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Bacterial strain capable of enhancing glycerin metabolism and application thereof

A glycerol metabolism and strain technology, applied in bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as accumulation and inhibition of microbial growth, and achieve recovery of growth ability, change of growth and metabolic performance, high efficiency The effect of using

Active Publication Date: 2016-08-10
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, if the reduction of the synthetic product is low (such as succinic acid), a large amount of NADH will be accumulated in the bacteria, and the excess reducing power will inhibit the growth of microorganisms

Method used

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  • Bacterial strain capable of enhancing glycerin metabolism and application thereof
  • Bacterial strain capable of enhancing glycerin metabolism and application thereof
  • Bacterial strain capable of enhancing glycerin metabolism and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example illustrates the construction method of the Actinobacillus succinogenes JF1315 strain of the present invention.

[0027] The starting strain Actinobacillus succinogenes NJ113 strain used in the screening of JF1315 strain in the present invention has been disclosed in the patent previously applied by the same inventor, the patent number is ZL200610085415.9, and the preservation number is CGMCC No.1716.

[0028] Actinobacillus succinogenes JF1315 strain was screened from Actinobacillus succinogenes NJ113 by ARTP mutagenesis.

[0029] The specific mutagenesis screening steps are as follows:

[0030] ARTP mutagenesis: the starting strain Actinobacillus succinogenes NJ113 was inoculated in an anaerobic serum bottle containing seed medium and cultured for 6-12 hours to obtain a strain in logarithmic growth phase. Properly dilute the seed culture solution to OD 660= 0.5-1.5 and then spread on the cooled slide for mutagenesis. The mutagenesis conditions were sele...

Embodiment 2

[0037] This embodiment illustrates the physiological and biochemical characteristics of Actinobacillus succinogenes JF1315 obtained in the above-mentioned embodiment 1, specifically as follows:

[0038] There was no significant difference in colony morphology and growth performance between the mutagenized strain and the original bacteria Actinobacillus succinogenes NJ113: the strain was Gram-negative, and the plate colony was round with smooth edges, and could metabolize glucose and xylose to synthesize organic acids under anaerobic conditions. The main acid composition is succinic acid, acetic acid, lactic acid and formic acid. The strain is glutamate-deficient and requires the addition of glutamate for growth on synthetic media.

[0039] The genetic stability test of Actinobacillus succinogenes JF1315 in Example 1, the strain subculture and fermentation experiments are as follows.

[0040] The mutagenized strain Actinobacillus succinogenes JF1315 was continuously subculture...

Embodiment 3

[0044] This example illustrates the superiority of the mutagenized strain Actinobacillus succinogenes JF1315 in the present invention compared to the starting strain.

[0045] In the present invention, the Actinobacillus succinicum NJ113 and JF1315 strains are cultured on a solid plate medium and then inoculated into a seed medium for cultivation to obtain a seed liquid; then the seed liquid is inoculated into a low-pH fermentation medium for anaerobic fermentation. The method may include the steps of:

[0046] (1) Actinobacillus succinogenes NJ113 and JF1315 strains were activated on solid plate medium and then transferred to anaerobic serum bottle, cultured under anaerobic conditions at 37°C for 12-14 hours, then transferred to seed medium, at 37 Cultivate at 200 rpm for 6-8 hours to obtain seed liquid;

[0047] (2) Inoculate the above-mentioned seed liquid into a serum bottle containing a low pH fermentation medium (pH5.8) according to the inoculation amount of 6-10 % (v / v...

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Abstract

The invention discloses a bacterial strain capable of enhancing glycerin metabolism. The bacterial strain is classified and names as Actinobacillussuccinogenes JF1315, already collected at the China Center for Type Culture Collection on 31th, Mar. 2016 under CCTCC NO: M 2016160. The invention further relates to application of Actinobacillussuccinogenes JF1315 in producing organic acid through fermentation. Compared with control bacterial strains, under an anaerobic condition, the bacterial strain can grow and metabolizes at low pH, and reductive organic acid can be synthesized efficiently by utilizing glycerin fermentation. When pH is 4.8-6.8, the bacterial strain can normally grow and metabolize glucose to synthesize the organic acid like succinic acid; in addition, under the condition, the bacterial strain can efficiently utilize glycerin for anaerobic fermentation to synthesize and accumulate the organic acid.

Description

technical field [0001] The invention relates to a bacterial strain that strengthens glycerol metabolism and its application, belonging to the technical field of industrial microbes and their fermentation. Background technique [0002] In the traditional engineering practice in the field of microbial fermentation, carbohydrates are generally used as carbon sources for fermentation, such as corn starch hydrolyzate; for some high value-added products, glucose is even directly used as raw material for product synthesis . Although a product with a higher concentration can be obtained when using raw materials such as glucose for fermentation, this greatly increases the economic cost of the entire production process and causes a waste of resources. In order to solve this problem, cheap biomass materials (such as cellulose hydrolyzate or glycerol) can be tried to replace glucose for fermentation, but the existence of toxic substances and low sugar concentration in cellulose hydroly...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/46C12P7/40C12R1/01
CPCC12P7/40C12P7/46C12R2001/01C12N1/205
Inventor 姜岷冀亚亮马江锋陈美丽
Owner NANJING UNIV OF TECH
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