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Complex microbial agent for efficiently converting heavy metal chromium in soil as well as preparation method and application thereof

The technology of compound bacterial agent and heavy metal, applied in the field of soil remediation, can solve the problems of low adaptability of heavy metal chromium, toxic growth and metabolism, long processing time, etc., and achieve the effects of short cultivation period, simple preparation and application methods, and short processing period.

Pending Publication Date: 2021-08-17
CENT SOUTH UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current relevant patents, most of the selected strains are too single. Because heavy metals have a toxic effect on the growth and metabolism of microorganisms, single strains have low adaptability to heavy metal chromium, and the treatment time is long and the effect is poor.

Method used

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  • Complex microbial agent for efficiently converting heavy metal chromium in soil as well as preparation method and application thereof
  • Complex microbial agent for efficiently converting heavy metal chromium in soil as well as preparation method and application thereof
  • Complex microbial agent for efficiently converting heavy metal chromium in soil as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A compound bacterial agent for efficiently transforming heavy metal chromium in soil, including the following two bacterial groups: 40% Bacillus, 15% Pediococcus lactis, 20% Clostridium korsi, 15% Actinobacillus succinogenes and 10% Acetobacter constitutes group A; Acidophilus ferrooxidans, ferrothiobacillus, acidothiobacillus, sulfur oxidizing bacteria and acidophilus acidothiobacillus in a quantitative ratio of 1:1:2:1.5:1.5 Bacteria group B is formed; the bacillus includes bacillus stearothermophilus, bacillus cereus and bacillus pumilus, and the quantity ratio of the three kinds of bacteria is 3:2:1.

[0033] The preparation method of above-mentioned composite microbial agent comprises the following steps:

[0034] (1) Place each bacterial strain in 100ml sterilized culture medium containing chromium ions respectively, and domesticate 25 generations at a rotating speed of 150rpm and a temperature of 28°C. The concentration gradient of chromium ions during the first ...

Embodiment 2

[0038] A compound bacterial agent for efficiently transforming heavy metal chromium in soil, including the following two bacterial groups: 45% Bacillus, 15% Pediococcus lactis, 15% Clostridium corinii, 20% Actinobacillus succinate and 5% Acetobacter constitutes group A; acidophilus ferrooxidans, ferrothiobacillus, acidothiobacillus, sulfur-oxidizing bacteria and acidophilus acidothiobacillus in a quantitative ratio of 1:2:3:2:1.5 Consisting of flora B; the bacillus includes bacillus stearothermophilus, bacillus cereus and bacillus pumilus, and the ratio of the three bacteria is 3:3:1.

[0039] The preparation method of above-mentioned composite microbial agent comprises the following steps:

[0040] (1) Place each bacterial strain in 100ml sterilized culture medium containing chromium ions respectively, and domesticate 25 generations at a rotating speed of 180rpm and a temperature of 26°C. The concentration gradient of chromium ions during the first generation to the 20th gene...

Embodiment 3

[0044] A compound bacterial agent for efficiently transforming heavy metal chromium in soil, including the following two bacterial groups: 50% Bacillus, 10% Pediococcus lactis, 5% Clostridium korshii, 25% Actinobacillus succinate and 10% Acetobacter constitutes group A; acidophilus ferrooxidans, ferrothiobacillus, acidothiobacillus, sulfur-oxidizing bacteria and acidophilus acidothiobacillus in a quantitative ratio of 1:1:4:2.5:2 Consisting of flora B; the bacillus includes Bacillus stearothermophilus, Bacillus cereus and Bacillus pumilus, and the ratio of the three bacteria is 3:4:1.

[0045] The preparation method of above-mentioned composite microbial agent comprises the following steps:

[0046] (1) Place each bacterial strain in 100ml sterilized culture medium containing chromium ions respectively, and domesticate for 25 generations at a rotating speed of 200rpm and a temperature of 24°C. The concentration gradient of chromium ions during the first generation to the 20th ...

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Abstract

The invention belongs to the technical field of soil remediation, and particularly relates to a complex microbial agent for efficiently converting heavy metal chromium in soil as well as a preparation method and application thereof. The complex microbial agent is obtained by mixing and carrying out enrichment culture on the following two florae: a flora A consisting of bacillus, pediococcus acidilactici, clostridium coriolis, actinobacillus succinogenes and bacillus aceticus; a flora B consisting of acidophilic iron bacteria, thiobacillus ferrooxidans, acid-thiobacillus thiooxidans, sulfur-oxidizing acid-thiobacillus thiooxidans, sulfur-oxidizing bacteria and acidophilic thiobacillus ferrooxidans. After the complex microbial agent is used for treating the chromium-contaminated soil, the chromium removal rate can reach 91% or above. The complex microbial agent is formed by culturing and mixing a plurality of strains, and the strains have a good synergistic effect and do not generate antagonism; after compounding, the adaptability to heavy metals can be improved, so that the survival and removal effects of the soil in heavy metal contaminated soil are improved. The microbial agent is simple in preparation and application method, short in culture period and treatment period and low in cost, prevents secondary pollution, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and in particular relates to a compound microbial agent for efficiently transforming soil heavy metal chromium, a preparation method and application thereof. Background technique [0002] Chromium is widely used in electroplating, printing and dyeing, tanning, metallurgy and other industries, and the pollution caused by improper treatment of chromium-containing wastewater and chromium slag is becoming more and more serious. Chromium-containing sewage irrigation is an important source of chromium in the soil. Both urban solid waste (sludge, garbage, fly ash) and phosphate fertilizers (such as serpentine or chromium slag-made calcium magnesium phosphate fertilizer) contain chromium. The agricultural use of valuable solid waste further caused the increase of chromium in farmland soil. Soil chromium pollution not only affects plant growth, but also affects human health. Chromium in plants ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36B09C1/10C12R1/07C12R1/085C12R1/145C12R1/01
CPCB09C1/10C12N1/20C12N1/36
Inventor 尹华群孟德龙李骞周志成刘勇军黎娟
Owner CENT SOUTH UNIV
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