Actinobacillus succinogenes strain YH123 and application thereof
A technology of YH123 and Actinobacillus, which is applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of low succinic acid production capacity, poor sugar or succinic acid tolerance, and no use value, etc., to achieve Significant social significance and economic value, high conversion rate of sugar, high yield effect
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Embodiment 1
[0027] Embodiment 1: Actinobacillus succinate ( Actinobacillus succinogenes ) NJ113 method for the first step of plasma mutagenesis
[0028] The starting strain Actinobacillus succinogenes used in the present invention ( Actinobacillus succinogenes) Source of NJ113: The patent procedure deposit (Deposit No.: CGMCC No. 1716) has been carried out in the General Microbiology Center of the China Microbiological Culture Collection Management Committee recognized by the China Patent Office or the International Patent Organization, and has been authorized before the date of this application ( Patent No. 200610085415.9, authorized announcement date September 9, 2009, authorized announcement number CN100537744
[0029] C) Biomaterials.
[0030] Actinobacillus succinogenes ( Actinobacillus succinogenes ) NJ113 original strain activation culture, culture temperature 35 ~ 37 ℃, 100mL serum bottle volume of 40 ~ 60mL, filled with CO 2 2 to 3 minutes, culture time 8 to 10...
Embodiment 2
[0031] Embodiment 2: Actinobacillus succinate ( Actinobacillus succinogenes ) Screening method for YH123
[0032] Screening steps:
[0033] 1. Preliminary screening of bromothymol blue plate
[0034] Place the mutagenized slide in a stoppered test tube filled with 0.5-2mL of normal saline, shake it fully, elute the bacteria on the slide, and apply an appropriate amount to bromothymol blue (0.10-0.25 g / L) medium plate, anaerobic culture at 35~37℃ for 12~36h, and 45 colonies whose color change circle was obviously larger than the starting bacteria were selected.
[0035] 2. Re-screening with bromothymol blue plate
[0036] Inoculate the screened strains into a 100mL serum bottle with a liquid volume of 50mL and fill with CO 2 2min, 35~37℃, anaerobic culture for 10~12h, make a bacterial suspension with a concentration of OD=0.1 in sterile normal saline, pipette 10μL and spread it on the solid medium containing bromothymol blue, 35~37 After anaerobic cu...
Embodiment 3
[0051] Embodiment 3: Actinobacillus succinate ( Actinobacillus succinogenes ) Passage stability of YH123
[0052] In the fermentation medium with glucose as the carbon source, the passage stability of the mutant strain YH123 was detected, and the results of the passage fermentation test of the strain YH123 are shown in Table 2:
[0053]
[0054] From the experimental results, it can be seen that after 7 consecutive passages, the production and yield of succinic acid of Actinobacillus succinogenes YH123 are relatively stable, and it has good passage stability, which can be used as a production strain for further research and development.
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