Method for producing butane diacid by fermenting sugar grass raw material

A technology of sweet sorghum and succinic acid, applied to sweet sorghum raw materials in the preparation of succinic acid, the application field of a method, which can solve the problems that there are no research reports on the production of succinic acid by fermentation

Inactive Publication Date: 2009-12-16
YIFAN XINFU PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country's national pilot demonstration project of using non-grain raw materials such as sweet sorghum to build an annual output of 1 million tons of fuel ethanol has been successfully piloted ( http: / / china.npicp.com / touzi / detail / 1-8394.html , 2008), there is no research report on the production of succinic acid by fermentation of sweet sorghum raw materials

Method used

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  • Method for producing butane diacid by fermenting sugar grass raw material
  • Method for producing butane diacid by fermenting sugar grass raw material
  • Method for producing butane diacid by fermenting sugar grass raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Effects of different initial reducing sugar concentrations on succinic acid fermentation of sweet sorghum juice syrup:

[0050] Preparation of sweet sorghum juice syrup: sweet sorghum stalks stored at room temperature for about 3 months after harvest (purchased from Feidong Yajin Weiye Plantation, Anhui Province, containing 48.0% total reducing sugar by dry weight), washed, cut segment, squeeze the juice, and filter to obtain sweet sorghum syrup, which contains 14.3% of total reducing sugar and a juice extraction rate of 69.3%.

[0051] Seed medium (per L): glucose 5g, yeast extract 5g, K 2 HPO 4 ·3H 2 O 1.0g, NaH2 PO 4 2H 2 O 1.0g, pH 7.0. Anaerobic culture at 37°C for 16 hours.

[0052] Fermentation medium (per L): 20-80g sweet sorghum juice syrup (total reducing sugar), 15g yeast extract, 20g corn steep liquor, pH 6.5. Anaerobic culture at 37°C for 48 hours.

[0053] Use the prepared sweet sorghum juice syrup to ferment in batches with anaerobic bottles. When t...

Embodiment 2-11

[0058] Succinic acid fermentation of sweet sorghum syrup mixed with other biomass raw materials such as grains and sweet sorghum grain hydrolyzed syrup.

[0059] Preparation of sugar residue fiber hydrolysis syrup from sweet sorghum stalk after juicing: crush the sugar residue fiber of sweet sorghum stalk after juicing, treat with 1%% NaOH dilute lye, soak at 90°C for 1 hour, hydrolyze lignin, Filter to remove the black liquor residue, filter out fiber and semi-fiber; then add 50 FPU of cellulase enzyme solution for 48 hours according to the total sugar content of sugar residue fiber per g, and then use 0.5mol / L NaOH solution to adjust the pH to 6.5, directly Used for fermented ingredients.

[0060] Sweet sorghum grains, corn, wheat, sweet potato, cassava, etc. are used to prepare glucose syrup by the same crushing, liquefaction, and saccharification methods, and then used as fermentation medium ingredients.

[0061] According to the method of Example 1, use the sweet sorghum...

Embodiment 12

[0066] Production of succinic acid by batch fermentation of sweet sorghum juice syrup (5L mixing tank)

[0067] According to the method of Example 1, using the sweet sorghum juice extract prepared in Example 1, the initial reducing sugar concentration is 60.5g / L, in a 5L stirred fermenter (BIOFLO 110, New Brunswick Scientific, Edison, NJ, USA, using two groups of Batch fermentation is carried out in disc six straight blade turbine stirring paddles). The inoculum size is 5%, the fermentation temperature is 37°C, the stirring speed is 200 rpm, and the aeration is 100% CO 2 . The experimental results are shown in Table 3:

[0068] Table 3 Results of Batch Fermentation of Sweet Sorghum Juicing Syrup to Produce Succinic Acid (5L Stirred Tank)

[0069]

[0070] Batch fermentation in 5L stirred tank, 48 hours of total input into acid hydrolyzed sweet sorghum syrup is 60.5g / L in terms of final volume reducing sugar concentration, the remaining reducing sugar concentration of fer...

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Abstract

The invention provides a method for producing butane diacid by fermenting sugar grass raw material. The invention also provides an application of sugar grass raw material on preparing the butane diacid. Meanwhile, the invention also provides an application of actinobacillus succinogenes on preparing the butane diacid by using the sugar grass as raw material. The invention uses the actinobacillus succinogenes CGMCC1593 to ferment sugar grass juicing liquid glucose, or scum fiber hydrolytic syrup and sugar grass kernel hydrolytic syrup obtained by extracting juice from sugar grass straws which is hydrolyzed by enzyme or acid, or mixed syrup of the actinobacillus succinogenes and other carbonhydrate raw materials such as corn and the like, wherein the concentration of prepared butane diacid reaches 30-68g / L, the yield of the sugar is 80-90 percent (utilization rate of the sugar is 90-95 percent), and the production strength reaches 0.9-1.2g / (L / h). The method is environmental-friendly by using cheap and reproducible raw materials, and can release the petrifaction resources shortage problem of the chemical synthesized butane diacid.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. Specifically, the present invention relates to a method for producing succinic acid by fermenting cheap sweet sorghum raw materials. The invention also relates to the application of a sweet sorghum raw material in the preparation of succinic acid. The invention also relates to the application of Actinobacillus succinogenes in a method for preparing succinic acid from sweet sorghum. Background technique [0002] Succinic acid, also known as succinic acid, is an important four-carbon compound in industry. As a raw material for organic synthesis, it can be converted into many important industrial chemicals, including N-methylpyrrolidone, 1,4 -Butanediol, tetrahydrofuran, γ-butyrolactone, adipic acid, PBT resin, PBS degradable plastic, etc. These chemical products are currently synthesized from petroleum or coal chemical products (such as benzene, butane, etc.), and the resources are scarce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12N1/20C12R1/01
Inventor 过鑫富孙志浩
Owner YIFAN XINFU PHARMA CO LTD
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