquid, which is prepared according to the blending ratio of 1: (8-12), to straw compost when the temperature of the straw compost is dropped below 50 DEG C, enabling the enzyme liquid and the straw compost to be uniformly mixed in a layered adding or turning composting manner, and carrying out standing until the straw compost is well-composted. According to the enzyme accelerator, the time for composting is shortened by about 15 days, the content of nutrients in soil is increased, and the growth of plants is promoted; and meanwhile, the use process is safe, non-toxic and harmless, so that the guarantee of food safety and soil environment is facilitated.

Description

technical field [0001] The invention relates to the technical field of straw composting treatment, in particular to a post-ripening enzyme accelerator for straw composting and a use method thereof. Background technique [0002] my country is rich in straw resources, and direct burning has accelerated soil degradation, resulting in the lack of organic matter compensation in the soil and land failure, and the soil ecological environment has fallen into a vicious circle. The pollution of chemical fertilizers to the environment is becoming more and more serious, resulting in soil compaction, biomass reduction, and soil nutrition becoming increasingly simplistic. Crop straw has a high content of organic matter and contains various nutrients needed by crops and microorganisms. The development and utilization of straw resources can maintain soil fertility. Composting is an important way to solve the recycling of agricultural waste, but there are problems such as slow decomposition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05F17/00
CPCY02W30/40
Inventor 韩玮何明聂俊华
Owner NANJING UNIV OF INFORMATION SCI & TECH
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