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Fermentable silk spore yeast strains and application for preparing microbial oil thereof

A technology of microbial oils and yeast strains, applied in the fields of fermentation engineering and microbial engineering, can solve problems such as black smoke polluting the environment

Inactive Publication Date: 2011-02-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, this part of lignocellulosic resources was often discarded or used in a low-value manner, such as crop straws were incinerated and used for composting in farmland, but the black smoke generated during the incineration process will pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Obtaining of Trichosporon fermentans strain HWZ004

[0024] A series of mutagenized Trichosporon fermentans strains were obtained through multiple ultraviolet mutagenesis and combined ultraviolet and LiCl mutagenesis, among which the optimal strain was selected and named Trichosporon fermentans HWZ003, allowing it to grow in untreated Acclimate in any adsorbed and detoxified rice or bagasse acid hydrolyzate to obtain a target strain that can efficiently accumulate oil in lignocellulose hydrolyzate, named Trichospora fermentans HWZ004, the specific mutagenesis and domestication process includes the following Method and steps:

[0025] 1) Ultraviolet mutagenesis: fermentative Trichosporon fermentans CICC1368 (purchased from China Industrial Microorganism Culture Collection Center) in the activation medium (glucose 20g / L, yeast extract 10g / L, peptone 10g / L) Dilute 10 after culturing for 24-30 hours 5 times, spread on solid activation medium (glucose 20g / L; peptone 10g / L;...

Embodiment 2

[0031] The application of strain CCTCC M209060 to efficiently use the acid hydrolyzate of rice straw detoxified by activated carbon to ferment and produce microbial oil:

[0032] The preparation of rice straw hydrolyzate: rice straw is pulverized to 20 orders, weighs 40g, according to the solid-liquid ratio of rice straw (solid) and 2% (weight) dilute sulfuric acid (liquid) is 1g: 20mL adds dilute sulfuric acid solution, in It was hydrolyzed at 121°C for 1 hour, and the acid hydrolyzate of rice straw was obtained after filtration.

[0033] Detoxification pretreatment of rice straw hydrolyzate: adding Ca(OH) to rice straw acid hydrolyzate 2 Powder, adjust its pH to 10.0, keep it for 1h, then filter. Adjust the pH of the filtrate to 5.5 with HCl, let it stand for 1 h, and then centrifuge for 10 min to remove the precipitate. Activated carbon (ca. 2.5 mm, Merck, Germany) was added to the obtained hydrolyzate according to the solid-to-liquid ratio of activated carbon to hydrolyz...

Embodiment 3

[0038] The application of the strain CCTCC M209060 to efficiently utilize the acid hydrolyzate of rice straw that has not been detoxified by activated carbon for fermentation to produce microbial oil:

[0039] The difference between this embodiment and embodiment 2 is:

[0040] The lignocellulose hydrolyzate used in the fermentation medium has not undergone any adsorption and detoxification by activated carbon or other substances, and the other components in the medium are exactly the same as those in Example 2.

[0041] Pick 3 rings of fermentative Trichosporium CCTCC M209060 on the slope and inoculate them into YEPD medium (glucose 20g / L; peptone 10g / L; yeast powder 10g / L) for activation, and transfer to 10% inoculum after 24 hours. Connect to 50mL of the fermentation medium prepared above, shake the flask at 28°C and 160r / min for 10 days, stop the fermentation; centrifuge the fermentation broth at 4000r / min for 10min, collect the bacteria, wash with water and dry at 105°C ...

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Abstract

The invention discloses fermentable silk spore yeast strain and a method for preparing microbial oil with lignocellulose hydrolyzate. The method for preparing the yeast strain comprises the following steps of: taking the fermentable silk spore yeast CICC 1368 as the original strains, performing the ultraviolet ray mutagenesis and the composite mutagenesis of the ultraviolet ray and the LiCl, obtaining the high-yield strains by means of fermenting and screening, and naturalizing the obtained strains in the lignocellulose hydrolyzate to finally obtain the oil yeast strain CCTCC M209060 which can effectively accumulate the oil in the lignocellulose hydrolyzate. The strains has high oil yield compared with the original strains, can effectively accumulate the oil in the lignocellulose hydrolyzate which contains the inhibitor with higher concentration, and offers the probability for preparing the microbial oil with low cost, high efficiency and large scale.

Description

technical field [0001] The invention belongs to the fields of fermentation engineering and microbial engineering, and in particular relates to a strain Trichosporium fermentum CCTCC M209060, which efficiently utilizes lignocellulose hydrolyzate to produce microbial oil, and a method for producing microbial oil by fermenting lignocellulose hydrolyzate with the strain. Background technique [0002] Biodiesel is a green renewable energy that can replace fossil diesel produced from animal and vegetable oils, and plays a very important role in the strategy of replacing petroleum energy. At present, the industrial production of biodiesel mainly uses soybean oil, rapeseed oil and waste oil as raw materials. my country has a large population and a small land, so the production of biodiesel from edible oil is not in line with the national conditions, and the amount of waste oil is limited, which cannot meet the huge demand for energy in the modern economy and society. Microbial oil,...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/16C12R1/645
Inventor 吴虹黄超宗敏华
Owner SOUTH CHINA UNIV OF TECH
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