Inorganic phosphorus (phosphate radical) diagnosis/determination kit and method for determining inorganic phosphorus (phosphate radical) concentration

Abstract

The invention relates to an inorganic phosphorus (phosphate radical) diagnosis / determination kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining inorganic phosphorus (phosphate radical) concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food, and environment. The kit comprises the following main components: buffer solution, coenzyme, adenoside bisphosphate, oxaloacetic acid, acetyl phosphate, hydrogen peroxide, coenzyme A, pyruvate carboxylase, pyruvate oxidase, pyruvate dehydrogenase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the inorganic phosphorus (phosphate radical) concentration.

Description

technical field

[0001] The invention relates to an inorganic phosphorus (phosphate) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring the concentration of the inorganic phosphorus (phosphate), which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique

[0002] 87% of the phosphorus in the human body exists in the bones. The phosphorus in the blood and the phosphorus in the bones maintain a dynamic balance. There is a certain relationship between the concentration of calcium and phosphorus in the blood. When the blood calcium increases in normal people, the blood phosphorus decreases, and vice versa. Of course. The product of [calcium] and [phosphorus] concentrations in plasma remains within a certain range; When the product of the two is greater than 40, calcium and phosphorus are deposited in the bone tissue in the form of bone salt, and when the product is greater...