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Method for producing succinic acid by multistage continuous fermentation

A succinic acid and fermenter technology, applied in the field of succinic acid preparation, can solve the problems of high cost, serious pollution, unsustainable development and the like, and achieve the effects of easy operation, simple method and high yield

Inactive Publication Date: 2014-11-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chemical method mainly uses non-renewable petroleum resources as raw materials, which has high cost, serious pollution and unsustainable development, which seriously limits the development of succinic acid

Method used

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  • Method for producing succinic acid by multistage continuous fermentation
  • Method for producing succinic acid by multistage continuous fermentation
  • Method for producing succinic acid by multistage continuous fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for the production of succinic acid by a multistage continuous fermentation device, comprising the following steps:

[0029] The first step is to prepare seed medium, initial fermentation medium, carbon source feed tank culture fluid and medium feed tank culture fluid, the formula is as follows:

[0030] Seed medium (g L -1 ): glucose 10 (divided), yeast extract 5, corn steep liquor 5, NaHCO 3 10, NaH 2 PO 4 2H 2 O 9.6, K 2 HPO 4 ·3H 2 O 15.5, pH 7.0.

[0031] Initial fermentation medium (g L -1 ): glucose 40 (divided), yeast extract 10, corn steep liquor 10, sodium acetate 1.36, KH 2 PO 4 3. MgCl 2 ·6H 2 O 0.2, CaCl 2 0.2, NaCl 1, Na 2 HPO 4 12H 2 O 0.31, NaH 2 PO 4 2H 2 O 1.6, pH 7.0.

[0032] Carbon source feeding tank culture fluid (g L -1 ): Glucose 750.

[0033] Culture medium feed tank culture solution (g L -1 ): yeast extract 30, corn steep liquor 30, sodium acetate 4.08, KH 2 PO 4 9. MgCl 2 ·6H 2 O 0.6, CaCl 2 0.6,...

Embodiment 2

[0038] The first step to the third step are the same as in Example 1, wherein the culture fluid of the carbon source feeding tank is 200 g / L sucrose solution.

[0039] In the fourth step, after five sterilized fermenters are connected in series, 600ml of initial fermentation medium is inserted into the primary fermenter, and CO with a volume ratio of 1:1 is introduced. 2 and H 2 Mixed gas, using the gas storage tank to control the ventilation rate to 0.01L / (min L), according to the inoculation amount of 10% (v / v) into the fermentation strain, the initial fermentation medium flows from the primary fermentation tank into the secondary, From the third level to the fifth level, the carbon source feeding tank and the medium feeding tank are used to control the inflow rate of the fermentation broth to 120-150 ml / h, the rate of sucrose supplementation is 30 ml / h, and the outflow rate of the fermentation broth is 150-160 ml / h h, so that the initial sugar concentration in the primary ...

Embodiment 3

[0041] The first step to the third step are the same as in Embodiment 2.

[0042] The fourth step is to connect the five sterilized fermentation tanks in series, insert 600ml of initial fermentation medium into the primary fermentation tank, and use the gas storage tank to control pure N2, and the ventilation rate is 0.04 L / (min·L) According to the inoculation amount of 10% (v / v), the fermentation strains are inserted, and the initial fermentation medium flows from the first-level fermentation tank into the second-level, third-level and fifth-level fermentation tanks, and is controlled by the carbon source feeding tank and the medium feeding tank The inflow rate of the fermentation broth is 90-110 ml / h, the rate of sucrose supplementation is 20 ml / h, and the outflow rate of the fermentation broth is 110-140ml / h, so that the initial sugar concentration in the primary fermentation tank is 80g / L. The sugar concentration in the fermentation tanks at all levels relative to the init...

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Abstract

The invention discloses a method for producing succinic acid by multistage continuous fermentation. The method comprises the following steps: firstly, preparing a seed culture medium, a primary fermentation culture medium, a carbon source replenishing tank culture liquid and a medium replenishing tank culture liquid; secondly, sterilizing a serial fermentation tank set, a carbon source replenishing tank, a culture medium replenishing tank, the seed culture medium, the primary fermentation culture medium, the carbon source replenishing tank culture liquid, the medium replenishing tank culture liquid and a serum bottle; thirdly, inoculating the sterilized seed culture medium into the sterilized serum bottle, introducing CO2, inoculating actinobacillus succinogenes NJ113, and performing shake cultivation to serve as a fermentation strain; fourthly, performing multistage continuous fermentation. As the production method is simple and the yield and purity of succinic acid are high, the method is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of succinic acid preparation, in particular to a method for producing succinic acid by multistage continuous fermentation. Background technique [0002] Succinic acid, also known as succinate acid, is a common natural organic acid and one of the intermediate metabolites of the tricarboxylic acid cycle, which is widely found in animals, plants and microorganisms. As an important C4 platform compound, succinic acid is an important intermediate product and 1,4-butanediol (BDO), γ-butyrolactone, tetrahydrofuran, N-methylpyrrolidone (NMP), adipic acid , malic acid and the new biodegradable plastic product polybutylene succinate (PBS) and other specialty chemical precursors, the total market for these chemicals is 15 billion US dollars. The latest research results also show that succinic acid has very broad prospects in the preparation and application of biodegradable plastics. The polymerization reaction of succinic acid...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12R1/01
CPCC12P7/46
Inventor 陈可泉王震何珣肖文欧阳平凯
Owner NANJING UNIV OF TECH
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