Genes from actinobacillus succinogenes 13oz (atcc 55618) for production of chemicals from the a. succinogenes C4-pathway

Abstract

Actinobacillus succinogenes genes and methods of using the genes in genetically engineered A. succinogenes so as to improve production of chemicals such as succinate, fumarate, malate, 5-aminolevulinate, 2-oxoglutarate, glutamate, and aspartate. The genetically engineered A. succinogenes strains are capable of overexpressing C4 enzymes. The genetically engineered A. succinogenes can have one or more gene knockouts or modifications that inhibit C3 enzymes. The fluxes supplying substrate to the C4 pathway can also be improved in some of the genetically engineered A. succinogenes.

Description

[0001] Cross References to Related Applications [0001] This application claims the benefit of Provisional Application No. 60 / 705,841, filed August 5, 2005, which is hereby incorporated by reference in its entirety. Statement Regarding Federally Funded Research or Development [0002] This work was supported by funding from the National Science Foundation. It was made possible by the genome-sequencing efforts of the Department of Energy's Joint Genome Institute. The US Government has certain rights in this invention. Background of the invention a.Technical field [0003] The present invention relates generally to metabolic engineering, and more particularly to engineering Actinobacillus succinogenes to produce Chemicals such as succinate, fumarate, malate, 5-aminolevulinic acid ( 5-aminolevulinate), 2-oxoglutarate, glutamate, and aspartate. In particular, the invention relates to inhibiting C3 pathway enzymes and overexpressing C4 pathway enzymes. The present inven...

AI Technical Summary

Problems solved by technology

Much effort has been devoted to engineering E. coli strains to produce large amounts of succinate, but none of the engineered E. coli strains surpassed A. succinogenes in succinate production

Images