Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof

A yeast strain and xylose technology, applied in the field of bioengineering, can solve the problems of excessive accumulation of by-product xylitol, slow utilization of xylose, and inability to meet production requirements

Active Publication Date: 2013-04-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, my country has made some progress in the construction of Saccharomyces cerevisiae engineering strains that can use xylose to produce ethanol using metabolic engineering methods, but it still cannot meet the production requirements, mainly due to the slow utilization of xylose and excessive accumulation of by-product xylitol. Many and low yield of ethanol

Method used

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  • Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof
  • Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof
  • Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Construction and Screening of Recombinant Yeast Strain SyBE005

[0022] Departure strain: Departure strain L2612 was donated by Professor Thomas Jeffries of the University of Wisconsin-Madison. The genotype is MAT alpha, leu2, ura3, trp1. It is deficient in leucine, uracil and tryptophan.

[0023] culture medium

[0024] Screening medium A: synthetic nitrogen source YNB 6.7g / L, mixed amino acid powder 2g / L (refer to [US] D.C. Amberg et al. Yeast Genetics Method Test Guide for specific formula), leucine 100mg / L, tryptophan 100mg / L, glucose 20g / L. Screening medium B: synthetic nitrogen source YNB 6.7g / L, mixed amino acid powder 2g / L, tryptophan 100mg / L, glucose 20g / L. Screening medium C: synthetic nitrogen source YNB 6.7g / L, mixed amino acid powder 2g / L, glucose 20g / L. Screening medium D: synthetic nitrogen source YNB 6.7g / L, mixed amino acid powder 2g / L, aureobasicin A 0.5mg / L, glucose 20g / L. Acclimatization medium: synthetic nitrogen source YNB 6.7g / L, mixed amino ...

Embodiment 2

[0037] Example 2 Comparison of Xylose Fermentation of Bacterial Strains L2612PR, L2612PR-DPPP and SyBE005

[0038]1. Test materials: strains L2612PR, L2612PR-DPPP, SyBE003

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Abstract

The invention discloses a recombinant yeast strain (Saccharomyces cerevisiae)) capable of efficiently metabolizing xylose, which is named SyBE005. The recombinant yeast strain has been collected in Common Microorganism Center of China Committee for Culture Collection of Microorganisms, the collection number is CGMCC No.6634, and the recombinant yeast strain has the capacity of efficiently metabolizing xylose to produce ethanol. The recombinant yeast strain SyBE005 can effectively ferment xylose to generate ethanol. The ethanol yield reaches 64% of the theoretical yield, and the yield of the byproduct xylitol is lower than 12%; and thus, the recombinant yeast strain can efficiently convert xylose into ethanol, and is an excellent strain utilizing cellulose hydrolysate.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a recombinant yeast strain capable of fermenting xylose to produce ethanol. Background technique [0002] Utilizing lignocellulose to produce fuel ethanol can not only reduce the production cost of fuel ethanol, but also play an important role in improving the environment and waste disposal. As a large agricultural country, our country is rich in crop waste resources such as straw, which provides unique conditions for the development of fuel ethanol industry. Saccharomyces cerevisiae is generally used for industrial production of ethanol. Although natural Saccharomyces cerevisiae can use the hexose sugar in lignocellulose hydrolyzate to produce ethanol, it lacks the ability to metabolize abundant pentose sugars, such as xylose. . Therefore, the construction of engineering strains that can efficiently utilize xylose is of key significance for reducing the production cost ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12P7/06C12R1/865
CPCY02E50/10
Inventor 元英进査健申明华胡梦龙
Owner TIANJIN UNIV
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