Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for producing lactic acid through continuously fermenting batches of lignocellulose hydrolysate by coupling fermenting and membrane separation

A technology of lignocellulose and hydrolyzate, applied in the field of bioengineering, can solve the problems of long cycle and low yield, and achieve the effects of improving fermentation intensity, reducing production cost and shortening fermentation cycle.

Active Publication Date: 2014-06-04
INST OF PROCESS ENG CHINESE ACAD OF SCI
View PDF6 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of long period and low yield in the process of using mixed sugars in lignocellulose hydrolyzate to ferment and produce lactic acid, the present invention utilizes the coupling technology of fermentation and membrane separation to recover cells for recycling after batch fermentation and supplement fresh culture medium , continuous batch fermentation, to increase the utilization rate of carbon source and increase the yield of lactic acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing lactic acid through continuously fermenting batches of lignocellulose hydrolysate by coupling fermenting and membrane separation
  • Method for producing lactic acid through continuously fermenting batches of lignocellulose hydrolysate by coupling fermenting and membrane separation
  • Method for producing lactic acid through continuously fermenting batches of lignocellulose hydrolysate by coupling fermenting and membrane separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for producing lactic acid by fermentation of the mixed sugar in the hydrolysate of wheat straw in open cell recycling.

[0026] The composition of each medium used in this embodiment is as follows:

[0027] MRS medium (peptone 10g / L, beef powder 10g / L, yeast powder 0.5g / L, anhydrous sodium acetate 0.5g / L, hydrogen citrate diamine 0.2g / L, dipotassium hydrogen phosphate 0.2g / L) . The MRS medium added 10g / L glucose is the seed medium.

[0028] The specific method of this embodiment includes the following steps:

[0029] 1. Preparation of wheat straw hydrolysate: use 2% (w / v) sulfuric acid to treat wheat straw powder at 121°C for 90 minutes, with a solid-to-liquid ratio of 1:10, and the liquid obtained after filtering the pretreatment mixture is the wheat straw dilute acid Hydrolyzate. Use cellulase to enzymatically decompose the solid material obtained after dilute acid pretreatment (the main component is cellulose), and add 20FPU cellulase per gram of cellulose. En...

Embodiment 2

[0038] The method of open cell cycle recycling and utilization of mixed sugar in the bagasse hydrolysate to produce lactic acid by fermentation.

[0039] The seed culture medium formula used in this example is the same as in example 1.

[0040] The specific method of this embodiment includes the following steps:

[0041] 1. Preparation of bagasse hydrolysate: bagasse is dried and crushed into powder with an average size of 40 mesh. The bagasse powder is treated by ammonia steam explosion. The treatment conditions are: pressure 1.5MPa, maintenance for 10 min, solid-liquid ratio 1:6 (mass volume ratio), and ammonia water dosage is 5%. After pretreatment, solid-liquid separation obtains the pretreatment liquid and the bagasse solids (the main component is cellulose). Use cellulase to enzymatically hydrolyze bagasse solids, add 20FPU cellulase per gram of cellulose, enzymatic hydrolysis conditions: 50℃, pH5.0, 200rpm, enzymatic hydrolysis time 48h. After the enzymolysis, the pretreatm...

Embodiment 3

[0047] The method of open cell cycle recycling and utilization of mixed sugar in switchgrass hydrolysate to produce lactic acid by fermentation.

[0048] The seed culture medium formula used in this example is the same as in example 1.

[0049] 1. Preparation of switchgrass hydrolysate: switchgrass is dried and crushed into powder with an average size of 40 mesh. Switchgrass powder is pretreated with dilute acid under the conditions: pressure 135℃, 10min, 2% (w / v) sulfuric acid, solid-liquid ratio 1:8 (mass volume ratio). After pretreatment, after pretreatment, solid-liquid separation obtains the pretreatment liquid and switchgrass solids (the main component is cellulose). Use cellulase to enzymatically hydrolyze switchgrass solids, add 20FPU cellulase per gram of cellulose, enzymatic hydrolysis conditions: 50℃, pH5.0, 200rpm, enzymatic hydrolysis time 48h. After the enzymolysis, the pretreatment solution and enzymolysis solution were combined, and concentrated using a rolled pol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing lactic acid through continuously fermenting batches of lignocellulose hydrolysate by coupling fermenting and membrane separation. According to the method, bacillus coagulans CGMCCC (China General Microbiological Culture Collection Center) No. 7635 is taken as the fermented culture, and mixed sugar in the lignocellulose hydrolysate is taken as the carbon source. Aiming at the defects in the fermentation process that unsynchronized utilization of hexose and pentose by microorganism results in longer fermentation period and low yield, the invention provides a method for cell recycling and continuous batch fermentation through coupling of membrane separation units and fermentation, and the method comprises steps of activation of culture, seed culture, fermentation cultivation, filtration of ultrafiltration membrane, cell recycling and batch fermentation. By adopting the method, the fermentation period can be effectively shortened, and the utilization efficiency of the mixed carbon source of hexose and pentonse by the thallus is improved. The method has mild operation conditions, has good stability, has general guiding significance in production of biochemical products through fermenting by the microorganism using the mixed sugar in the lignocellulose hydrolysate, and has wide industrial application prospect.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for coupling fermentation and membrane separation to realize continuous batch fermentation of lignocellulose hydrolysate to produce lactic acid. Background technique [0002] Lactic acid is an important organic acid, which is widely used in the fields of food, medicine, chemical industry, brewing and textile. Its molecular structure contains an asymmetric carbon atom and two optical isomers, D and L. In recent years, polylactic acid plastic synthesized with lactic acid as a monomer is considered to be one of the best substitutes for petrochemical plastics due to its biodegradable properties. [0003] At present, lactic acid is mainly produced by fermentation, and the fermentation method is batch fermentation, that is, each fermentation needs to cultivate one or even multiple levels of seeds. Seed cultivation not only extends the fermentation cycle, but also i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/56C07C59/08C07C51/47C12R1/07
Inventor 万印华张玉明陈向荣齐本坤沈飞苏仪
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com