Method for producing butane diacid by fermenting Jerusalem artichoke raw material

A technology of succinic acid and Jerusalem artichoke, which is applied in the field of bioengineering, can solve the problems of not seeing succinic acid, etc.

Inactive Publication Date: 2009-09-16
HANGZHOU XINFU TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] So far, there has been no research report on the production of succ

Method used

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  • Method for producing butane diacid by fermenting Jerusalem artichoke raw material
  • Method for producing butane diacid by fermenting Jerusalem artichoke raw material
  • Method for producing butane diacid by fermenting Jerusalem artichoke raw material

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0066] Example 1

[0067] Fermentation preparation of black koji inulinase

[0068] Aspergillus niger (Apergillus niger) CGMCC 3.4309 was used, purchased from China Common Microbial Species Collection and Management Center. Use conventional mold potato agar medium to cultivate spores on a slope to prepare a spore suspension (10 6 Pieces / mL), put into a 250mL Erlenmeyer flask containing 80mL enzyme production medium. Enzyme production fermentation medium formula (per L): inulin 50g / L, peptone 40g / L, sucrose ester 6g / L, pH 6.0. Under the condition of 30°C and 200 revolutions / minute, shake flask fermentation for 2-5 days, respectively take the fermentation broth of different culture days to determine the enzyme activity. And hydrolyze the Jerusalem artichoke syrup containing 8.5% total sugar at 50°C (adding enzyme amount 40U / g total sugar) to determine the reducing sugar after 4 hours, 8 hours, and 12 hours and calculate the hydrolysis rate. The results are shown in Table 1.

[006...

Example Embodiment

[0072] Example 2

[0073] Succinic Acid Fermentation for Preparation of Hydrolyzed Sugar from Jerusalem Artichoke by Different Hydrolysis Methods

[0074] Seed medium (per L): 5g glucose, 5g yeast extract, K 2 HPO 4 ·3H 2 O 1.0g, NaH 2 PO 4 ·2H 2 O 1.0g, pH 7.0. Anaerobic culture at 37°C for 16 hours.

[0075] Fermentation medium (per L): Jerusalem artichoke hydrolyzed syrup (total reducing sugar) 60g, yeast extract 15g, corn syrup 20g, MgCl 2 0.2g, Na 2 HPO 4 ·12H 2 O 1.5g, NaH 2 PO 4 ·2H 2 O1.5g, pH 6.5. Anaerobic culture at 37°C for 48 hours.

[0076] The method used in the preparation of enzymatic hydrolysis of Jerusalem artichoke syrup is briefly described as follows:

[0077] (1) Enzymatic hydrolysis: Wash the fresh tubers of Jerusalem artichoke, remove the mud, and roughly cut them to a size of 20-30 mm. Use a tissue masher to make a pulp of Jerusalem artichoke with a total sugar content of 5%-10%. Example 1 Prepared inulinase (44.1U / ml, 40U / g total sugar for the total in...

Example Embodiment

[0083] Example 3

[0084] Succinic acid fermentation of Jerusalem artichoke syrup with different initial reducing sugar concentrations

[0085] According to the method of Example 2, the inulinase-hydrolyzed fresh tuber hydrolyzed syrup of Jerusalem artichoke prepared in Example 1 was fermented in batches in an anaerobic flask. When the initial reducing sugar concentrations were 30, 45, 55, 65, and 80 g / L, respectively, The results are shown in Table 3:

[0086] Table 3 Succinic acid fermentation results of Jerusalem artichoke syrup with different initial reducing sugar concentrations (anaerobic flask batch fermentation)

[0087] Initial reducing sugar concentration

[0088] It can be seen from Table 3 that when the initial reducing sugar concentration is 65-80g / L, the output of succinic acid is the highest, reaching 46.54-46.61g / L. When the initial reducing sugar concentration is 65g / L, the succinic acid consumption The sugar yield was 79.1%, and the sugar utilization ...

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Abstract

The invention provides a method for producing butane diacid by fermenting cheap Jerusalem artichoke raw material. The invention also provides the application of the Jerusalem artichoke raw material in preparing butane diacid. Meanwhile, the invention provides the application of Actinobacillus succinogenes in the method for preparing the butane diacid by using the Jerusalem artichoke as raw material. The invention has the advantages of using renewable material, being environment-friendly, relieving the lack of the petrification resource in synthesizing the butane diacid by chemical process, and obvious embodiment of no competition with human for grains and no competition with grains for land; therefore, not only the cost of the culture medium of fermentation is reduced, but also the shortage of the grain as fermentation raw material is relieved; moreover, the development and use of large areas of marginal lands such as wasteland, desert and mudflat and the like are solved, thereby being favorable to environment and agriculture and rural construction, and increasing the income of farmers.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. Specifically, the present invention relates to a method for producing succinic acid by fermenting cheap Jerusalem artichoke raw materials. The invention also relates to the application of Jerusalem artichoke raw material in the preparation of succinic acid. The invention also relates to the application of Actinobacillus succinogenes in the method for preparing succinic acid from Jerusalem artichoke. Background technique [0002] Succinic acid, also known as succinic acid, has the molecular formula C 4 h 6 o 4 , with a molecular weight of 118.09, is a common natural organic acid that widely exists in the human body, animals, plants and microorganisms. [0003] Succinic acid is an important four-carbon compound in industry. It is widely used in food, medicine, pesticides, dyes, spices, paints, plastics and material industries as organic synthesis raw materials, intermediate products or ...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12N1/20C12R1/01
Inventor 过鑫富孙志浩
Owner HANGZHOU XINFU TECH CO LTD
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