Inorganic phosphorus (phosphate radical) diagnostic and measuring kit and method for measuring inorganic phosphorus (phosphate radical) concentration

Abstract

The invention relates to an inorganic phosphorus (phosphate radical) diagnostic and measuring kit utilizing technologies of an enzymatic recycling method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring inorganic phosphorus (phosphate radical) concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science, food and environment. The kit mainly comprises the following components: buffer solution, reduced coenzyme, oxaloacetic acid, acetyl phosphate, hydrogen peroxide, phosphoenolpyruvate carboxylase (PEPC), pyruvic oxidase, malate dehydrogenase and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the inorganic phosphorus (phosphate radical) concentration.

Description

technical field

[0001] The invention relates to an inorganic phosphorus (phosphate) diagnosis / determination kit, and also relates to a method for measuring the concentration of inorganic phosphorus (phosphate), belonging to the technical field of medical / food / environmental inspection and determination. Background technique

[0002] 87% of the phosphorus in the human body exists in the bones. The phosphorus in the blood and the phosphorus in the bones maintain a dynamic balance. There is a certain relationship between the concentration of calcium and phosphorus in the blood. The increase of blood calcium in normal people will decrease the blood phosphorus, and vice versa. Of course. The product of [calcium] and [phosphorus] concentrations in plasma is kept within a certain range; approximately the product of plasma calcium and phosphorus concentrations per 100 ml is 35-40. When the product of the two is greater than 40, calcium and phosphorus are deposited in bone tissue in ...