Method for increasing cell phloroglucinol synthesis yield and application

A phloroglucinol and cell technology, applied in the field of genetic engineering, can solve the problems of unknown metabolism, phloroglucinol production and yield, etc., and achieve the effect of improving capacity and reducing production and emission
CN104357495AActive Publication Date: 2015-02-18QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
4 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Publication Date
2015-02-18

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention discloses a method for increasing the cell phloroglucinol synthesis yield and application, and belongs to the technical field of genetic engineering. According to the method provided by the invention, a recombined cell co-expresses a phosphoketolase fxpk gene, a fructose 1,6-biphosphatase fbp gene and a poly-ketene anhydride synthetase phld gene and also expresses any one or two of a multiple-resistance-factor MarA gene and an acetyl-coenzyme a carboxylase ACCase gene. According to the method provided by the invention, a new phloroglucinol synthesis way is built in the recombined cell, so that the yield of phloroglucinol is greatly increased; as for a contrast group, the phloroglucinol yield is increased by 68.9 percent; meanwhile, by the method, emission of carbon dioxide in a phloroglucinol synthesis process is also reduced; the method has high environmental benefits.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method and application for increasing the synthesis yield of cell phloroglucinol, belonging to the technical field of genetic engineering. Background technique

[0002] Phloroglucinol, also known as 1,3,5-trihydroxybenzene and pyroglucinol, is an important fine chemical product and can be used as an intermediate in drug synthesis, fuel coupling agent, tire tackifier and azo composite ink It is widely used in the dyeing process of textiles and leather, the production of plastic capsules, and the replacement of silver iodide for artificial rainfall. In addition, phloroglucinol itself is an excellent pharmaceutical product, an anti-curing agent with superior performance, and has been widely used in antibacterial and antiseptic.

[0003] As early as the 1950s, the chemical synthesis process of phloroglucinol had been established and applied to industrial production, including 2,4,6-trinitrotoluene (TNT) pathway, 1,3,5-triiso Pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More