Actinobacillus succinogenes strain YH123 and application thereof

A technology of YH123 and Actinobacillus, which is applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of low succinic acid production capacity, poor sugar or succinic acid tolerance, and no use value, etc., to achieve Significant social significance and economic value, high conversion rate of sugar, high yield effect

Inactive Publication Date: 2012-10-17
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Natural strains have relatively low succinic acid-producing ability, various fermentation products, poor tolerance to sugar or succinic acid, and basically no use value

Method used

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  • Actinobacillus succinogenes strain YH123 and application thereof
  • Actinobacillus succinogenes strain YH123 and application thereof
  • Actinobacillus succinogenes strain YH123 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Actinobacillus succinate ( Actinobacillus succinogenes ) NJ113 method for the first step of plasma mutagenesis

[0028] The starting strain Actinobacillus succinogenes used in the present invention ( Actinobacillus succinogenes) Source of NJ113: The patent procedure deposit (Deposit No.: CGMCC No. 1716) has been carried out in the General Microbiology Center of the China Microbiological Culture Collection Management Committee recognized by the China Patent Office or the International Patent Organization, and has been authorized before the date of this application ( Patent No. 200610085415.9, authorized announcement date September 9, 2009, authorized announcement number CN100537744

[0029] C) Biomaterials.

[0030] Actinobacillus succinogenes ( Actinobacillus succinogenes ) NJ113 original strain activation culture, culture temperature 35 ~ 37 ℃, 100mL serum bottle volume of 40 ~ 60mL, filled with CO 2 2 to 3 minutes, culture time 8 to 10...

Embodiment 2

[0031] Embodiment 2: Actinobacillus succinate ( Actinobacillus succinogenes ) Screening method for YH123

[0032] Screening steps:

[0033] 1. Preliminary screening of bromothymol blue plate

[0034] Place the mutagenized slide in a stoppered test tube filled with 0.5-2mL of normal saline, shake it fully, elute the bacteria on the slide, and apply an appropriate amount to bromothymol blue (0.10-0.25 g / L) medium plate, anaerobic culture at 35~37℃ for 12~36h, and 45 colonies whose color change circle was obviously larger than the starting bacteria were selected.

[0035] 2. Re-screening with bromothymol blue plate

[0036] Inoculate the screened strains into a 100mL serum bottle with a liquid volume of 50mL and fill with CO 2 2min, 35~37℃, anaerobic culture for 10~12h, make a bacterial suspension with a concentration of OD=0.1 in sterile normal saline, pipette 10μL and spread it on the solid medium containing bromothymol blue, 35~37 After anaerobic cu...

Embodiment 3

[0051] Embodiment 3: Actinobacillus succinate ( Actinobacillus succinogenes ) Passage stability of YH123

[0052] In the fermentation medium with glucose as the carbon source, the passage stability of the mutant strain YH123 was detected, and the results of the passage fermentation test of the strain YH123 are shown in Table 2:

[0053]

[0054] From the experimental results, it can be seen that after 7 consecutive passages, the production and yield of succinic acid of Actinobacillus succinogenes YH123 are relatively stable, and it has good passage stability, which can be used as a production strain for further research and development.

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Abstract

The invention relates to an actinobacillus succinogenes strain YH123 with a collection number of CCTCC NO: M2012072. The invention also relates to the application of the actinobacillus succinogenes strain YH123 in the fermentation production of succinic acid. The strain can highly efficiently employ different carbon sources in fermentation productions of succinic acid, and the conversion rate of saccharide is high. When glucose is adopted as a carbon source, in a fermentation tank with a volume of 3L, the output and yield of succinic acid respectively reaches 33.2g / L and 66.7%, which are respectively improved by 18.2% and 10.3% than those of start bacteria.

Description

technical field [0001] The invention relates to an Actinobacillus succinic acid producing strain YH123 and its application, specifically its application in the succinic acid fermentation industry, belonging to the technical field of industrial microorganisms and its fermentation. Background technique [0002] Succinic acid (also known as succinic acid) is a common natural organic acid widely found in human body, animals, plants and microorganisms. As an important chemical raw material, it is also widely used in pharmaceutical, food and surfactant industries. Succinic acid can be used as an intermediate in the synthesis of complex organic compounds, and also as a C 4 Platform compounds are used in the synthesis of bulk chemicals and biodegradable materials, so succinic acid is considered to be the most marketable fermented organic acid product after citric acid. [0003] The traditional chemical synthesis of succinic acid is produced by electrolysis of butane through male...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/46C12R1/01
Inventor 姜岷徐蓉奚永兰陈可泉张九花戴文宇韦萍
Owner NANJING UNIV OF TECH
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