Method for extracting alpha-ketoglutaric acid from fermentation liquor

A technology for ketoglutaric acid and fermentation broth, which is applied in chemical instruments and methods, separation/purification of carboxylic acid compounds, preparation of carboxylate salts, etc., can solve problems such as pyruvic acid cannot be effectively separated, high discharge of waste liquid, etc. Achieve the effect of improving quality, achieving zero emissions, and improving extraction yield

Inactive Publication Date: 2015-12-30
SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for extracting α-ketoglutaric acid from fermentation broth, which solves the problem that the by-product pyruvic acid in the process of extracting α-ketoglutarate in the prior art cannot be effectively separated and the waste liquid high emissions problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Heat the fermentation broth containing α-ketoglutaric acid to 60°C, adjust the pH value to 2 with industrial sulfuric acid, and sterilize through a zirconia membrane with a pore size of 200nm. Microfiltration conditions: temperature 60°C, pressure 0.35MPa, fermentation broth Flow rate 60L / m 2 h; then go through roll membrane or hollow fiber membrane to remove impurities and decolorize, the molecular weight cut off is 6000-10000D, ultrafiltration conditions: temperature 40 ℃, pressure 0.35Mpa, fermentation broth flow rate 50L / m 2 h; the thick slurry of bacteria obtained by micro-membrane filtration is then filtered through a plate and frame, and the clear liquid obtained is added to the next batch of fermentation broth to extract α-ketoglutaric acid. The bacterial protein is dried with a spray drier at a temperature of 100 ℃, drying time is 5s.

[0044] Add Ca(OH) to the filtrate 2 Make α-ketoglutaric acid form calcium salt precipitation, keep stirring until the pH of ...

Embodiment 2

[0052] Heat the fermentation broth containing α-ketoglutaric acid to 70°C, adjust the pH value to 4 with industrial sulfuric acid or industrial hydrochloric acid, sterilize through an alumina membrane with a pore size of 200nm, and microfiltration conditions: temperature 70°C, pressure 0.35MPa , fermentation broth flow rate 150L / m 2 h; then go through roll membrane or hollow fiber membrane to remove impurities and decolorize, the molecular weight cut off is 6000-10000D, ultrafiltration conditions: temperature 60 ℃, pressure 0.2MPa, fermentation broth flow rate 100L / m 2 h; the thick slurry of bacteria obtained by micro-membrane filtration is then filtered through a plate and frame, and the clear liquid obtained is added to the next batch of fermentation broth to extract α-ketoglutaric acid. The bacterial protein is dried with a spray drier at a temperature of 150 ℃, drying time is 1s.

[0053] Add Ca(OH) to the filtrate 2 Make α-ketoglutaric acid form calcium salt precipitati...

Embodiment 3

[0061] Heat the fermentation broth containing α-ketoglutaric acid to 65°C, adjust the pH value to 3 with industrial sulfuric acid or industrial hydrochloric acid, sterilize through a ceramic membrane with a pore size of 200nm, microfiltration conditions: temperature 65°C, pressure 0.25MPa, Fermentation broth flow rate 100L / m 2 h; then go through roll membrane or hollow fiber membrane to remove impurities and decolorize, the molecular weight cut-off is 6000-10000D, ultrafiltration conditions: temperature 50 ℃, pressure 0.25Mpa, fermentation broth flow rate 80L / m 2 h; the thick slurry of bacteria obtained by micro-membrane filtration is then filtered through plate and frame, and the clear liquid is added to the next batch of fermentation broth to extract α-ketoglutaric acid. The bacteria protein is dried with a spray drier at a temperature of 120 ℃, drying time is 3s.

[0062] Add Ca(OH) to the filtrate 2 Make α-ketoglutaric acid form calcium salt precipitation, keep stirring ...

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Abstract

The invention discloses a method for extracting alpha-ketoglutaric acid from fermentation liquor. The method comprises the following steps: heating fermentation liquor containing the alpha-ketoglutaric acid for sterilization and decolorization, and drying mycoprotein; adding Ca(OH)2 into a filtration liquid, and performing centrifugal separation to obtain calcium 2-oxoglutarate and Ca(OH)2; adding H2SO4 into the calcium 2-oxoglutarate and Ca(OH)2, and performing centrifugal separation to obtain CaSO4 sediments; enabling the filtration liquid to flow into an ion exchange column for adsorption, and adding activated carbon into eluant for decolorization for 30 min; performing pressure reduction evaporation on the high-concentration alpha-ketoglutaric acid solution to obtain alpha-ketoglutaric acid crystals, and performing centrifugal separation to obtain an alpha-ketoglutaric acid crude product, washing the alpha-ketoglutaric acid crude product with methanol, removing impurities through washing, and performing centrifugation to remove methanol, so as to obtain the alpha-ketoglutaric acid, wherein the volume ratio of the alpha-ketoglutaric acid crude product to the methanol is 5:1. Through the adoption of the method, the problems in the prior art that pyruvic acid as a by-product during the extraction process of the alpha-ketoglutaric acid cannot be effectively separated and the discharge amount of waste liquid is high are solved.

Description

technical field [0001] The invention belongs to the technical field of fermentation broth extraction, and relates to a method for extracting α-ketoglutarate from fermentation broth. Background technique [0002] The traditional extraction process is mainly to extract α-ketoglutarate through membrane filtration and resin column, but this method cannot be used when the fermentation broth contains analogues of α-ketoglutarate (such as pyruvic acid) okay. Because membrane filtration and resin column cannot effectively separate the pyruvate by-product. The present invention introduces Ca(OH) in the extraction process 2 , is the use of α-ketoglutarate calcium and pyruvate calcium pyruvate different water solubility characteristics to separate the two substances. In addition, the discharge of waste liquid in the traditional extraction process is relatively large, and the present invention mainly aims at the repeated recycling of waste liquid to reduce the discharge of waste liqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C59/347C07C51/42C07C51/487C07C51/47
CPCC07C51/42C07C51/47C07C51/487C07C59/347
Inventor 王东阳蔡传康刘朋朋陈正戈连峰
Owner SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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