35 results about "2-Oxoglutarate" patented technology

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and / or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.

A D-aminotransferase can be modified so as to efficiently produce (2R, 4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R, 4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.

A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities of a polypeptide selected from the group consisting of 2-oxoglutarate-dependent dioxygenase, 3-ketoacyl-CoA thiolase, 3′-phosphoadenosine 5′-phosphate phosphatase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, 5OS chloroplast ribosomal protein L21, 57972199. R01.1-protein, 60952769. R01.1-protein, 60S ribosomal protein, ABC transporter family protein, AP2 domain-containing transcription factor, argonaute protein, AT1 G29250.1-protein, AT1 G53885-protein, AT2G35300-protein, AT3G04620-protein, AT4G01870-protein, AT5G42380-protein, AT5G47440-protein, CDS5394-protein, CDS5401_TRUNCATED-protein, cold response protein, cullin, Cytochrome P450, delta-8 sphingolipid desaturase, galactinol synthase, glutathione-S-transferase, GTPase, haspin-related protein, heat shock protein, heat shock transcription factor, histone H2B, jasmonate-zim-domain protein, mitochondrial asparaginyl-tRNA synthetase, Oligosaccharyltransferase, OS02G44730-protein, Oxygen-evolving enhancer protein, peptidyl-prolyl cis-trans isomerase, peptidyl-prolyl cis-trans isomerase family protein, plastid lipid-associated protein, Polypyrimidine tract binding protein, PRLI-interacting factor, protein kinase, protein kinase family protein, rubisco subunit binding-protein beta subunit, serine acetyltransferase, serine hydroxymethyltransferase, small heat shock protein, S-ribosylhomocysteinase, sugar transporter, Thioredoxin H-type, ubiquitin-conjugating enzyme, ubiquitin-protein ligase, universal stress protein family protein, and Vacuolar protein.

A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.

The present invention addresses the issue of providing a method whereby hydroxy-L-lysine can be efficiently produced. The present invention provides a production method for hydroxy-L-lysine, characterized by: causing a 2-oxoglutarate-dependent L-lysine hydroxylase, cells including same, a preparation of said cells, or a culture fluid obtained by cultivating said cells, to act on L-lysine; and generating the hydroxy-L-lysine indicated in general formula (I) (in the formula, R1, R2, and R3 each indicate a hydrogen atom or a hydroxyl group and at least one among R1, R2, and R3 indicates a hydroxyl group.)

Methods and kits for analysing a sample from a test subject. The methods involve determining the level of at least one compound selected from the group consisting of N-acetylglutamate, methionine, acetylcarnitine, indole-3-acetate, 2-oxoglutarate, anserine, aspartate and butyrate in the sample from the test subject; and comparing the level of the at least one compound determined to at least one control level, wherein the levels of the at least one compound are indicative of whether the subject has hepatic cancer.

The invention belongs to the technical field of genetic engineering and fermentation, and particularly discloses an engineering bacterium for producing 3-hydroxybutyric acid and 4-hydroxybutyric acid copolyester (P3HB4HB) by utilizing a sugar carbon source. Exogenous genes needed for combining the P2HB4HB are recombined and integrated on the genome of the engineering bacterium and comprise poly-3-hydroxybutyrate synthetic gene phaCAB and 4-hydroxybutyryl coenzyme A which is transferase gene orfZ, 4-hydroxybutyric acid dehydrogenase gene 4hbD and 2-oxoglutarate dehydrogenase gene kgd. By utilization of the engineering bacterium, the P3HB4HB can be produced by using the sugar carbon source with relatively cheap price, the production cost is effectively reduced, and the large-scale industrial production and the commercial application and development are pushed.

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize 2-oxoglutarate-dependent oxygenase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Provided herein are methods for detecting and quantifying succinate in a sample. Also provided are methods for detecting and quantifying 2-oxoglutarate oxygenase enzyme and / or 2-oxoglutarate oxygenase activity in a sample and methods for screening for modulators of 2-oxoglutarate oxygenase activity.

The invention relates to fire retardant for a car seat and belongs to the field of flame retardant products. The fire retardant for the car seat is prepared from the following raw materials: water, tetraethylene glycol dimethacrylate, endo-1,7,7-trimethylbicyclo[2.2.1]hept-2-alcohol acetate, all-cis-6,9,12-octadecartrienoic acid, disodium 2-oxoglutarate dehydrate, 2-amino-2-hydroxymethyl-1,3-propylene glycol, 2-propargyl-3-methyl-4-hydroxyl-2-cyclopentene-1-ketone and trans-4-Hydroxycinnamic acid. The fire retardant for the car seat is formed by interreaction on material components, is stable in chemical properties, hardly decomposes at high temperature, attaches on the fabric or leather surface without influencing the properties of the fire retardant product, also can effectively prevent a flame from spreading and avoid a fire of the car seat, has excellent flame retardant efficiency and is applicable to popularization and application in the field of car seats.

The present invention aims to provide a method for efficiently producing hydroxy-L-lysine. The present invention provides a method for producing hydroxy-L-lysine, the method comprising allowing 2-oxoglutarate-dependent L-lysine hydroxylase, a cell containing 2-oxoglutarate-dependent L-lysine hydroxylase, a processed product of the cell, and / or a culture broth obtained by culturing the cell, to act on L-lysine to produce hydroxy-L-lysine represented by the following General Formula (I) (wherein each of R1, R2 and R3 represents a hydrogen atom or hydroxyl group, with the proviso that at least one of R1, R2 and R3 represents a hydroxyl group).

The invention discloses diaminobutyrate-2-oxoglutarate transaminase and application thereof. The diaminobutyrate-2-oxoglutarate transaminase has an amino acid sequence shown in SEQ ID NO:2. The invention also discloses an encoding gene of the diaminobutyrate-2-oxoglutarate transaminase, wherein the encoding gene has a nucleotide sequence shown in SEQ ID NO:1. The invention also discloses an expression vector containing the encoding gene of the diaminobutyrate-2-oxoglutarate transaminase, a genetic engineering strain and a construction method thereof. In the invention, an expressed diaminobutyrate-2-oxoglutarate transaminase gene is extracted, sequenced and cloned from Streptomyces albulus for the first time; and the genetic engineering strain is constructed and L-2,4-diaminobutyrate is fermented and produced by utilizing the genetic engineering strain, so that a novel L-2,4-diaminobutyrate acquisition way is opened up.