Hydrogen-containing organ transplantation protective liquid and preparation method thereof

A technology of protective liquid and hydrogen, which is applied in the field of medical devices, can solve the problems of large dosage, side effects of organ protection, etc., and achieve low cost, enhanced preservation effect, and enhanced effect

Inactive Publication Date: 2019-01-29
韩城维康水科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of mannitol in the existing traditional organ protection solution is relatively large, which has certain side effects on organ protection

Method used

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  • Hydrogen-containing organ transplantation protective liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing a hydrogen-containing organ transplant protection solution, comprising the steps of:

[0026] (1) Preparation of raw materials: water for injection, NaCl, KCl, MgCl that meet the national drug requirements 2 ·6H 2 O, Histidine·HCl·H 2 O, Histidine, Tryptophan, Mannitol, CaCl 2 2H 2 O. Potassium hydrogen 2-ketoglutarate was weighed in proportion, and set aside at 4°C;

[0027] (2), product formula: every 1000 milliliter solution contains NaCl 15mmol, KCl 9mmol, MgCl 2 ·6H 2 O 4mmol, histidine·HCl·H 2 O 18mmol, histidine 180mmol, tryptophan 2mmol, mannitol 15mmol, CaCl 2 2H 2 O0.015mmol, potassium hydrogen 2-ketoglutarate 1mmol, and the rest are water for injection;

[0028] (3) Add water for injection into the mixing tank first, then add NaCl, KCl, histidine·HCl·H in sequence 2 O, histidine, MgCl 2 ·6H 2 O, Mannitol, Potassium hydrogen 2-ketoglutarate, Tryptophan, CaCl 2 2H 2 0 is mixed, stirring speed 20 revs / min; Need to stir 2min a...

Embodiment 2

[0035] Live organ harvesting was performed on 10 boars, including heart, lung, kidney, and liver. Among them, the living organs of 5 boars used the existing protection solution HTK liquid (its components include: sodium chloride 15mmol, potassium chloride 9mmol, Magnesium chloride hexahydrate 4mmol, histidine monohydrochloride 18mmol, histidine 180mmol, tryptophan 2mmol, calcium chloride dihydrate 0.015mmol, mannitol 30mmol, 2-ketoglutarate potassium hydrogen 1mmol and water for injection 1000ml. ) for 6 hours, and the other 5 living organs were treated with the protection solution prepared in Example 1 of the present invention for 6 hours, and the oxidation-reduction potential (ORP) results obtained after measurement are shown in Table 1.

[0036] Table 1 Comparison of ORP changes in organ protection by protective solutions

[0037]

[0038] According to the test comparison table Table 1, it shows that the protective solution prepared by the embodiment of the present inventi...

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Abstract

The invention relates to a hydrogen-containing organ transplantation protective liquid and a preparation method thereof. Each liter of a water solution of the protective liquid contains 14-16 mmol ofNaCl, 8-10 mmol of KCl, 3-5 mmol of MgCl2.6H2O, 16-20 mmol of histidine.HCl.H2O, 170-190 mmol of histidine, 1-3 mmol of tryptophan, 10-20 mmol of mannitol, 0.015 mmol of CaCl2.2H2O, 0.8-1.2 mmol of potassium hydrogen 2-oxoglutarate and 9-11 mmol of hydrogen. The hydrogen-containing organ transplantation protective liquid provided by the invention effectively enhances the in-vitro organ preservation effect. According to the invention, the hydrogen is adopted, and the hydrogen and the mannitol act on oxygen free radicals at a same time, so that the effect of anti-oxidation free radicals is enhanced, and side effects of the mannitol are reduced.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a hydrogen-containing organ transplant protection solution and a preparation method thereof. Background technique [0002] Organ transplantation is a surgery in which a healthy organ is transplanted into another human body to restore its function quickly, with the purpose of compensating for the loss of function of the recipient's corresponding organ due to a fatal disease. Organ transplantation in a broad sense includes cell transplantation and tissue transplantation. If the donor who donates the organ and the recipient who receives the organ are the same person, this kind of transplant is called autologous transplantation; although the donor and the recipient are not the same person, but the donor and the recipient have exactly the same genetic quality, this kind of transplant is called autologous transplantation. homogeneous transplantation. Human-to-human transplant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 高民权贾亚峰
Owner 韩城维康水科技有限公司
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