A new pathway for the synthesis of acetyl-CoA and its derivatives from glycolaldehyde

A technology of acetyl coenzyme and glycolaldehyde, applied in the field of biomedicine, can solve the problems of low reaction rate, low efficiency, and difficulty in accumulating high concentration of glycolaldehyde, etc., and achieve the effects of high catalytic activity, high reaction efficiency and strong affinity

Active Publication Date: 2020-07-28
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The reaction rate of the preparation method of above-mentioned acetyl phosphate is lower, and the conversion number K of phosphoketolase is for fructose cat Only 0.1 / s, the efficiency is very low, and the long reaction time (18h) will test the stability of the enzyme and product
In addition, the third step, the cracking of D-erythrose into glycolaldehyde is a reversible process, and its reaction rate is affected by the concentration of glycolaldehyde after cracking, making it difficult to accumulate high concentrations of glycolaldehyde in the system, which will lead to the formation of acetyl phosphate The speed is difficult to guarantee
The acetyl phosphate generated by the second enzymatic reaction is likely to affect the yield of the last enzymatic reaction due to product inhibition. If the second enzymatic reaction generates acetyl phosphate and removes it immediately, it will complicate the entire preparation process.

Method used

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  • A new pathway for the synthesis of acetyl-CoA and its derivatives from glycolaldehyde
  • A new pathway for the synthesis of acetyl-CoA and its derivatives from glycolaldehyde
  • A new pathway for the synthesis of acetyl-CoA and its derivatives from glycolaldehyde

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Experimental program
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Effect test

Embodiment 1

[0078] At an initial concentration of glycolaldehyde of 15 mM, the mutant enzyme Tal B of Transaldolase Tal B derived from Escherichia coli F178Y (On the basis of the original enzyme, the 178th amino acid residue is mutated from F to Y), add 5mM G3P, and carry out the preparation reaction of Ara5. After 1 hour of reaction, the reaction solution is lyophilized and derivatized, and quantitatively detected by GC-MS The obtained Ara5P content was 2.5mM, and the average synthesis rate of Ara5P was 52.1μmol(Ara5P) / min / mg(enzyme protein).

Embodiment 2

[0080] Glycolaldehyde at an initial concentration of 15mM, ThDP concentration was 1mM, PO 4 3+ The concentration was 2mM, and Fpk (EC 4.1.2.22) derived from Bifidobacterium adolescentis, aldolase (Fsa, EC 4.1.2.13) derived from Escherichia coli, 2mM G3P and trace amounts of Rpi A and Rpe were added to prepare AcP. After testing, the conversion rate of glycolaldehyde was 16.3 μmol (glycolaldehyde) / min / mg (enzyme protein).

[0081] The above data is the average rate of the reaction system for 3 hours.

Embodiment 3

[0083] Glycolaldehyde at an initial concentration of 15mM, ThDP concentration was 1mM, PO 4 3+ The concentration is 2mM, add the mutant enzyme Tal B of Xpk (EC 4.1.2.9) derived from Pseudomonas stutzeri A1501 and Transaldolase (Tal B, EC 2.2.1.2) derived from Escherichia coli F178Y , 2mM G3P and trace amounts of Rpi A and Rpe to prepare AcP. After testing, the conversion rate of glycolaldehyde was 19.7 μmol (glycol aldehyde) / min / mg (enzyme protein).

[0084] The above data is the average rate of the reaction system for 3 hours.

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Abstract

Disclosed is a new approach for synthesizing an acetyl coenzyme A and a derivative product thereof (5-phosphoarabinose, acetyl phosphoric acid, acetyl phosphate, and an acetyl coenzyme A derivative compound) using glycolaldehyde. The approach comprises a reaction of glycolaldehyde and 3-phosphoglyceraldehyde under the catalysis of an enzyme to generate 5-phosphoarabinose, wherein the enzyme is selected from an aldolase, a transaldolase, an isoenzyme and a mutant enzyme thereof.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for synthesizing acetyl coenzyme A and derivatives thereof by using glycolaldehyde. Background technique [0002] Acetyl coenzyme A (AcCoA) is a key intermediate in the synthesis of essential biological compounds, including polyketides, fatty acids, isoprenoids, alkaloids, vitamins, and amino acids, among others. Metabolites derived from AcCoA are primary and secondary metabolites, which include compounds of industrial utility. AcCoA can be generated from acetyl phosphate (AcP) (for example, it can be catalyzed by phosphoacetyltransferase Pta (EC 2.3.1.8)), and then generate a series of biological products based on AcCoA platform, which are widely used in biocatalysis and other fields. The synthesis methods of AcP are: [0003] Patent application WO2015 / 144447A1 discloses a phosphoketolase (EC 4.1.2.9, fructose-6-phosphate phosphoketolase EC4.1.2.22) or sulfoacetal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02C12P9/00C12P19/32C12P7/04C12P7/28C12P7/54C12P7/62C12P13/06C12P13/10C12P13/12C12P13/14C12P13/24
CPCC12P7/04C12P7/28C12P7/54C12P7/62C12P7/625C12P9/00C12P13/06C12P13/10C12P13/12C12P13/14C12P13/24C12P19/02C12P19/32
Inventor 马红武杨雪袁倩倩江会锋
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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