Method for producing succinic acid by multistage continuous fermentation

A succinic acid and fermenter technology, which is applied in the field of succinic acid preparation, can solve problems such as high cost, serious pollution, and unsustainable development, and achieve the effects of easy operation, simple method, and high output

Inactive Publication Date: 2015-02-25
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chemical method mainly uses non-renewable petroleum resources as raw materials, which has high cost, serious pollution and unsustainable development, which seriously limits the development of succinic acid

Method used

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  • Method for producing succinic acid by multistage continuous fermentation
  • Method for producing succinic acid by multistage continuous fermentation
  • Method for producing succinic acid by multistage continuous fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for producing succinic acid by a multistage continuous fermentation device, comprising the following steps:

[0029] The first step is to match the seed medium, the initial fermentation medium, the carbon source feed tank culture solution and the medium feed tank culture solution. The formula is as follows:

[0030] Seed medium (g L -1 ): glucose 10 (dissolved), yeast extract 5, corn steep liquor 5, NaHCO 3 10. NaH 2 PO 4 ·2H 2 O 9.6, K 2 HPO 4 ·3H 2 O 15.5, pH 7.0.

[0031] Initial fermentation medium (g L -1 ): glucose 40 (dissolved), yeast extract 10, corn steep liquor 10, sodium acetate 1.36, KH 2 PO 4 3. MgCl 2 ·6H 2 O 0.2, CaCl 2 0.2, NaCl 1, Na 2 HPO 4 ·12H 2 O 0.31, NaH 2 PO 4 ·2H 2 O 1.6, pH 7.0.

[0032] Carbon source feeding tank culture solution (g L -1 ): Glucose 750.

[0033] Medium feed tank culture solution (g L -1 ): yeast extract 30, corn steep liquor 30, sodium acetate 4.08, KH 2 PO 4 9. MgCl 2 ·6H 2 O 0.6,...

Embodiment 2

[0038] The first to third steps are the same as in Example 1, wherein the carbon source feeding tank culture solution is a 200 g / L sucrose solution.

[0039] The fourth step, after connecting five sterilized fermenters in series, insert 600ml of initial fermentation medium into the primary fermenter, and feed CO with a volume ratio of 1:1. 2 and H 2 Mixed gas, use the air storage tank to control the ventilation volume to 0.01L / (min·L), and insert the fermentation strain according to the inoculation volume of 10% (v / v), and the initial fermentation medium flows from the primary fermenter to the secondary, From the third level to the fifth level, the carbon source feeding tank and the medium feeding tank are used to control the inflow rate of the fermentation broth to 120-150 ml / h, the sucrose supplementation rate to be 30 ml / h, and the outflow rate of the fermentation broth to be 150-160 ml / h h, so that the initial sugar concentration in the primary fermenter is 150g / L, and in...

Embodiment 3

[0041] The first to third steps are the same as those in Example 2.

[0042] The fourth step is to connect the five sterilized fermenters in series, insert 600ml of initial fermentation medium into the primary fermenter, use the gas storage tank to control pure N2, and the ventilation rate is 0.04 L / (min·L) , according to the inoculum amount of 10% (v / v), the fermentation strain is inserted, and the initial fermentation medium flows from the first-level fermenter to the second-level, third-level to the fifth-level, and is controlled by the carbon source feeding tank and the medium feeding tank. The inflow rate of the fermentation broth is 90-110 ml / h, the sugar supplementation rate of sucrose is 20 ml / h, and the outflow rate of the fermentation broth is 110-140 ml / h, so that the initial sugar concentration in the primary fermentor is 80g / L. In the continuous fermentation process The sugar concentration in the fermenters of the middle and different levels relative to the initia...

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Abstract

The invention relates to a method for producing succinic acid by multistage continuous fermentation, which comprises the following steps: 1. preparing a seed culture medium, an initial fermentation culture medium, a carbon source supplementation tank culture solution and a culture medium supplementation tank culture solution; 2. sterilizing the cascade fermentation tank set, carbon source supplementation tank, culture medium supplementation tank, seed culture medium, initial fermentation culture medium, carbon source supplementation tank culture solution, culture medium supplementation tank culture solution and serum bottle; 3. taking the sterilized serum bottle, inoculating the sterilized seed culture medium, introducing CO2, inoculating the Actinobacillus succinogenes NJ113-producing oscillating table, and culturing to obtain a fermentation strain; and 4. carrying out multistage continuous fermentation. The production method is simple, has the advantage of high yield and purity of succinic acid, and thus, is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of succinic acid preparation, in particular to a method for producing succinic acid by multistage continuous fermentation. Background technique [0002] Succinic acid, also known as succinate acid, is a common natural organic acid and one of the intermediate metabolites of the tricarboxylic acid cycle, which is widely found in animals, plants and microorganisms. As an important C4 platform compound, succinic acid is an important intermediate product and 1,4-butanediol (BDO), γ-butyrolactone, tetrahydrofuran, N-methylpyrrolidone (NMP), adipic acid , malic acid and the new biodegradable plastic product polybutylene succinate (PBS) and other specialty chemical precursors, the total market for these chemicals is 15 billion US dollars. The latest research results also show that succinic acid has very broad prospects in the preparation and application of biodegradable plastics. The polymerization reaction of succinic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12R1/01
CPCC12P7/46
Inventor 陈可泉王震姜岷何珣肖文欧阳平凯
Owner NANJING TECH UNIV
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