30 results about "Phosphoketolase" patented technology

The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and / or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

The invention relates to a recombinant yeast, a construction method thereof and application thereof for producing tyrosol and a derivative. The recombinant yeast is acquired by introducing an exogenous fructose-6-phosphate phosphoketolase into an engineered yeast cell, and the engineered yeast cell is a yeast cell having a metabolic pathway for the synthesis of tyrosol via erythrose-4-phosphate and phosphoenolpyruvate. It is disclosed for the first time that in the process of expressing the fructose-6-phosphate phosphoketolase in the yeast, fructose-6-phosphate is catalyzed into erythrose-4- phosphate and acetyl phosphate while synthesizing 1,6-fructose diphosphate, xylulose-5-phosphate is catalyzed into glyceraldehydes-3-phosphate and acetyl phosphate, which alters the distribution of carbon metabolism in yeast, and enhances the synthesis of erythrose-4-phosphate, an important intermediate of tyrosol biosynthesis. The metabolic pathway of synthetic tyrosol is optimized, and the yieldof derivatives such as tyrosol and hydroxytyrosol is improved.

Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.

Substance productivity is improved by introducing a metabolic pathway for synthesis of acetyl-CoA or acetic acid from glucose-6-phosphate into yeast. Acetic acid productivity, acetyl-CoA productivity, and productivity of a substance made from acetyl-CoA-derived are improved by attenuating genes involved in the glycolytic system of yeast and introducing a phosphoketolase gene into the yeast.

Substance productivity is improved by introducing a metabolic pathway for synthesis of acetyl-CoA or acetic acid from glucose-6-phosphate into yeast. Acetic acid productivity, acetyl-CoA productivity, and productivity of a substance made from acetyl-CoA-derived are improved by attenuating genes involved in the glycolytic system of yeast and introducing a phosphoketolase gene into the yeast.

The invention provides phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids. This present invention relates to cultured recombinant cells comprising a heterologous phosphoketolase (PKL) polypeptide that are capable of increased production of acetyl coenzyme A-derived metabolites, as well as methods for producing and using the same. In some embodiments, the recombinant cells further comprise one or more mevalonate (MVA) pathway polypeptides for the production of isoprenoid precursors, isoprene and isoprenoids.

The invention discloses a phosphoketolase with improved activity and its use in the production of metabolites. The protein provided by the present invention is a mutant protein, which is a protein obtained by subjecting phosphoketolase to any one or more of the following mutations: the second position is mutated from T to A; the sixth position is mutated from I to T; the 14th position The position is mutated from N to D; (the 20th position is mutated from E to D; the 120th position is mutated from T to A; the 231st position is mutated from E to K; the 260th position is mutated from H to Y; the 342nd position is mutated from E It is K; the 397th position is mutated from K to R; the 676th position is mutated from D to G; the 785th position is mutated from F to L; the 801st position is mutated from W to R. The present invention also protects the mutant protein when preparing metabolites Application in. Compared with the existing phosphoketolase, the phosphoketolase activity of the mutant protein provided by the invention is significantly increased, thereby significantly increasing the yield of target metabolites.

The invention discloses a method for increasing the cell phloroglucinol synthesis yield and application, and belongs to the technical field of genetic engineering. According to the method provided by the invention, a recombined cell co-expresses a phosphoketolase fxpk gene, a fructose 1,6-biphosphatase fbp gene and a poly-ketene anhydride synthetase phld gene and also expresses any one or two of a multiple-resistance-factor MarA gene and an acetyl-coenzyme a carboxylase ACCase gene. According to the method provided by the invention, a new phloroglucinol synthesis way is built in the recombined cell, so that the yield of phloroglucinol is greatly increased; as for a contrast group, the phloroglucinol yield is increased by 68.9 percent; meanwhile, by the method, emission of carbon dioxide in a phloroglucinol synthesis process is also reduced; the method has high environmental benefits.