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Method for producing succinic acid by using ferment of molasses raw material

A technology of succinic acid and molasses, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, to achieve the effect of alleviating the shortage of petrochemical resources and reducing the cost of culture medium

Inactive Publication Date: 2007-12-26
HANGZHOU XINFU TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no industrial example of using molasses raw material to ferment succinic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Seed medium (per L): glucose 5g, yeast extract 5g, K 2 HPO 4 ·3H 2 O 1.0g, NaH 2 PO 4 2H 2 O1.0g, mixed vitamins 1ml, pH7.0. 37 ℃ anaerobic culture for 24h.

[0033] Fermentation medium (per L): processed sugarcane molasses (total reducing sugar) 65g, yeast extract 15g, corn steep liquor 20g, MgCl 2 0.2g, Na 2 HPO 4 12H 2 O 1.5g, NaH 2 PO 4 2H 2 O 1.5g, pH 6.5. Anaerobic culture at 37°C for 48h.

[0034] The molasses is processed as follows:

[0035] First, dilute raw molasses (about 510g / L reducing sugar) with tap water to a sugar concentration of 300g / L (hereinafter referred to as diluted molasses), and then use the following methods to process it.

[0036] (1) Sulfuric acid treatment: the diluted molasses was adjusted to pH 3.5 with 1 mol / L sulfuric acid solution, left to stand for 6 hours, centrifuged, and the supernatant was adjusted to pH 6.5 with 5 mol / L NaOH.

[0037] (2) Potassium ferrocyanide treatment: Add 0.1 g of potassium ferrocyanide to 100...

Embodiment 2

[0045] According to the method of Example 1, in the anaerobic bottle fermentation experiment, use sulfuric acid to treat sugarcane molasses, the initial reducing sugar concentration is 65g / L, add different inorganic and organic nitrogen sources respectively in the fermentation medium (its total nitrogen content is basically similar), and their contents are (per L): NH 4 Cl 5.3g, KNO 3 9.6g, 12g of peptone, 25g of corn steep liquor (solid content ≥ 55%), 15g of yeast extract and 12g of dry yeast powder. The effects of various nitrogen sources on the production of succinic acid from fermented sugarcane molasses are shown in Table 2.

[0046] Table 2 Effects of adding different nitrogen sources on the production of succinic acid from fermented sugarcane molasses (fermentation in anaerobic bottles)

[0047]

[0048] Compared with the above several nitrogen sources, yeast extract is the best. When the concentration of yeast extract is 15g / L, the output of succinic acid...

Embodiment 3

[0050] According to the method of Example 1, in the anaerobic bottle fermentation experiment, use sulfuric acid to process sugarcane molasses, when the initial reducing sugar concentration is respectively 35,50,65,80,100g / L, the results are as shown in table 3:

[0051] Table 3 Effects of different initial reducing sugar concentrations on the production of succinic acid from sugarcane molasses (anaerobic bottle fermentation)

[0052] initial reducing sugar concentration

[0053] It can be seen from the above table that when the initial reducing sugar concentration is 65g / L, the yield of succinic acid is the highest, reaching 50.6g / L, the yield of succinic acid to consumed sugar is 83.70%, the utilization rate of sugar is 93.0%, and the production intensity is 1.05g / L·h.

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PUM

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Abstract

This invention discloses a method for producing succinic acid from waste syrup by fermentation. The method comprises: screening rumen to obtain Actinobacillus succinogenes (CGMCC 1593), and performing fed batch anaerobic fermentation on waste syrup (reducing sugar concentration = 50-100 g / L) in the presence of CO2 or N2 at a pH value of 5.5-7.5 for 30-60 h. The concentration of succinic acid can reach 30-60 g / L. The conversion rate is 70-84%, the productivity is 0.9-1.2 g / L.h, and the sugar utilization rate is 90-95%. The method utilizes renewable resource (waste syrup) instead of pure glucose as the carbon source to produce succinic acid. The method has a low cost, and is environmentally friendly.

Description

technical field [0001] The invention relates to a method for producing succinic acid by fermenting molasses raw materials, which belongs to the technical field of biomanufacturing. Background technique [0002] Succinic acid (succsinic acid), also known as succinic acid, the molecular formula is C 4 h 6 o 4 , with a molecular weight of 118.09, is a common natural organic acid that widely exists in the human body, animals, plants and microorganisms. [0003] Succinic acid is an important four-carbon compound in industry. It is widely used in food, medicine, pesticides, dyes, spices, paints, plastics and material industries as organic synthesis raw materials, intermediate products or professional chemicals. Its largest market is used in surfactants, detergents, green solvents, ion chelating agents, biodegradable plastics and other fields. It is used in the food industry as an acidulant, pH modifier, flavoring substance and antibacterial agent, and as a raw material or inte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12R1/01
Inventor 孙志浩郑璞刘宇鹏
Owner HANGZHOU XINFU TECH CO LTD
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