Fermentation production method of succinic acid with whey

A technology of succinic acid and whey is applied in the field of bioengineering to achieve the effect of reducing the amount of nitrogen sources, reducing the composition of culture medium, and alleviating the shortage of petrochemical resources

Inactive Publication Date: 2010-10-13
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that whey is used as a carbon source for succinic acid fermentation, but the highest yield of succinic acid is only 27.9g / L, with a yield of 57%.

Method used

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  • Fermentation production method of succinic acid with whey
  • Fermentation production method of succinic acid with whey
  • Fermentation production method of succinic acid with whey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Seed culture: add seed medium to 80mL in a 100mL serum bottle, and feed N 2 and CO 2 mixed gas; after cooling, insert Actinobacillus succinogenes NJ113 for cultivation;

[0029] The culture conditions are: temperature 40°C, shaker speed 200r / min, culture time 16h;

[0030] (2) Fermentation acid production: add fermentation medium volume to 5L fermenter tank and be 4L, inoculum size is 7% (v / v), temperature is 40 DEG C, pass into containing N 2 and CO 2 The mixed gas is used to maintain the anaerobic environment of the fermentation system, and the stirring speed is 300rpm and the fermentation time is 60h.

[0031] Seed medium (g / L): Glucose 10 (dissolved), yeast extract 5, NaHCO 3 10, NaH 2 PO 4 2H 2 O 9.6, K 2 HPO 4 ·3H 2 O 15.5; pH 6.8.

[0032] Fermentation medium (g / L): whey (based on lactose content) 30-100, yeast extract 10, KH 2 PO 4 3. MgCl 2 ·6H 2 O0.3, CaCl 2 0.3, NaCl 1, Na 2 HPO 4 0.31, NaH 2 PO 4 1.6.

[0033] Fermentation exper...

Embodiment 2

[0039] (1) Seed culture: add seed medium to 20mL in a 100mL serum bottle, and feed N 2 and CO 2 mixed gas; after cooling, insert Actinobacillus succinogenes NJ113 for cultivation;

[0040]The culture conditions are: temperature 30°C, shaker speed 100r / min, culture time 10h;

[0041] (2) Fermentation acid production: Add fermentation medium volume to 5L fermenter tank and be 2L, inoculum size is 3% (v / v), temperature is 35 ℃, pass into containing N 2 and CO 2 The mixed gas is used to maintain the anaerobic environment of the fermentation system, and the stirring speed is 100rpm and the fermentation time is 30h.

[0042] According to the method of example 1, carry out fermentation experiment in serum bottle, with the whey after the vacuum drying as carbon source, add the yeast extract of different concentration in fermentation medium, its content is (g / L): 0g, 2g , 4g, 5g, 6g, 6.5g, 7g, 8g, 9g, 10g. The effects of different yeast extract concentrations on the production of ...

Embodiment 3

[0047] In this example, whey is used as a carbon source in the process of preparing succinic acid by the biological method, and the ability of bacterial cell fermentation to prepare succinic acid is realized by using it in combination with a small amount of yeast extract (6g / L). Bacterial classification, concrete culture method are with embodiment 1. The initial whey is 70g / L (calculated as lactose).

[0048] (1) Seed culture: add seed medium to 50mL in a 100mL serum bottle, and feed N 2 and CO 2 mixed gas; after cooling, insert Actinobacillus succinogenes NJ113 for cultivation;

[0049] The culture conditions are: temperature 37°C, shaker speed 150r / min, culture time 12h;

[0050] (2) Fermentation acid production: Add fermentation medium volume to 5L fermenter tank and be 3L, inoculum size is 5% (v / v), temperature is 37 ℃, pass into containing N 2 and CO 2 The mixed gas is used to maintain the anaerobic environment of the fermentation system, and the stirring speed is 18...

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Abstract

The invention relates to a fermentation production method of succinic acid with whey, which adopts the technical scheme that: actinobacillus succinogenes NJ113 is put in a culture medium of whey containing 30-100g / L of lactose to produce succinic acid by fermentation. The invention utilizes actinobacillus succinogenes selected from rumen which is suitable for fermenting whey raw material to produce the succinic acid, so that the consumption of a nitrogen source can be effectively reduced, the product concentration is up to 47.51g / L, and the yield is up to 67.9%; and the actinobacillus succinogenes is very suitable for industrial production of the succinic acid in low cost. The invention has outstanding advantages that: cheap whey raw material is utilized to produce the succinic acid by fermentation, and the whey as an cheap crude raw material can substitute glucose to be used as a carbon source to produce the succinic acid by fermentation; the method is a production method utilizing renewable raw materials, can alleviate the shortages of petroleum resources for chemical synthesis of the succinic acid, and is environmentally friendly; and the whey contains the nitrogen source and other nutrients which can be utilized by microorganisms, thereby obviously reducing the fermented culture medium components.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and mainly relates to a method for producing succinic acid by fermenting cheap whey raw materials. Background technique [0002] Succinic acid, also known as succinic acid, is a common natural organic acid that is widely found in the human body, animals, plants, and microorganisms. Succinic acid is an important chemical that can be used in surfactants, detergents, solvents, etc. It can be used as a pH modifier, flavoring agent or antibacterial agent in the food industry, and can also be used as an animal feed additive or plant growth agent Wait. In industry, succinic acid is an important C4 platform compound, which can be converted into many important chemicals, such as fatty acids, tetrahydrofuran, 1,4-butanediol, γ-butyrolactone, etc. The body is used to synthesize the biodegradable material PBS. At present, more than 250 kinds of chemical products using benzene as raw materials can b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12R1/01
Inventor 姜岷杨卓娜李建方晓江郑晓宇奚永兰马江峰韦萍
Owner NANJING UNIV OF TECH
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