Inorganic phosphorus (phosphate radical) diagnostic and measuring kit and method for measuring inorganic phosphorus (phosphate radical) concentration

A technology of phosphate and inorganic phosphorus, which is applied in the field of inorganic phosphorus diagnosis/determination kits and the determination of inorganic phosphorus concentration, and can solve the problem of high results

Inactive Publication Date: 2009-12-23
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, jaundice, hemolysis, and fat-turbid serum have light absorption at 340nm, and a blank specimen must be prepared, otherwise the result will be high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The inorganic phosphorus (phosphate) diagnostic / measurement reagent of this embodiment is a single reagent, including:

[0047] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0048] Stabilizer 500mmol / L

[0049] Reduced coenzyme 0.25mmol / L

[0050] Phosphoenolpyruvate carboxylase 6000U / L

[0051] Pyruvate oxidase 8000U / L

[0052] Malate Dehydrogenase 10000U / L

[0053] Oxaloacetate 16mmol / L

[0054]Acetyl phosphate 9mmol / L

[0055] Hydrogen peroxide 6mmol / L

[0056] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0057] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, volume ratio of measured inorganic phosphorus (phosphate) sample to reagent It is 1 / 25, the reaction direction is n...

Embodiment 2

[0060] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is double reagent, comprises:

[0061] Reagent 1

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 50mmol / L

[0064] Reduced coenzyme 0.25mmol / L

[0065] Oxaloacetate 16mmol / L

[0066] Acetyl phosphate 9mmol / L

[0067] Hydrogen peroxide 6mmol / L

[0068] Reagent 2

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 500mmol / L

[0071] Phosphoenolpyruvate Carboxylase 6000U / L

[0072] Pyruvate oxidase 8000U / L

[0073] Malate dehydrogenase 10000U / L

[0074] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0075] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm,...

Embodiment 3

[0078] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is three reagents, comprises:

[0079] Reagent 1

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 50mmol / L

[0082] Reduced coenzyme 0.25mmol / L

[0083] Oxaloacetate 16mmol / L

[0084] Acetyl phosphate 9mmol / L

[0085] Hydrogen peroxide 6mmol / L

[0086] Reagent 2

[0087] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0088] Stabilizer 500mmol / L

[0089] Pyruvate oxidase 8000U / L

[0090] Malate dehydrogenase 10000U / L

[0091] Reagent 3

[0092] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0093] Stabilizer 500mmol / L

[0094] Phosphoenolpyruvate Carboxylase 6000U / L

[0095] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0096] When measuring the concentration of inorganic ph...

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PUM

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Abstract

The invention relates to an inorganic phosphorus (phosphate radical) diagnostic and measuring kit utilizing technologies of an enzymatic recycling method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring inorganic phosphorus (phosphate radical) concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science, food and environment. The kit mainly comprises the following components: buffer solution, reduced coenzyme, oxaloacetic acid, acetyl phosphate, hydrogen peroxide, phosphoenolpyruvate carboxylase (PEPC), pyruvic oxidase, malate dehydrogenase and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV/visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the inorganic phosphorus (phosphate radical) concentration.

Description

technical field [0001] The invention relates to an inorganic phosphorus (phosphate) diagnosis / determination kit, and also relates to a method for measuring the concentration of inorganic phosphorus (phosphate), belonging to the technical field of medical / food / environmental inspection and determination. Background technique [0002] 87% of the phosphorus in the human body exists in the bones. The phosphorus in the blood and the phosphorus in the bones maintain a dynamic balance. There is a certain relationship between the concentration of calcium and phosphorus in the blood. The increase of blood calcium in normal people will decrease the blood phosphorus, and vice versa. Of course. The product of [calcium] and [phosphorus] concentrations in plasma is kept within a certain range; approximately the product of plasma calcium and phosphorus concentrations per 100 ml is 35-40. When the product of the two is greater than 40, calcium and phosphorus are deposited in bone tissue in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/25G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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