Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Succinic acid-producing strain and its screening method and uses

A technology for producing succinic acid and strains, which is applied in the field of bioengineering and can solve problems such as difficulty in cultivation

Inactive Publication Date: 2009-09-09
NANJING TECH UNIV
View PDF2 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Typical gastrointestinal bacteria: E.coli, Pectinatus sp., Bacteroides sp.; rumen bacteria such as: Mannheimia succiniciproducens MBEL55E, Prevotella ruminicola (ATCC 19188), Succinimonas amylolytica (ATCC 19716), Ruminobacter amylophilus (DSM 1361), can accumulate D Diacid strains, but some of these strains are strictly anaerobic bacteria, which are relatively strict to equipment and culture conditions, and are not easy to cultivate. Some acid production levels are too low, and the concentration of substrates and products is too high. There are some deficiencies in industrialized production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Succinic acid-producing strain and its screening method and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Weigh 1.0 g of the strain-containing mixture obtained from the rumen of the cow, and add it to 50 ml of sterile water to prepare a rumen bacteria suspension. Take 1ml of rumen bacteria suspension into a 100ml serum bottle, the amount of enrichment medium in the serum bottle is 50ml, and the composition of the enrichment medium is: NaCl 1.0g / L, K 2 HPO 4 4.0g / L, MgCl 2 ·6H 2 O 0.05g / L, CaCl 2 ·H 2 O0.05g / L, (NH 4 ) 2 SO 4 0.2g / L, yeast extract 10g / L, peptone 5.0g / L, glucose 50.0g / L, monensin 0.2mg / L, disodium fumarate 20.0g / L, disodium succinate 60.0 g / L, magnesium carbonate 50g / L; after enrichment culture at 37°C for 18h, the culture sample was diluted 10 -6 , take a 0.1ml droplet spread on the flat plate containing the separation medium, and cultivate it in an anaerobic incubator. 12 20mg / L, lipoic acid 20mg / L, niacin 20mg / L, pantothenic acid 20mg / L, thiamine 20mg / L, vitamin B6 20mg / L, agar 20g / L; mixed gas N in the anaerobic incubator 2 :CO 2 :H 2 =8:1:...

Embodiment 2

[0050] Seed medium: yeast extract 5g / L, peptone 5g / L, NaCl 1g / L, NaHCO 3 10g / L, Na 2 HPO 4 9~15g / L,KH 2 PO 4 4~12g / L, add agar 20g / L when cultivating on a slant surface, and inject N 2 :CO 2 =4:1 mixed gas for 2 minutes, sterilized at 121°C for 20 minutes, and cooled.

[0051] Fermentation medium: glucose 100g / L, MnCl 2 0.05g / L, FeCl 2 0.05g / L, CaCl 2 0.05g / L, K 2 HPO 4 2g / L, MgCO 3 100g / L, vitamins added folic acid 20mg / L, biotin 20mg / L, vitamin B 12 20mg / L, lipoic acid 20mg / L, niacin 20mg / L, pantothenic acid 20mg / L, thiamine 20mg / L, vitamin B 6 20mg / L, yeast extract and peptone respectively use the following dosages:

[0052] (1) 10g / L yeast extract, 5g / L peptone;

[0053] (2) 15g / L yeast extract, 5g / L peptone;

[0054] (3) 10g / L yeast extract, 15g / L peptone;

[0055] The volume of 100ml serum bottle is 50ml, and N 2 :CO 2 =4:1 mixed gas for 2 minutes, sterilized at 121°C for 20 minutes, and cooled.

[0056] After streaking the strain with the pre...

Embodiment 3

[0061] Seed medium: yeast extract 5g / L, peptone 5g / L, NaCl 1g / L, NaHCO 3 10g / L, Na 2 HPO 4 9~15g / L,KH 2 PO 4 4~12g / L, (add agar 20g / L when cultivating on a slant), pass through N 2 :CO 2 = 4:1 mixed gas for 2 minutes, sterilized at 121°C for 20 minutes, and cooled.

[0062] Fermentation medium: glucose 100g / L, yeast extract 10-50g / L, peptone 10-50g / L, MnCl 2 0.05g / L, FeCl 2 0.05g / L, CaCl 2 0.05g / L, K 2 HPO 4 2.0g / L, MgCO 3 100g / L, vitamins added folic acid 5mg / L, biotin 5mg / L, vitamin B 125mg / L, lipoic acid 5mg / L, niacin 5mg / L, pantothenic acid 5mg / L, thiamine 5mg / L, vitamin B 6 5mg / L, pH7.0.

[0063] When cultivating in a 5L fermenter, the liquid volume of the medium is 3L, with 100g / L MgCO 3 As a pH buffer, the fermenter gas mixture (N 2 :CO 2 =4:1) The ventilation rate is 0.6L / min to maintain the anaerobic environment of the fermentation system. After streaking the strain with the preservation number CGMCC No.1716 on the seed slant medium, culture ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a strain producing succinic acid, a screening method and application thereof. The strain is classified as Actinobacillus succinogenes NJ113, and has been preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.1716. In the invention, the bacterial strains in the animal rumen are enriched and cultivated in the medium containing disodium fumarate and then classified to obtain actinomycetes with high production of succinic acid. Fermentation by using the bacterial strain can produce succinic acid with higher concentration, which is suitable for industrial production of succinic acid.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a strain producing succinic acid. The invention also relates to a screening method for the strain and a method for fermenting and producing succinic acid using the strain. Background technique [0002] As an important chemical raw material, succinic acid (also known as succinic acid) is widely used in industries such as medicine, food and surfactant. However, the traditional chemical synthesis of succinic acid has limited the wide application of succinic acid as a basic chemical raw material due to reasons such as cost and environmental pollution. Because of this, the research and development of succinic acid production by microbial fermentation is being carried out in depth in the United States, Japan and other countries. [0003] The production of succinic acid by fermentation has many advantages. First of all, the new microbial fermentation production process and the ne...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/46
Inventor 姜岷韦萍陈可泉苏溧蔡婷王倩楠欧阳平凯
Owner NANJING TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com